公开(公告)号：US20080193927A1

公开(公告)日：2008-08-14

申请号：US11631986

申请日：2005-07-27

申请人： Wofgang Mann ,  Christoph Gauer

发明人： Wofgang Mann ,  Christoph Gauer

IPC分类号： C12Q1/68 ,  C12M1/34

CPC分类号： C12Q1/6827 ,  C12Q1/6851 ,  C12Q2545/114 ,  C12Q2537/143

摘要： The invention relates to a method for determining the abundance of a given sequence or several sequences identical or nearly identical to the given sequence in a sample. The method comprises the following steps: carrying out one or more amplification reactions by means of which several different sections of the sequence or sequences of the sample can be amplified to give an amplified product, detection of whether given different sections of the sequence in the sample have been amplified and determination of the number of the sequence(s) in the sample by means of the abundance of the presence or otherwise of the given different sections in the amplified product.

摘要翻译： 本发明涉及一种用于确定给定序列的丰度或与样品中给定序列相同或几乎相同的几个序列的方法。 该方法包括以下步骤：进行一个或多个扩增反应，通过其可以扩增样品的序列或序列的几个不同部分以产生扩增产物，检测样品中是否给予序列的不同切片 已经通过扩增产物中给定的不同切片的存在或其它的丰度来扩增和确定样品中序列的数目。