公开(公告)号：US20120322676A1

公开(公告)日：2012-12-20

申请号：US13495748

申请日：2012-06-13

申请人： Yongmei JI ,  Sheung-Mei ("Rita") Shih ,  Lovorka Degoricija ,  Zhaohui Zhou ,  Craig Cummings ,  Manohar Furtado ,  Pius Brzoska ,  Angela Burrell ,  Harrison Leong ,  Xingwang Fang ,  Mangkey Bounpheng ,  Rohan Shah

发明人： Yongmei JI ,  Sheung-Mei ("Rita") Shih ,  Lovorka Degoricija ,  Zhaohui Zhou ,  Craig Cummings ,  Manohar Furtado ,  Pius Brzoska ,  Angela Burrell ,  Harrison Leong ,  Xingwang Fang ,  Mangkey Bounpheng ,  Rohan Shah

IPC分类号： C12Q1/68 ,  C40B30/04 ,  G01N21/76 ,  C07H21/04 ,  G01N27/62

CPC分类号： C12Q1/689 ,  C12R1/01

摘要： Disclosed are genomic sequences for nine strains of Cronobacter spp. (C. sakazakii—696, 701, 680; C. malonaticus—507, 681; C. turicensis—564; C. muytjensii—530; C. dublinensis—582; C. genomosp1—581) and compositions, methods, and kits for detecting, identifying and distinguishing Cronobacter spp. strains from each other and from non-Cronobacter spp. strains. Some embodiments describe isolated nucleic acid compositions unique to certain Cronobacter strains as well as compositions that are specific to all Cronobacter spp. Primer and probe compositions and methods of use of primers and probes are also provided. Kits for identification of Cronobacter spp. are also described. Some embodiments relate to computer software methods for setting a control based threshold for analysis of PCR data.

摘要翻译： 公开了九个克罗杆菌属菌株的基因组序列。 （C.sakazakii-696,701,680; C. malonaticus-507,681; C. turicensis-564; C. muytjensii-530; C.dublinensis-582; C.基因组sp1-581）和组合物，方法和试剂盒 用于检测，识别和鉴别克罗杆菌属。 菌株彼此和非克罗杆菌属。 菌株。 一些实施方案描述了某些克罗杆菌菌株特有的分离的核酸组合物以及对所有克罗杆菌属特异性的组合物。 还提供引物和探针组合物以及引物和探针的使用方法。 克隆杆菌属鉴定试剂盒 也被描述。 一些实施例涉及用于设置用于PCR数据分析的基于控制的阈值的计算机软件方法。