公开(公告)号：US20130347143A1

公开(公告)日：2013-12-26

申请号：US13822131

申请日：2011-09-16

申请人： Yoshikazu Tanaka ,  Noriko Nakamura ,  Hitoshi Kobayashi ,  Hiroaki Okuhara ,  Masayoshi Kondo ,  Yosuke Koike ,  Yosuke Hoshi ,  Toshikazu Nomizu

发明人： Yoshikazu Tanaka ,  Noriko Nakamura ,  Hitoshi Kobayashi ,  Hiroaki Okuhara ,  Masayoshi Kondo ,  Yosuke Koike ,  Yosuke Hoshi ,  Toshikazu Nomizu

IPC分类号： C12N15/82

CPC分类号： C12N15/825 ,  A01H5/02 ,  A01H6/56

摘要： Provided is a method for producing lilies containing a blue pigment (delphinidin) in the petals thereof by introducing a foreign gene into lilies. The method pertaining to the present invention is a method for producing lilies containing delphinidin in the petals thereof, including the following steps: introducing, into a lily, a F3′5′H gene derived from a campanula and comprising a nucleotide sequence encoding a peptide having flavonoid 3′5′-hydroxylase (F3′5′H) activity, such as a nucleotide sequence represented by SEQ ID NO: 1 or SEQ ID NO: 11; while also introducing a F3′H gene fragment derived from a lily and comprising a nucleotide sequence encoding a peptide having flavonoid 3′-hydroxylase activity, such as a nucleotide sequence represented by SEQ ID NO: 3 or SEQ ID NO: 16; and inhibiting the expression of endogenous F3′H expression, which acts on cyanidin synthesis in lily petals, while the F3′5′H gene that has been introduced acts to cause the synthesis of delphinidin.

摘要翻译： 本发明提供了通过将外来基因引入百合中来生产其中含有蓝色色素（花翠素）的百合的方法。 本发明的方法是在其花瓣中制备含有花翠素的百合的方法，包括以下步骤：向百合中加入来自菜园的F3'5'H基因，并含有编码肽的核苷酸序列 具有类黄酮3'5'-羟化酶（F3'5'H）活性，例如由SEQ ID NO：1或SEQ ID NO：11表示的核苷酸序列; 同时引入来自百合的F3'H基因片段，并且包含编码具有类黄酮3'-羟化酶活性的肽的核苷酸序列，例如由SEQ ID NO：3或SEQ ID NO：16表示的核苷酸序列; 并抑制内源性F3'H表达，其作用于百合花瓣中的花青素合成，而已引入的F3'5'H基因则起到促进花翠素的合成作用。