公开(公告)号：US20160265010A1

公开(公告)日：2016-09-15

申请号：US15073641

申请日：2016-03-18

申请人： WILLIAM MARSH RICE UNIVERSITY

发明人： Ka-Yiu SUN ,  Mai LI ,  Xiujun ZHANG

IPC分类号： C12P7/64 ,  C12N9/42 ,  C12N15/70 ,  C12N15/52

CPC分类号： C12P7/6409 ,  C12N9/16 ,  C12N9/2442 ,  C12N15/52 ,  C12N15/70 ,  C12Y301/02014 ,  C12Y302/01014 ,  Y02P20/52

摘要： There is provided a recombinant bacterium comprising at least one overexpressed acyl-ACP thioesterase gene, and wherein at least one gene from the tricarboxylic acid cycle or glycolysis or both is inactivated. There is also provided a method for producing fatty acids, said method comprising culturing bacteria comprising at least one overexpressed acyl-ACP thioesterase gene in a growth medium in a container having walls; allowing said bacteria to secrete fatty acids; and collecting said fatty acids. Acid supplementation is also shown to increase productivity.

摘要翻译： 提供了包含至少一种过表达的酰基-ACP硫酯酶基因的重组细菌，并且其中至少一种来自三羧酸循环或糖酵解的基因或两者都失活。 还提供了一种生产脂肪酸的方法，所述方法包括在具有壁的容器中的生长培养基中培养包含至少一种过表达的酰基-ACP硫酯酶基因的细菌; 允许所述细菌分泌脂肪酸; 并收集所述脂肪酸。 酸补充也显示出提高生产率。

公开(公告)号：US20150259712A1

公开(公告)日：2015-09-17

申请号：US14642260

申请日：2015-03-09

申请人： William Marsh Rice University

发明人： Ka-Yiu SAN ,  SongI HAN ,  Wei LI ,  Mai LI ,  Zhilin LI

IPC分类号： C12P7/64 ,  C12N9/16 ,  C07K14/245 ,  C07K14/255

CPC分类号： C12P7/6409 ,  C07K14/245 ,  C07K14/255 ,  C12N1/38 ,  C12N9/16 ,  C12Y301/02014

摘要： Methods of improving fatty acid production in bacteria, yeast, algae, and various other microbes are presented, by either supplementing the medium with millimolar amounts of magnesium, by overexpressing a magnesium transporter, or both.

摘要翻译： 通过补充培养基中的毫摩尔量的镁，通过过表达镁转运蛋白或两者来提高细菌，酵母，藻类和各种其他微生物中脂肪酸产生的改善方法。