公开(公告)号：US20120264112A1

公开(公告)日：2012-10-18

申请号：US13456330

申请日：2012-04-26

申请人： Yasushi YAMAZOE ,  Kiyoshi NAGATA

发明人： Yasushi YAMAZOE ,  Kiyoshi NAGATA

IPC分类号： C12Q1/70 ,  C12N5/10

CPC分类号： C12Q1/6897 ,  C12Q1/02 ,  C12Q1/26 ,  G01N33/5008 ,  G01N33/5023 ,  G01N33/5067 ,  G01N2333/90245 ,  G01N2333/90251 ,  G01N2500/04 ,  G01N2500/10

摘要： A method for measuring human CYP3A inducibility upon administration of a test drug, characterized in that a non-human animal to which a test drug is administered or a population of human cells cultured in a medium containing a test drug is infected with viruses (A) and (B); virus (A) being an adenovirus which is used as a vector and engineered by incorporating thereto a detectable reporter gene and at least 3 human PXR binding regions falling within an untranslated region of a human CYP3A gene, and virus (B) being an adenovirus which is used as a vector and engineered by incorporating thereto a human PXR cDNA; and subsequently expression level of the reporter gene is determined in the non-human animal or the cultured human cells.

摘要翻译： 一种在给药试验药物时测量人CYP3A诱导率的方法，其特征在于，在含有试验药物的培养基中培养的非人动物或受试药物培养的人群受病毒感染（A） 和（B）; 病毒（A）是腺病毒，其用作载体并通过掺入可检测的报道基因和位于人CYP3A基因的非翻译区内的至少3个人PXR结合区域而工程化，并且病毒（B）是腺病毒， 用作载体并通过掺入人PXR cDNA而工程化; 并且随后在非人动物或培养的人细胞中测定报道基因的表达水平。

公开(公告)号：US20090029351A1

公开(公告)日：2009-01-29

申请号：US12180822

申请日：2008-07-28

申请人： Yasushi YAMAZOE ,  Kiyoshi Nagata

发明人： Yasushi YAMAZOE ,  Kiyoshi Nagata

IPC分类号： C12Q1/70 ,  C12Q1/68

CPC分类号： C12Q1/6897 ,  C12Q1/02 ,  C12Q1/26 ,  G01N33/5008 ,  G01N33/5023 ,  G01N33/5067 ,  G01N2333/90245 ,  G01N2333/90251 ,  G01N2500/04 ,  G01N2500/10

摘要： A method for measuring human CYP3A inducibility upon administration of a test drug, characterized in that a non-human animal to which a test drug is administered or a population of human cells cultured in a medium containing a test drug is infected with viruses (A) and (B); virus (A) being an adenovirus which is used as a vector and engineered by incorporating thereto a detectable reporter gene and at least 3 human PXR binding regions falling within an untranslated region of a human CYP3A gene, and virus (B) being an adenovirus which is used as a vector and engineered by incorporating thereto a human PXR cDNA; and subsequently expression level of the reporter gene is determined in the non-human animal or the cultured human cells.The present invention ensures convenient and accurate evaluation of human CYP3A inducibility upon administration of a test drug to a human subject, providing accurate evaluation in terms of the efficacy of the test drug, occurrence of side effects, disappearance of the drug effect, etc.

摘要翻译： 一种在给药试验药物时测定人CYP3A诱导率的方法，其特征在于，在含有试验药物的培养基中培养的非人动物或受试药物培养的人群受病毒感染（A） 和（B）; 病毒（A）是腺病毒，其用作载体并通过掺入可检测的报道基因和位于人CYP3A基因的非翻译区内的至少3个人PXR结合区域而工程化，并且病毒（B）是腺病毒， 用作载体并通过掺入人PXR cDNA而工程化; 并且随后在非人动物或培养的人细胞中测定报道基因的表达水平。 本发明确保了将人体受试者给药后的人CYP3A诱导率的方便，准确的评价，在试验药物的功效，副作用的发生，药物作用的消失等方面提供准确的评价。