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公开(公告)号：US20180201972A1
公开(公告)日：2018-07-19
申请号：US15918357
申请日：2018-03-12
Applicant: Wisconsin Alumni Research Foundation
Inventor： William L. Murphy , Siyoung Choi , Xiaohua Yu
Abstract: Disclosed are methods for cell transfection and regulating cellular behavior. More particularly, the present disclosure relates to methods of non-viral cell transfection and regulating cellular behavior using mineral coatings that allow for the enhanced transfection of cells. The mineral coatings bind polynucleotides and provide a source of calcium and phosphate ions to enhance transfection. The present disclosure also provides a high throughput platform for screening non-viral transfection of cells. The methods of the present disclosure also provide an advantageous polynucleotide delivery platform because the mineral coatings may be deposited on various medical device materials after being specifically developed using the high throughput screening platform.
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公开(公告)号：US09951374B2
公开(公告)日：2018-04-24
申请号：US13769993
申请日：2013-02-19
Applicant: Wisconsin Alumni Research Foundation
Inventor： William L. Murphy , Siyoung Choi , Xiaohua Yu
Abstract: Disclosed are methods for cell transfection and regulating cellular behavior. More particularly, the present disclosure relates to methods of non-viral cell transfection and regulating cellular behavior using mineral coatings that allow for the enhanced transfection of cells. The mineral coatings bind polynucleotides and provide a source of calcium and phosphate ions to enhance transfection. The present disclosure also provides a high throughput platform for screening non-viral transfection of cells. The methods of the present disclosure also provide an advantageous polynucleotide delivery platform because the mineral coatings may be deposited on various medical device materials after being specifically developed using the high throughput screening platform.
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3.发明申请公开(公告)号：US20140235498A1
公开(公告)日：2014-08-21
申请号：US13769993
申请日：2013-02-19
Applicant: WISCONSIN ALUMNI RESEARCH FOUNDATION
Inventor： William L. Murphy , Siyoung Choi , Xiaohua Yu
Abstract: Disclosed are methods for cell transfection and regulating cellular behavior. More particularly, the present disclosure relates to methods of non-viral cell transfection and regulating cellular behavior using mineral coatings that allow for the enhanced transfection of cells. The mineral coatings bind polynucleotides and provide a source of calcium and phosphate ions to enhance transfection. The present disclosure also provides a high throughput platform for screening non-viral transfection of cells. The methods of the present disclosure also provide an advantageous polynucleotide delivery platform because the mineral coatings may be deposited on various medical device materials after being specifically developed using the high throughput screening platform.
Abstract translation: 公开了细胞转染和调节细胞行为的方法。 更具体地说，本公开内容涉及非病毒细胞转染的方法，并且使用允许增强细胞转染的矿物涂层调节细胞行为。 矿物涂层结合多核苷酸并提供钙和磷酸根离子来增强转染。 本公开还提供用于筛选细胞的非病毒转染的高通量平台。 本公开的方法还提供有利的多核苷酸递送平台，因为矿物涂层可以在使用高通量筛选平台进行专门开发之后沉积在各种医疗装置材料上。
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公开(公告)号：US10745739B2
公开(公告)日：2020-08-18
申请号：US15918357
申请日：2018-03-12
Applicant: Wisconsin Alumni Research Foundation
Inventor： William L. Murphy , Siyoung Choi , Xiaohua Yu
Abstract: Disclosed are methods for cell transfection and regulating cellular behavior. More particularly, the present disclosure relates to methods of non-viral cell transfection and regulating cellular behavior using mineral coatings that allow for the enhanced transfection of cells. The mineral coatings bind polynucleotides and provide a source of calcium and phosphate ions to enhance transfection. The present disclosure also provides a high throughput platform for screening non-viral transfection of cells. The methods of the present disclosure also provide an advantageous polynucleotide delivery platform because the mineral coatings may be deposited on various medical device materials after being specifically developed using the high throughput screening platform.
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