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1.发明申请Genetically Modified Microorganisms Capable of Producing Beta-Glucans and Methods for Producing Beta-Glucans 有权能够生产β-葡聚糖的转基因微生物和生产β-葡聚糖的方法公开(公告)号：US20140011243A1
公开(公告)日：2014-01-09
申请号：US13935043
申请日：2013-07-03
申请人： Wintershall Holding GmbH , BASF SE
发明人： Beata Brockmann , Andrea Herold , Oskar Zelder , Stefan Haefner , Christian Fleck , Hartwig Schröder , Mari Granström , Julia Kristiane Schmidt
CPC分类号： C12P19/04 , C08B37/0024 , C08L5/00 , C12N9/1051 , C12N15/80 , C12P19/18 , C12Y204/01034 , Y02P20/52
摘要： The present invention relates to genetically modified microorganisms capable of producing beta-glucans, characterized in that said genetically modified microorganism overexpresses (i) a polynucleotide encoding a polypeptide having 1,3-β-D-glucan synthase-activity, and/or (ii) a polypeptide having 1,3-β-D-glucan synthase-activity, compared to a corresponding non-modified control microorganism of the same strain. The present invention also relates to the use of a polynucleotide encoding a polypeptide having 1,3-β-D-glucan synthase-activity or the use of such a polypeptide for producing β-glucans. Furthermore, the present invention relates to methods for producing β-glucans comprising the introduction of a promoter upstream of a polynucleotide encoding a polypeptide having 1,3-β-D-glucan synthase-activity thereby increasing the expression of said polynucleotide, or a polynucleotide encoding a polypeptide having 1,3-β-D-glucan synthase-activity into a microorganism being able to synthesize β-glucans.
摘要翻译： 本发明涉及能够产生β-葡聚糖的转基因微生物，其特征在于所述遗传修饰的微生物过表达（i）编码具有1,3-β-D-葡聚糖合酶活性的多肽的多核苷酸和/或（ii ）具有1,3-β-D-葡聚糖合酶活性的多肽，与相同菌株的相应未修饰的对照微生物相比较。 本发明还涉及编码具有1,3-β-D-葡聚糖合酶活性的多肽的多核苷酸的用途或这种多肽用于产生β-葡聚糖的用途。 此外，本发明涉及生产β-葡聚糖的方法，包括在编码具有1,3-β-D-葡聚糖合酶活性的多肽的多核苷酸上游引入启动子，从而增加所述多核苷酸的表达，或多核苷酸 将具有1,3-β-D-葡聚糖合酶活性的多肽编码成能够合成β-葡聚糖的微生物。
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2.发明申请GENETICALLY MODIFIED MICROORGANISMS CAPABLE OF PRODUCING BETA-GLUCANS AND METHODS FOR PRODUCING BETA-GLUCANS 有权能够生产β-葡聚糖的基因改造的微生物和用于生产β-葡聚糖的方法公开(公告)号：US20160194676A1
公开(公告)日：2016-07-07
申请号：US15074330
申请日：2016-03-18
申请人： BASF SE , Wintershall Holding GmbH
发明人： Beata Brockmann , Andrea Herold , Oskar Zelder , Stefan Haefner , Christian Fleck , Hartwig Schröder , Mari Granström , Julia Kristiane Schmidt
CPC分类号： C12P19/04 , C08B37/0024 , C08L5/00 , C12N9/1051 , C12N15/80 , C12P19/18 , C12Y204/01034 , Y02P20/52
摘要： The present invention relates to genetically modified microorganisms capable of producing beta-glucans, characterized in that the genetically modified microorganism overexpresses (i) a polynucleotide encoding a polypeptide having 1,3-β-D-glucan synthase-activity, and/or (ii) a polypeptide having 1,3-β-D-glucan synthase-activity, compared to a corresponding non-modified control microorganism of the same strain. The present invention also relates to the use of a polynucleotide encoding a polypeptide having 1,3-β-D-glucan synthase-activity or the use of such a polypeptide for producing β-glucans. Furthermore, the present invention relates to methods for producing β-glucans comprising the introduction of a promoter upstream of a polynucleotide encoding a polypeptide having 1,3-β-D-glucan synthase-activity thereby increasing the expression of the polynucleotide, or a polynucleotide encoding a polypeptide having 1,3-β-D-glucan synthase-activity into a microorganism being able to synthesize β-glucans.
摘要翻译： 本发明涉及能够产生β-葡聚糖的转基因微生物，其特征在于，遗传修饰的微生物过表达（i）编码具有1,3-葡聚糖合酶活性的多肽的多核苷酸和/或（ ii）与相同菌株的相应未修饰的对照微生物相比，具有1,3-葡聚糖 - D-葡聚糖合酶活性的多肽。 本发明还涉及编码具有1,3-葡萄糖 - D-葡聚糖合酶活性的多肽的多核苷酸的用途或这种多肽用于生产葡聚糖的用途。 此外，本发明涉及生产葡聚糖的方法，包括在编码具有1,3-葡萄糖-D-葡聚糖合酶活性的多肽的多核苷酸上游引入启动子，从而增加多核苷酸的表达，或 编码具有1,3-葡聚糖合酶活性的多肽的多核苷酸能够合成葡聚糖。
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3.发明授权Genetically modified microorganisms capable of producing beta-glucans and methods for producing beta-glucans 有权能够产生β-葡聚糖的转基因微生物和生产β-葡聚糖的方法公开(公告)号：US09322041B2
公开(公告)日：2016-04-26
申请号：US13935043
申请日：2013-07-03
申请人： BASF SE , Wintershall Holding GmbH
发明人： Beata Brockmann , Andrea Herold , Oskar Zelder , Stefan Haefner , Christian Fleck , Hartwig Schröder , Mari Granström , Julia Kristiane Schmidt
CPC分类号： C12P19/04 , C08B37/0024 , C08L5/00 , C12N9/1051 , C12N15/80 , C12P19/18 , C12Y204/01034 , Y02P20/52
摘要： The present invention relates to genetically modified microorganisms capable of producing beta-glucans, characterized in that the genetically modified microorganism overexpresses (i) a polynucleotide encoding a polypeptide having 1,3-β-D-glucan synthase-activity, and/or (ii) a polypeptide having 1,3-β-D-glucan synthase-activity, compared to a corresponding non-modified control microorganism of the same strain. The present invention also relates to the use of a polynucleotide encoding a polypeptide having 1,3-β-D-glucan synthase-activity or the use of such a polypeptide for producing β-glucans. Furthermore, the present invention relates to methods for producing β-glucans comprising the introduction of a promoter upstream of a polynucleotide encoding a polypeptide having 1,3-β-D-glucan synthase-activity thereby increasing the expression of the polynucleotide, or a polynucleotide encoding a polypeptide having 1,3-β-D-glucan synthase-activity into a microorganism being able to synthesize β-glucans.
摘要翻译： 本发明涉及能够产生β-葡聚糖的转基因微生物，其特征在于，遗传修饰的微生物过表达（i）编码具有1,3-葡聚糖合酶活性的多肽的多核苷酸和/或（ ii）与相同菌株的相应未修饰的对照微生物相比，具有1,3-葡聚糖 - D-葡聚糖合酶活性的多肽。 本发明还涉及编码具有1,3-葡萄糖 - D-葡聚糖合酶活性的多肽的多核苷酸的用途或这种多肽用于生产葡聚糖的用途。 此外，本发明涉及生产葡聚糖的方法，包括在编码具有1,3-葡萄糖-D-葡聚糖合酶活性的多肽的多核苷酸上游引入启动子，从而增加多核苷酸的表达，或 编码具有1,3-葡聚糖合酶活性的多肽的多核苷酸能够合成葡聚糖。
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4.发明授权Genetically modified microorganisms capable of producing β-glucans and methods for producing β-glucans 有权能够生产葡聚糖的转基因微生物和生产葡聚糖的方法公开(公告)号：US09388441B1
公开(公告)日：2016-07-12
申请号：US15074330
申请日：2016-03-18
申请人： BASF SE , Wintershall Holding GmbH
发明人： Beata Brockmann , Andrea Herold , Oskar Zelder , Stefan Haefner , Christian Fleck , Hartwig Schröder , Mari Granström , Julia Kristiane Schmidt
CPC分类号： C12P19/04 , C08B37/0024 , C08L5/00 , C12N9/1051 , C12N15/80 , C12P19/18 , C12Y204/01034 , Y02P20/52
摘要： The present invention relates to genetically modified microorganisms capable of producing beta-glucans, characterized in that the genetically modified microorganism overexpresses (i) a polynucleotide encoding a polypeptide having 1,3-β-D-glucan synthase-activity, and/or (ii) a polypeptide having 1,3-β-D-glucan synthase-activity, compared to a corresponding non-modified control microorganism of the same strain. The present invention also relates to the use of a polynucleotide encoding a polypeptide having 1,3-β-D-glucan synthase-activity or the use of such a polypeptide for producing β-glucans. Furthermore, the present invention relates to methods for producing β-glucans comprising the introduction of a promoter upstream of a polynucleotide encoding a polypeptide having 1,3-β-D-glucan synthase-activity thereby increasing the expression of the polynucleotide, or a polynucleotide encoding a polypeptide having 1,3-β-D-glucan synthase-activity into a microorganism being able to synthesize β-glucans.
摘要翻译： 本发明涉及能够产生β-葡聚糖的转基因微生物，其特征在于，遗传修饰的微生物过表达（i）编码具有1,3-葡聚糖合酶活性的多肽的多核苷酸和/或（ ii）与相同菌株的相应未修饰的对照微生物相比，具有1,3-葡聚糖 - D-葡聚糖合酶活性的多肽。 本发明还涉及编码具有1,3-葡萄糖 - D-葡聚糖合酶活性的多肽的多核苷酸的用途或这种多肽用于生产葡聚糖的用途。 此外，本发明涉及生产葡聚糖的方法，包括在编码具有1,3-葡萄糖-D-葡聚糖合酶活性的多肽的多核苷酸上游引入启动子，从而增加多核苷酸的表达，或 编码具有1,3-葡聚糖合酶活性的多肽的多核苷酸能够合成葡聚糖。
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公开(公告)号：US20130310553A1
公开(公告)日：2013-11-21
申请号：US13893532
申请日：2013-05-14
发明人： Tobias Käppler , Thorsten Haas , Julia Kristiane Schmidt , Christian Fleck , Stephan Freyer , Robert Bayer
IPC分类号： C08B37/00
CPC分类号： C08B37/0024 , C08L5/00
摘要： The present invention relates to novel methods for precipitating beta-glucan (β-glucan) by using high-molecular polyethylene glycol (PEG) and re-dissolving the precipitated β-glucan in a suitable medium. The novel method of the present invention may also include drying the precipitated β-glucan and/or swelling the precipitated b-glucan in a suitable solution before re-dissolving the β-glucan.
摘要翻译： 本发明涉及通过使用高分子量聚乙二醇（PEG）和将沉淀的β-葡聚糖再溶解在合适的介质中来沉淀β-葡聚糖（β-葡聚糖）的新方法。 本发明的新方法还可以包括在重新溶解β-葡聚糖之前干燥沉淀的β-葡聚糖和/或在合适的溶液中溶胀沉淀的β-葡聚糖。
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