公开(公告)号：US07262270B2

公开(公告)日：2007-08-28

申请号：US10655667

申请日：2003-09-05

申请人： Winfried Weissenhorn ,  David Prashker, legal representative ,  Nicholas Mantis ,  Marian R. Neutra ,  Pamela Kozlowski

发明人： Don Wiley, deceased

IPC分类号： A61K38/00 ,  A61K39/00 ,  A61K39/12 ,  C07K1/00

CPC分类号： C07K14/005 ,  A61K2039/54 ,  A61K2039/542 ,  A61K2039/545 ,  C07K2319/00 ,  C12N2740/16122 ,  C12N2760/16022 ,  Y10S530/826

摘要： The present invention provides a fusion protein construct (gp41HA) consisting of the ectodomain of the HIV-1IIIB envelope glycoprotein gp41 fused to a fragment of the influenza virus HA2 hemagglutinin protein. Immunization in-vivo via an intraperitoneal prime followed by intranasal or intragastric boosts with gp41HA induces high concentrations of serum IgG antibodies and fecal IgA antibodies that reacted with gp41 in HIV-1IIIB viral lysate and are cross-reactive with gp41 in HIV-1MN lysate. Followup analyses by indirect immunofluorescence showed that both serum IgG and fecal IgA recognized human peripheral blood mononuclear cells infected with either syncytium-inducing (SI) or non-syncytium-inducing (NSI) North American HIV-1 field isolates, but not uninfected cells.

摘要翻译： 本发明提供了融合蛋白质构建体（gp41HA），其由与流感病毒HA2血凝素蛋白片段融合的HIV-1 IIIB包膜糖蛋白gp41的胞外域组成。 通过腹膜内注射进行体内免疫，随后用gp41HA进行鼻内或胃内加压，诱导高浓度的血清IgG抗体和与HIV-1 IIIB III病毒裂解物中的gp41反应的血清IgG抗体和粪便IgA抗体，并且是交叉反应性 在HIV-1 MN溶解产物中具有gp41。 通过间接免疫荧光进行的随访分析显示，血清IgG和粪便IgA均识别出感染合胞体诱导（SI）或非合胞体诱导（NSI）北美HIV-1场分离株但不是未感染细胞的人外周血单核细胞。