公开(公告)号：US20130347143A1

公开(公告)日：2013-12-26

申请号：US13822131

申请日：2011-09-16

申请人： Yoshikazu Tanaka ,  Noriko Nakamura ,  Hitoshi Kobayashi ,  Hiroaki Okuhara ,  Masayoshi Kondo ,  Yosuke Koike ,  Yosuke Hoshi ,  Toshikazu Nomizu

发明人： Yoshikazu Tanaka ,  Noriko Nakamura ,  Hitoshi Kobayashi ,  Hiroaki Okuhara ,  Masayoshi Kondo ,  Yosuke Koike ,  Yosuke Hoshi ,  Toshikazu Nomizu

IPC分类号： C12N15/82

CPC分类号： C12N15/825 ,  A01H5/02 ,  A01H6/56

摘要： Provided is a method for producing lilies containing a blue pigment (delphinidin) in the petals thereof by introducing a foreign gene into lilies. The method pertaining to the present invention is a method for producing lilies containing delphinidin in the petals thereof, including the following steps: introducing, into a lily, a F3′5′H gene derived from a campanula and comprising a nucleotide sequence encoding a peptide having flavonoid 3′5′-hydroxylase (F3′5′H) activity, such as a nucleotide sequence represented by SEQ ID NO: 1 or SEQ ID NO: 11; while also introducing a F3′H gene fragment derived from a lily and comprising a nucleotide sequence encoding a peptide having flavonoid 3′-hydroxylase activity, such as a nucleotide sequence represented by SEQ ID NO: 3 or SEQ ID NO: 16; and inhibiting the expression of endogenous F3′H expression, which acts on cyanidin synthesis in lily petals, while the F3′5′H gene that has been introduced acts to cause the synthesis of delphinidin.

摘要翻译： 本发明提供了通过将外来基因引入百合中来生产其中含有蓝色色素（花翠素）的百合的方法。 本发明的方法是在其花瓣中制备含有花翠素的百合的方法，包括以下步骤：向百合中加入来自菜园的F3'5'H基因，并含有编码肽的核苷酸序列 具有类黄酮3'5'-羟化酶（F3'5'H）活性，例如由SEQ ID NO：1或SEQ ID NO：11表示的核苷酸序列; 同时引入来自百合的F3'H基因片段，并且包含编码具有类黄酮3'-羟化酶活性的肽的核苷酸序列，例如由SEQ ID NO：3或SEQ ID NO：16表示的核苷酸序列; 并抑制内源性F3'H表达，其作用于百合花瓣中的花青素合成，而已引入的F3'5'H基因则起到促进花翠素的合成作用。

公开(公告)号：US07514597B2

公开(公告)日：2009-04-07

申请号：US10524842

申请日：2003-08-20

申请人： Noriko Nakamura ,  Yuko Fukui ,  Eiichiro Ono ,  Yoshikazu Tanaka ,  Hiroaki Okuhara

发明人： Noriko Nakamura ,  Yuko Fukui ,  Eiichiro Ono ,  Yoshikazu Tanaka ,  Hiroaki Okuhara

IPC分类号： C12N15/29 ,  C12N15/52 ,  C12N15/82 ,  A01H5/00

CPC分类号： C12N15/8243 ,  C12N9/1048 ,  C12N15/825

摘要： The present invention provides an enzyme which catalyzes a reaction to transfer sugar to a hydroxyl group at position 2′ of chalcones and a gene thereof, and preferably an enzyme which catalyzes a reaction to transfer glucose to a hydroxyl group at position 2′ of the chalcones. Furthermore, the invention provides a plant whose flower color has been changed using the glycosyltransferase. Using probes corresponding to conservative regions of the glycosyltransferase, some tens of glycosyltransferase genes having nucleotide sequences corresponding to the conservative regions were cloned from flower petal cDNA libraries of carnation and the like. Furthermore, each of the glycosyltransferase genes was expressed in Escherichia coli, activity to transfer glucose to position 2′ of the chalcone, i.e., the glycosyltransferase activity at position 2′ of chalcone was confirmed in an extract solution of the Escherichia coli, and it was confirmed that cloned genes encoded the glycosyltransferase at position 2′.

摘要翻译： 本发明提供了一种酶，其催化将糖转移到胡烷酮的2'位的羟基及其基因的反应，优选催化将葡萄糖转移至查尔酮2'位羟基的反应的酶 。 此外，本发明提供了使用糖基转移酶改变花色的植物。 使用与糖基转移酶保守区相对应的探针，从康乃馨花花瓣cDNA文库克隆了数十个具有与保守区相对应的核苷酸序列的糖基转移酶基因。 此外，每个糖基转移酶基因在大肠杆菌中表达，将葡萄糖转移到查耳酮的位置2'的活性，即在大肠杆菌的提取液中证实了查耳酮的2'位上的糖基转移酶活性， 证实克隆的基因在2'位编码糖基转移酶。

公开(公告)号：US20060174377A1

公开(公告)日：2006-08-03

申请号：US10524842

申请日：2003-08-20

申请人： Noriko Nakamura ,  Yuko Fukui ,  Eichio Ono ,  Yoshikazu Tanaka ,  Hiroaki Okuhara

发明人： Noriko Nakamura ,  Yuko Fukui ,  Eichio Ono ,  Yoshikazu Tanaka ,  Hiroaki Okuhara

IPC分类号： A01H1/00 ,  C07H21/04 ,  C12N9/10 ,  C12N15/82 ,  C12N5/04

CPC分类号： C12N15/8243 ,  C12N9/1048 ,  C12N15/825

摘要： The present invention provides an enzyme which catalyzes a reaction to transfer sugar to a hydroxyl group at position 2′ of chalcones and a gene thereof, and preferably an enzyme which catalyzes a reaction to transfer glucose to a hydroxyl group at position 2′ of the chalcones. Furthermore, the invention provides a plant whose flower color has been changed using the glycosyltransferase. Using probes corresponding to conservative regions of the glycosyltransferase, some tens of glycosyltransferase genes having nucleotide sequences corresponding to the conservative regions were cloned from flower petal cDNA libraries of carnation and the like. Furthermore, each of the glycosyltransferase genes was expressed in Escherichia coli, activity to transfer glucose to position 2′ of the chalcone, i.e., the glycosyltransferase activity at position 2′ of chalcone was confirmed in an extract solution of the Escherichia coli, and it was confirmed that cloned genes encoded the glycosyltransferase at position 2′.

摘要翻译： 本发明提供了一种酶，其催化将糖转移到胡烷酮的2'位的羟基及其基因的反应，优选催化将葡萄糖转移至查尔酮2'位羟基的反应的酶 。 此外，本发明提供了使用糖基转移酶改变花色的植物。 使用与糖基转移酶保守区相对应的探针，从康乃馨花花瓣cDNA文库克隆了数十个具有与保守区相对应的核苷酸序列的糖基转移酶基因。 此外，每个糖基转移酶基因在大肠杆菌中表达，将葡萄糖转移到查耳酮的位置2'的活性，即在大肠杆菌的提取液中证实了查耳酮的2'位上的糖基转移酶活性， 证实克隆的基因在2'位编码糖基转移酶。

公开(公告)号：US20080318335A1

公开(公告)日：2008-12-25

申请号：US11884201

申请日：2006-02-14

申请人： Junichi Togami ,  Hiroaki Okuhara ,  Yoshikazu Tanaka

发明人： Junichi Togami ,  Hiroaki Okuhara ,  Yoshikazu Tanaka

IPC分类号： G01N21/00 ,  C12N15/63 ,  A01H5/02

CPC分类号： C12N15/8238

摘要： The present invention provides a plant transformation vector for producing a plant for detecting an environmental chemical by a change of flower color, the plant transformation vector comprising an AhR gene expressibly incorporated therein, an AhR ligand stimulus-inducible promoter, and an expression suppressive factor for suppressing the expression of a gene involved in the synthesis of a desired flower pigment, the factor being expressibly incorporated therein by the promoter.

摘要翻译： 本发明提供了一种植物转化载体，用于通过花色的变化来生产用于检测环境化学品的植物，所述植物转化载体包含可表达于其中的AhR基因，AhR配体刺激诱导型启动子和表达抑制因子 抑制参与合成所需花色素的基因的表达，该因子被启动子表达性地并入其中。

公开(公告)号：US20100287667A1

公开(公告)日：2010-11-11

申请号：US12664980

申请日：2008-06-19

申请人： Yoshikazu Tanaka ,  Yukihisa Katsumoto ,  Yuko Fukui ,  Masako Mizutani ,  Noriko Nakamura ,  Junichi Togami

发明人： Yoshikazu Tanaka ,  Yukihisa Katsumoto ,  Yuko Fukui ,  Masako Mizutani ,  Noriko Nakamura ,  Junichi Togami

IPC分类号： A01H5/00 ,  C12N5/10 ,  A01H1/00

CPC分类号： C12N15/825 ,  C12N9/0077

摘要： The invention provides a rose characterized by comprising a flavone added by a genetic modification method. The flavone is typically produced by expression of a transferred flavone synthase gene. The flavone synthase gene is, for example, a flavone synthase gene of the family Scrophulariaceae, and specifically it may be the flavone synthase gene of snapdragon of the family Scrophulariaceae, or the flavone synthase gene of torenia of the family Scrophulariaceae.

摘要翻译： 本发明提供一种玫瑰，其特征在于包含通过遗传修饰方法添加的黄酮。 黄酮通常通过转移的黄酮合成酶基因的表达产生。 黄酮合成酶基因是例如玄参科的黄酮合成酶基因，特别地，它可以是玄参科的金盏花的黄酮合成酶基因或玄参科的肾上腺素的黄酮合成酶基因。

公开(公告)号：US20100281575A1

公开(公告)日：2010-11-04

申请号：US12664998

申请日：2008-06-19

申请人： Yoshikazu Tanaka ,  Yukihisa Katsumoto ,  Yuko Fukui ,  Masako Mizutani ,  Noriko Nakamura ,  Junichi Togami

发明人： Yoshikazu Tanaka ,  Yukihisa Katsumoto ,  Yuko Fukui ,  Masako Mizutani ,  Noriko Nakamura ,  Junichi Togami

IPC分类号： A01H1/00 ,  A01H5/00

CPC分类号： C12N9/1007 ,  C12N9/0071 ,  C12N9/0073 ,  C12N15/825

摘要： The invention provides a rose characterized by comprising a flavone and malvidin added by a genetic modification method. The flavone and malvidin are typically produced by expression of a transferred flavone synthase gene, pansy flavonoid 3′,5′-hydroxylase gene and anthocyanin methyltransferase gene. The flavone synthase gene is, for example a flavone synthase gene of the family Scrophulariaceae, and specifically it may be the flavone synthase gene of snapdragon of the family Scrophulariaceae, or the flavone synthase gene of torenia of the family Scrophulariaceae. The flavonoid 3′,5′-hydroxylase gene is, for example, the pansy flavonoid 3′,5′-hydroxylase gene. The anthocyanin methyltransferase gene is, for example, the methyltransferase gene of torenia of the family Scrophulariaceae.

摘要翻译： 本发明提供了一种玫瑰，其特征在于包含通过遗传修饰方法添加的黄酮和malvidin。 通常通过转移的黄酮合成酶基因，三色堇黄酮3'，5'-羟化酶基因和花色素苷甲基转移酶基因的表达产生黄酮和malvidin。 黄酮合成酶基因是例如玄参科的黄酮合成酶基因，特别地它可以是玄参科的金盏花的黄酮合成酶基因，或玄参科的睾酮的黄酮合成酶基因。 类黄酮3'，5'-羟化酶基因是例如三色堇黄酮3'，5'-羟化酶基因。 花青素甲基转移酶基因是例如玄参科的肾上腺皮质的甲基转移酶基因。

公开(公告)号：US08350125B2

公开(公告)日：2013-01-08

申请号：US12711701

申请日：2010-02-24

申请人： Yoshikazu Tanaka ,  Eiichiro Ono ,  Noriko Nakamura ,  Masako Mizutani

发明人： Yoshikazu Tanaka ,  Eiichiro Ono ,  Noriko Nakamura ,  Masako Mizutani

IPC分类号： C12N15/29 ,  C12N15/82 ,  A01H5/00

CPC分类号： C12N15/825

摘要： There is provided a gene coding for the amino acid sequence listed as SEQ ID NO: 2 or SEQ ID NO: 70, for example. Co-expression of the 4′CGT gene and AS gene in a plant lacking natural aurone synthesis ability is carried out to successfully accumulate aurones and alter the flower color to have a yellow tint. In addition to the expression of both genes, the flavonoid pigment synthesis pathway of the host plant itself is inhibited to obtain flowers with a more defined yellow color.

摘要翻译： 提供了编码例如SEQ ID NO：2或SEQ ID NO：70所列的氨基酸序列的基因。 进行4'CGT基因和AS基因在缺乏天然鸢尾碱合成能力的植物中的共表达，以成功地积累a ones并改变花色，使其具有黄色色调。 除了两种基因的表达外，宿主植物本身的类黄酮色素合成途径被抑制以获得更明确的黄色的花。

公开(公告)号：US08206928B2

公开(公告)日：2012-06-26

申请号：US12532246

申请日：2008-03-21

申请人： Noriko Nakamura ,  Masako Mizutani ,  Yoshikazu Tanaka

发明人： Noriko Nakamura ,  Masako Mizutani ,  Yoshikazu Tanaka

IPC分类号： C12Q1/68 ,  C12P19/34

CPC分类号： C12Q1/6895 ,  C12Q2600/156

摘要： Disclosed is a method for determining whether or not a wild rose of interest is crossed with a cultivated rose. The method comprises the steps of: examining whether or not a KSN gene containing a transposon (an indicator) is contained in the rose of interest; and determining that the rose of interest is crossed with a cultivated rose when the individual has the transposon-containing KSN gene.

摘要翻译： 公开了一种用于确定野生玫瑰是否与栽培的玫瑰杂交的方法。 该方法包括以下步骤：检查含有转座子（指标）的KSN基因是否包含在感兴趣的玫瑰中; 并且当个体具有含转座子的KSN基因时，确定感兴趣的玫瑰与栽培的玫瑰杂交。

公开(公告)号：US07750209B2

公开(公告)日：2010-07-06

申请号：US10583110

申请日：2004-12-17

申请人： Yoshikazu Tanaka ,  Eiichiro Ono ,  Noriko Nakamura ,  Masako Mizutani

发明人： Yoshikazu Tanaka ,  Eiichiro Ono ,  Noriko Nakamura ,  Masako Mizutani

IPC分类号： C12N15/29 ,  C12N15/82 ,  C12N5/04 ,  A01H5/00

CPC分类号： C12N15/825

摘要： There is provided a gene coding for the amino acid sequence listed as SEQ ID NO: 2 or SEQ ID NO: 70, for example. Co-expression of the 4′CGT gene and AS gene in a plant lacking natural aurone synthesis ability is carried out to successfully accumulate aurones and alter the flower color to have a yellow tint. In addition to the expression of both genes, the flavonoid pigment synthesis pathway of the host plant itself is inhibited to obtain flowers with a more defined yellow color.

摘要翻译： 提供了编码例如SEQ ID NO：2或SEQ ID NO：70所列的氨基酸序列的基因。 进行4'CGT基因和AS基因在缺乏天然鸢尾碱合成能力的植物中的共表达，以成功地积累a ones并改变花色，使其具有黄色色调。 除了两种基因的表达外，宿主植物本身的类黄酮色素合成途径被抑制以获得更明确的黄色的花。

公开(公告)号：US20100287668A1

公开(公告)日：2010-11-11

申请号：US12665051

申请日：2008-06-19

申请人： Yoshikazu Tanaka ,  Yukihisa Katsumoto ,  Yuko Fukui ,  Masako Mizutani ,  Noriko Nakamura ,  Junichi Togami

发明人： Yoshikazu Tanaka ,  Yukihisa Katsumoto ,  Yuko Fukui ,  Masako Mizutani ,  Noriko Nakamura ,  Junichi Togami

IPC分类号： A01H5/00 ,  C12N15/82 ,  C12N5/10

CPC分类号： C12N15/825 ,  C12N9/0071 ,  C12N15/8243

摘要： The invention provides a rose characterized by comprising a flavone and delphinidin added by a genetic modification method. The flavone and delphinidin are typically produced by expression of a transferred flavone synthase gene and flavonoid 3′,5′-hydroxylase gene, respectively. The flavone synthase gene is, for example, a flavone synthase gene of the family Scrophulariaceae, and specifically it may be the flavone synthase gene of snapdragon of the family Scrophulariaceae, or the flavone synthase gene of torenia of the family Scrophulariaceae. The flavonoid 3′,5′-hydroxylase gene is, for example, the pansy (Viola×wittrockiana) flavonoid 3′,5′-hydroxylase gene.

摘要翻译： 本发明提供一种玫瑰，其特征在于包含通过遗传修饰方法添加的黄酮和花翠素。 黄酮和花翠素通常分别通过转移的黄酮合成酶基因和类黄酮3'，5'-羟化酶基因的表达产生。 黄酮合成酶基因是例如玄参科的黄酮合成酶基因，特别地，它可以是玄参科的金盏花的黄酮合成酶基因或玄参科的肾上腺素的黄酮合成酶基因。 类黄酮3'，5'-羟化酶基因为例如三色堇（Viola×wittrockiana）黄酮3'，5'-羟化酶基因。