公开(公告)号：US20240084025A1

公开(公告)日：2024-03-14

申请号：US18273888

申请日：2022-01-21

申请人： YUHAN CORPORATION

发明人： Ju-Young PARK ,  Sung-Hyun CHOI ,  Yun-Hee CHOI ,  Won-Tae KIM

IPC分类号： C07K16/28 ,  C07K1/22 ,  C07K1/34 ,  C07K16/32

CPC分类号： C07K16/2878 ,  C07K1/22 ,  C07K1/34 ,  C07K16/32 ,  C07K2317/31 ,  C07K2317/56

摘要： Provided is a method for purifying an anti-4-1BB/anti-HER2 bispecific antibody, the method of which includes carrying out affinity chromatography with a sodium acetate buffer containing a certain inorganic salt as an elution buffer. The purification method of the presently claimed subject matter increases the elution of an antibody in the intact form, thereby being able to provide an anti-4-1BB/anti-HER2 bispecific antibody in the intact form in high purity and high yield.