公开(公告)号：US20130011864A1

公开(公告)日：2013-01-10

申请号：US13574511

申请日：2011-01-19

申请人： Yuan Wang ,  Guangsheng Shao ,  Rongcheng Han ,  Wen Yang ,  Yan Ma ,  Fumin Xue

发明人： Yuan Wang ,  Guangsheng Shao ,  Rongcheng Han ,  Wen Yang ,  Yan Ma ,  Fumin Xue

IPC分类号： G01N21/64 ,  C07F5/00 ,  B82Y15/00

CPC分类号： G01N33/52 ,  C07D403/14 ,  C07F5/003 ,  C09K11/06 ,  C09K2211/182 ,  G01N33/587 ,  G01N2458/40

摘要： Luminescent nanoparticles, preparation, and application thereof are disclosed. The luminescent nanoparticle consists of matrix, which is a macromolecular compound containing carboxyl group, and a rare-earth luminescent dye dispersed in the matrix. The preparation method of the luminescent nanoparticle comprises: dissolving the rare-earth complex luminescent dye and the macromolecular compound in organic solvent miscible with water, adding the solution into water, and forming the luminescent nanoparticle by coprecipitation-selfassembly process. The prepared luminescent nanoparticle has excellent long-wave excitational luminescent properties and good stability, and can be used in coupling the surface carboxyl group of a biomolecule. The biological probes based on such luminescent nanoparticles have wide application prospects on the aspects of high-sensitivity luminescent immunoassay, biological imaging and the like.

摘要翻译： 公开了发光纳米颗粒，其制备和应用。 发光纳米颗粒由分散在基质中的含有羧基的高分子化合物和稀土发光染料构成。 发光纳米颗粒的制备方法包括：将稀土配合物发光染料和高分子化合物溶解在与水混溶的有机溶剂中，将溶液加入水中，并通过共沉淀 - 自组装过程形成发光纳米颗粒。 制备的发光纳米颗粒具有优异的长波激发发光性能和良好的稳定性，可用于偶联生物分子的表面羧基。 基于这种发光纳米颗粒的生物探针在高灵敏度发光免疫测定，生物成像等方面具有广泛的应用前景。