公开(公告)号：US20140066490A1

公开(公告)日：2014-03-06

申请号：US13966337

申请日：2013-08-14

Applicant: YEDA Research and Development Co. Ltd.

Inventor： Ehud Y. SHAPIRO ,  Maya KANAN-HANUM ,  Yehonatan DOUEK ,  Rivka ADAR

IPC: C12N15/113 ,  C12N15/10

CPC classification number: C12N15/1136 ,  B82Y10/00 ,  C12N15/1089 ,  C12N15/111 ,  C12N15/1135 ,  C12N2310/127 ,  C12N2310/3519 ,  C12N2310/531 ,  C12N2320/13 ,  C12N2320/50 ,  C12N2330/31 ,  G06N3/123 ,  G16B50/00

Abstract: A system and method for programming DNAzymes to be utilized as programmable drugs, which are active only in the presence of specific input combinations and/or certain conditions.

Abstract translation: 用于编程用作可编程药物的DNA酶的系统和方法，其仅在存在特定输入组合和/或某些条件时是有效的。

公开(公告)号：US20150252362A1

公开(公告)日：2015-09-10

申请号：US14607080

申请日：2015-01-28

Applicant: YEDA RESEARCH AND DEVELOPMENT CO. LTD.

Inventor： Ehud Y. SHAPIRO ,  Gregory LINSHIZ ,  Ilan GRONAU ,  Rivka ADAR ,  Shai KAPLAN ,  Sivan TUVI

IPC: C12N15/10 ,  G06F19/22

CPC classification number: C12N15/1089 ,  C12N15/1031 ,  C12N15/66 ,  C12Q1/686 ,  G16B30/00 ,  G16B35/00 ,  G16C20/60

Abstract: A method for manufacturing synthetic genes and combinatorial DNA and protein libraries, termed here Divide and Conquer-DNA synthesis (D&C-DNA synthesis) method. The method can be used in a systematic and automated way to synthesize any long DNA molecule and, more generally, any combinatorial molecular library having the mathematical property of being a regular set of strings. The D&C-DNA synthesis method is an algorithm design paradigm that works by recursively breaking down a problem into two or more sub-problems of the same type. The division of long DNA sequences is done in silico. The assembly of the sequence is done in vitro. The D&C-DNA synthesis method protocol consists of a tree, in which each node represents an intermediate sequence. The internal nodes are created in elongation reactions from their daughter nodes, and the leaves are synthesized directly. After each elongation only one DNA strand passes to the next level in the tree until receiving the final product. Optionally and preferably, error correction is performed to correct any errors which may have occurred during the synthetic process.

Abstract translation: 用于制造合成基因和组合DNA和蛋白质文库的方法，这里称为Div和Conquer-DNA合成（D＆C-DNA合成）方法。 该方法可以以系统和自动的方式用于合成任何长的DNA分子，更一般地，任何组合分子文库具有作为一组规则的串的数学性质。 D＆C-DNA合成方法是一种算法设计范例，通过递归地将问题分解成两个或更多个相同类型的子问题。 长DNA序列的划分是在电脑中完成的。 序列的组装在体外进行。 D＆C-DNA合成方法协议由一棵树组成，其中每个节点表示中间序列。 内部节点是在其子节点的伸长反应中产生的，叶片直接合成。 在每次延伸之后，只有一条DNA链在树中传递到下一个级别，直到接收到最终产物。 可选地，优选地，执行错误校正以校正在合成过程中可能发生的任何错误。