公开(公告)号：US09739700B2

公开(公告)日：2017-08-22

申请号：US14434914

申请日：2013-09-03

Applicant: XIAMEN UNIVERSITY

Inventor： Xiaomei Yan ,  Shaobin Zhu

IPC: G01N15/14 ,  G01N21/85

CPC classification number: G01N15/1434 ,  G01N15/1459 ,  G01N21/53 ,  G01N21/85 ,  G01N2015/0038 ,  G01N2015/1413 ,  G01N2015/1447 ,  G01N2201/06113

Abstract: Disclosed is a method for detecting nano-particles, comprising the steps of (1) compressing a sample liquid to be tested into a sample liquid flow by hydrodynamic focusing using a sheath fluid; (2) irradiating a measuring light to the sample liquid flow, wherein a single nano-particle in the sample liquid flow is irradiated by the measuring light for a duration of 0.1-10 milliseconds; (3) defining the area in which the measuring light irradiates the sample liquid flow as a detecting area, and collecting light signals emitted from the area irradiated by the measuring light by a lens imaging system, and allowing the light signals collected by the lens imaging system to pass a field stop, so as to filter out the light signals outside the detecting area and enrich the light signals from the detecting area; and (4) subjecting the light signals enriched by the field stop to optoelectronic signal conversion. The method can achieve detection for nano-particles with a low refractive index and a particle size of 24-1000 nm as well as nano-scale gold particles with a particle size of 6.7-150 nm.

公开(公告)号：US20150233812A1

公开(公告)日：2015-08-20

申请号：US14434914

申请日：2013-09-03

Applicant: XIAMEN UNIVERSITY

Inventor： Xiaomei Yan ,  Shaobin Zhu

IPC: G01N15/14 ,  G01N21/85

CPC classification number: G01N15/1434 ,  G01N15/1459 ,  G01N21/53 ,  G01N21/85 ,  G01N2015/0038 ,  G01N2015/1413 ,  G01N2015/1447 ,  G01N2201/06113

Abstract: Disclosed is a method for detecting nano-particles, comprising the steps of (1) compressing a sample liquid to be tested into a sample liquid flow by hydrodynamic focusing using a sheath fluid; (2) irradiating a measuring light to the sample liquid flow, wherein a single nano-particle in the sample liquid flow is irradiated by the measuring light for a duration of 0.1-10 milliseconds; (3) defining the area in which the measuring light irradiates the sample liquid flow as a detecting area, and collecting light signals emitted from the area irradiated by the measuring light by a lens imaging system, and allowing the light signals collected by the lens imaging system to pass a field stop, so as to filter out the light signals outside the detecting area and enrich the light signals from the detecting area; and (4) subjecting the light signals enriched by the field stop to optoelectronic signal conversion. The method can achieve detection for nano-particles with a low refractive index and a particle size of 24-1000 nm as well as nano-scale gold particles with a particle size of 6.7-150 nm.

Abstract translation: 公开了一种用于检测纳米颗粒的方法，包括以下步骤：（1）使用鞘液通过流体动力聚焦将待测样品液压缩成样品液流; （2）向样品液体流照射测量光，其中样品液体流中的单个纳米颗粒被测量光照射持续0.1-10毫秒; （3）限定测量光照射样品液流的区域作为检测区域，并且通过透镜成像系统收集从测量光照射的区域发射的光信号，并且允许由透镜成像收集的光信号 系统通过场停止，以便滤除检测区域外的光信号，并丰富来自检测区域的光信号; 和（4）使由场停止富集的光信号进行光电信号转换。 该方法可以实现具有低折射率和粒径为24-1000nm的纳米颗粒以及粒径为6.7-150nm的纳米级金颗粒的检测。