公开(公告)号：US20150259712A1

公开(公告)日：2015-09-17

申请号：US14642260

申请日：2015-03-09

申请人： William Marsh Rice University

发明人： Ka-Yiu SAN ,  SongI HAN ,  Wei LI ,  Mai LI ,  Zhilin LI

IPC分类号： C12P7/64 ,  C12N9/16 ,  C07K14/245 ,  C07K14/255

CPC分类号： C12P7/6409 ,  C07K14/245 ,  C07K14/255 ,  C12N1/38 ,  C12N9/16 ,  C12Y301/02014

摘要： Methods of improving fatty acid production in bacteria, yeast, algae, and various other microbes are presented, by either supplementing the medium with millimolar amounts of magnesium, by overexpressing a magnesium transporter, or both.

摘要翻译： 通过补充培养基中的毫摩尔量的镁，通过过表达镁转运蛋白或两者来提高细菌，酵母，藻类和各种其他微生物中脂肪酸产生的改善方法。

公开(公告)号：US20170009241A1

公开(公告)日：2017-01-12

申请号：US15263146

申请日：2016-09-12

申请人： William Marsh Rice University

发明人： Ka-Yiu SAN ,  George N. BENNETT ,  Hui WU

IPC分类号： C12N15/52 ,  C12N9/02 ,  C12N9/88 ,  C12N9/80 ,  C12P13/14 ,  C12P13/06 ,  C12P13/08 ,  C12P7/56 ,  C12N9/10 ,  C12N9/00

CPC分类号： C12N15/52 ,  C12N9/0008 ,  C12N9/001 ,  C12N9/0073 ,  C12N9/1085 ,  C12N9/80 ,  C12N9/88 ,  C12N9/93 ,  C12P1/04 ,  C12P7/065 ,  C12P7/18 ,  C12P7/40 ,  C12P7/46 ,  C12P7/54 ,  C12P7/56 ,  C12P13/04 ,  C12P13/06 ,  C12P13/08 ,  C12P13/14 ,  C12P13/18 ,  C12P13/222 ,  C12P13/225 ,  C12Y102/01025 ,  C12Y102/04004 ,  C12Y102/07007 ,  C12Y103/01076 ,  C12Y103/05005 ,  C12Y103/99031 ,  C12Y114/13027 ,  C12Y205/01001 ,  C12Y205/01029 ,  C12Y205/01032 ,  C12Y205/01093 ,  C12Y305/01022 ,  C12Y305/99002 ,  C12Y401/01024 ,  C12Y499/01004 ,  C12Y602/01011 ,  Y02E50/17

摘要： The disclosure relates to a metabolic transistor in microbes such as bacteria and yeast where a competitive pathway is introduced to compete with a product pathway for available carbon so as to control the carbon flux in the microbe.

摘要翻译： 本公开涉及微生物如细菌和酵母中的代谢晶体管，其中引入竞争性途径以与可用碳的产物途径竞争，以便控制微生物中的碳通量。

公开(公告)号：US20150225753A1

公开(公告)日：2015-08-13

申请号：US14607624

申请日：2015-01-28

申请人： William Marsh Rice University

发明人： Ka-Yiu SAN ,  Xixian XIE ,  Leepika TULI ,  Hui WU

IPC分类号： C12P7/64 ,  C12N9/02 ,  C07K14/195 ,  C12N9/16

CPC分类号： C12P7/6409 ,  C12N9/16 ,  C12N15/52 ,  C12P7/42 ,  C12P7/44 ,  C12Y301/02014 ,  Y02P20/52

摘要： Systems, methods and microbes that allow the biological production of hydroxy fatty acids and dicarboxylic fatty acids are provided. Specifically, hydroxy fatty acids and dicarboxylic fatty acids are produced by microbes that have been engineered to overexpress acyl ACP thioesterase plus an alkane degration pathway, such as AlkBGT or AlkJH These can be in separate microbes or the same microbe, and separate microbes can be co-cultured or sequentially cultured. Continuously fed systems transferring secreted fats from one microbial culture to another can also be used.

摘要翻译： 提供允许生物制备羟基脂肪酸和二羧酸脂肪酸的系统，方法和微生物。 具体地说，羟基脂肪酸和二羧酸脂肪酸是由已被改造过表达酰基ACP硫酯酶加上烷烃降解途径的微生物产生的，如AlkBGT或AlkJH。这些可以分开的微生物或相同的微生物，分离的微生物可以是共同的 培养或依次培养。 也可以使用将分泌的脂肪从一种微生物培养物转移到另一种微生物培养基的连续进料系统。

公开(公告)号：US20140227745A1

公开(公告)日：2014-08-14

申请号：US14176008

申请日：2014-02-07

申请人： William Marsh Rice University

发明人： Ka-Yiu SAN ,  George N. BENNETT ,  Hui WU

IPC分类号： C12N15/70 ,  C12P7/54 ,  C12P13/22 ,  C12P13/08 ,  C12P13/06 ,  C12P7/56 ,  C12P13/18

CPC分类号： C12P13/225 ,  C12N9/0008 ,  C12N9/001 ,  C12N9/0073 ,  C12N9/1085 ,  C12N9/80 ,  C12N9/88 ,  C12N9/93 ,  C12N15/52 ,  C12P1/04 ,  C12P7/065 ,  C12P7/18 ,  C12P7/40 ,  C12P7/46 ,  C12P7/54 ,  C12P7/56 ,  C12P13/04 ,  C12P13/06 ,  C12P13/08 ,  C12P13/14 ,  C12P13/18 ,  C12P13/222 ,  C12Y102/01025 ,  C12Y102/04004 ,  C12Y102/07007 ,  C12Y103/01076 ,  C12Y103/05005 ,  C12Y114/13027 ,  C12Y205/01001 ,  C12Y205/01032 ,  C12Y205/01093 ,  C12Y305/01022 ,  C12Y305/99002 ,  C12Y401/01024 ,  C12Y602/01011 ,  Y02E50/17 ,  Y02P20/52

摘要： The disclosure relates to a metabolic transistor in bacteria where a competitive pathway is introduced to compete with a product pathway for available carbon so as to control the carbon flux in the bacteria.

摘要翻译： 本公开涉及细菌中的代谢晶体管，其中引入竞争性途径以与可用碳的产物途径竞争，以便控制细菌中的碳通量。

公开(公告)号：US20190119686A1

公开(公告)日：2019-04-25

申请号：US16092042

申请日：2017-04-06

申请人： WILLIAM MARSH RICE UNIVERSITY

发明人： Ka-Yiu SAN ,  Ping LIU ,  Sha LI

IPC分类号： C12N15/52 ,  C12N1/20 ,  C12P1/04 ,  C12N15/70 ,  C12N9/12 ,  C12N15/74 ,  C12P7/64

摘要： The present disclosure describes a genetically engineered bacteria that relieves the catabolite repression problem exerted by the Spot 42 small regulatory RNA by adding a galactokinase that does not contain the Spot 42 binding region. As such, galK and galM and the like can be expressed allow better galactose utilization.

公开(公告)号：US20180371508A1

公开(公告)日：2018-12-27

申请号：US16015101

申请日：2018-06-21

申请人： WILLIAM MARSH RICE UNIVERSITY

发明人： Ka-Yiu SAN ,  George N. Bennett ,  Irene Martinez

IPC分类号： C12P7/46

CPC分类号： C12P7/46 ,  C12N9/88 ,  C12Y402/01002 ,  C12Y604/01001

摘要： Microbes and methods used to convert renewable carbon sources such as glucose, sucrose, biomass hydrolysate, methanol or formate, to succinate-derived products, such as fumarate or malate, which are desirable products having many uses.

公开(公告)号：US20160040171A1

公开(公告)日：2016-02-11

申请号：US14919911

申请日：2015-10-22

申请人： WILLIAM MARSH RICE UNIVERSITY ,  TECHNOLOGY HOLDING LLC

发明人： Ka-Yiu SAN ,  George BENNETT ,  Mukund KARANJIKAR

IPC分类号： C12N15/52 ,  C12P5/00 ,  C12P7/62 ,  C12P7/64

CPC分类号： C12N15/52 ,  C12M29/18 ,  C12M47/10 ,  C12P5/00 ,  C12P5/02 ,  C12P7/40 ,  C12P7/62 ,  C12P7/6409

摘要： The disclosure relates to biological methods of making a hydrocarbon feedstock wherein one-carbon substrates are converted into useful chemicals and fuels. Particularly, genetically engineered bacteria are used to make C4-C10 fatty acids or derivatives from one-carbon substrates such as methanol and carbon dioxide.

摘要翻译： 本公开涉及制备烃原料的生物学方法，其中将一碳底物转化为有用的化学物质和燃料。 特别地，遗传工程细菌用于制备C4-C10脂肪酸或一碳底物如甲醇和二氧化碳的衍生物。

公开(公告)号：US20190144898A1

公开(公告)日：2019-05-16

申请号：US16098914

申请日：2017-05-05

申请人： William Marsh Rice University

发明人： Ka-Yiu SAN ,  Zhilin LI ,  Xian ZHANG

IPC分类号： C12P7/64 ,  C12N15/74 ,  C12N1/20 ,  C12N15/52 ,  C12N9/02

摘要： This invention describes a method of using microbial to produce fats, such as fatty acids and their derivatives, or products derived from the fatty acid synthesis cycle, such as hydroxyfatty acids, methyl ketones, and the like.

公开(公告)号：US20140193867A1

公开(公告)日：2014-07-10

申请号：US14104628

申请日：2013-12-12

申请人： William Marsh Rice University

发明人： Ka-Yiu SAN ,  Hui WU

IPC分类号： C12P7/64

CPC分类号： C12P7/6409 ,  C12N9/1029 ,  C12N9/93 ,  C12Y203/0118 ,  C12Y602/01017

摘要： Engineered bacteria that produce a predominance of odd-chain free fatty acids.

摘要翻译： 产生奇异链游离脂肪酸优势的工程细菌。

公开(公告)号：US20130084600A1

公开(公告)日：2013-04-04

申请号：US13680608

申请日：2012-11-19

申请人： WILLIAM MARSH RICE UNIVERSITY

发明人： Ka-Yiu SAN ,  George N. BENNETT ,  Yipeng WANG

IPC分类号： C12N15/70

CPC分类号： C12N15/70 ,  C12N9/0008 ,  C12N9/1205 ,  C12P5/007 ,  C12P7/52 ,  C12P17/08 ,  C12P19/36 ,  C12Y101/01008 ,  C12Y101/01094 ,  C12Y207/01023

摘要： This invention is metabolically engineer bacterial strains that provide increased intracellular NADPH availability for the purpose of increasing the yield and productivity of NADPH-dependent compounds. In the invention, native NAD-dependent GAPDH is replaced with NADP-dependent GAPDH plus overexpressed NADK. Uses for the bacteria are also provided.

摘要翻译： 本发明是为提高NADPH依赖化合物的产量和生产力而提供增加的细胞内NADPH可用性的代谢工程菌株。 在本发明中，天然NAD依赖性GAPDH被NADP依赖性GAPDH加上过表达的NADK替代。 也提供了细菌的使用。