公开(公告)号：US20250127921A1

公开(公告)日：2025-04-24

申请号：US18682418

申请日：2022-08-09

Applicant: Wisconsin Alumni Research Foundation

Inventor： Shaoqin Gong ,  Ruosen Xie ,  Xiuxiu Wang

IPC: A61K47/69 ,  A61K9/51 ,  A61K31/7088 ,  A61K38/46 ,  A61K47/54 ,  C12N9/22 ,  C12N15/11 ,  C12N15/88

Abstract: Provided herein are self-assembled nanoparticles (NPs), pharmaceutical compositions containing such NPs and methods of using such NPs. The NPs comprise an amphiphilic copolymer and a ribonucleoprotein (RNP), and optionally ssODN, wherein: the amphiphilic copolymer is a water-soluble block copolymer comprising a poly(C2-3 alkylene glycol) block and an acrylic block comprising a poly(acrylate), poly(methacrylate) or poly(acrylate/methacrylate) block; the acrylic block comprise ester side chains bearing substituted or unsubstituted alkylamine groups; and the RNP, ssODN, and the acrylic block of the amphiphilic copolymer form a core of the self-assembled nanoparticle, and the poly(ethylene glycol) block of the amphiphilic copolymer forms the exterior of the self-assembled nanoparticle.