公开(公告)号：US20230295751A1

公开(公告)日：2023-09-21

申请号：US18185344

申请日：2023-03-16

申请人： Xudong Cao

发明人： Xingkai Hao ,  Xudong Cao

IPC分类号： C12Q1/70 ,  C12Q1/682 ,  B01L3/00

CPC分类号： C12Q1/701 ,  C12Q1/682 ,  B01L3/502761 ,  C12Q1/6804

摘要： The invention discloses a biosensor chip and a COVID-19 test kit. The COVID-19 test kit includes a biosensor chip, rolling circle amplification (RCA) primers, RCA padlock probes, and ssDNA conjugated nanoparticles (i.e., ssDNA-NPs probe). In this invention, the surface of the biosensor chip is modified with functional polymers; subsequently, detection ligands are grafted on the modified sensor chip surface to capture the targets. The developed biosensor chip achieves rapid and ultra-sensitive on-site detection of COVID-19 infections. In addition, a signal amplification method is employed using RCA products hybridized ssDNA-NPs probes (i.e., RCA-NPs) complex to amplify the detection signal and to improve the detection sensitivity. The RCA sequence in this invention is designed to generate tandem repeating aptamers and ssDNA-NPs probe hybridization sites so that multiple ssDNA-NPs probes can be hybridized with the RCA products and attached to the captured targets in order to increase the target mass, resulting in detection signal intensification.

公开(公告)号：US20140047734A1

公开(公告)日：2014-02-20

申请号：US13995495

申请日：2012-11-09

申请人： Xudong Cao ,  Seong Cho Lee ,  Dongdong Yin ,  Kun Li ,  Xinxiao Hua ,  Lifeng Wu ,  Chao Li ,  Wenxu Wang

发明人： Xudong Cao ,  Seong Cho Lee ,  Dongdong Yin ,  Kun Li ,  Xinxiao Hua ,  Lifeng Wu ,  Chao Li ,  Wenxu Wang

IPC分类号： F26B21/06 ,  F26B25/06

CPC分类号： F26B25/06 ,  F26B21/06 ,  G03F7/38

摘要： A prebake equipment for drying a substrate (105) and an air discharge method, the prebake equipment comprising: a bottom plate (105), a periphery frame (101) and an upper cover plate, the bottom plate (105) and the upper cover plate being spaced from each other, the periphery frame (101) being provided between the bottom plate (105) and the upper cover plate such that the bottom plate (105), the periphery frame (101) and the upper cover plate define a cavity, wherein discharge holes are provided at the periphery frame (101) and a filler (104) for filtering moisture and dust in the air stream is provided between the bottom plate (105) and the periphery frame (101).

摘要翻译： 一种用于干燥基材（105）的预烘设备和排气方法，所述预烘烤设备包括：底板（105），周边框架（101）和上盖板，所述底板（105）和所述上盖 平板彼此间隔开，所述周边框架（101）设置在所述底板（105）和所述上盖板之间，使得所述底板（105），所述周边框架（101）和所述上盖板限定空腔 其特征在于，在所述周缘框架（101）设置有排出孔，在所述底板（105）与所述周边框架（101）之间设置有用于过滤空气流中的水分和灰尘的填料（104）。