公开(公告)号：US20090170102A1

公开(公告)日：2009-07-02

申请号：US12250480

申请日：2008-10-13

申请人： Yuk Ming Dennis LO ,  Lit Man Poon

发明人： Yuk Ming Dennis LO ,  Lit Man Poon

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6876 ,  C12Q2600/156

摘要： In a first aspect, the present invention features methods for differentiating DNA species originating from different individuals in a biological sample. These methods may be used to differentiate or detect fetal DNA in a maternal sample or to differentiate DNA of an organ donor from DNA of an organ recipient. In preferred embodiments, the DNA species are differentiated by observing epigenetic differences in the DNA species such as differences in DNA methylation. In a second aspect, the present invention features methods of detecting genetic abnormalities in a fetus by detecting fetal DNA in a biological sample obtained from a mother. In a third aspect, the present invention features methods for differentiating DNA species originating from an organ donor from those of an organ recipient. In a fourth aspect, the present invention features kits for differentiating DNA species originating from different individuals in a biological sample.

摘要翻译： 在第一方面，本发明的特征在于用于区分来自生物样品中不同个体的DNA物种的方法。 这些方法可以用于区分或检测母体样品中的胎儿DNA，或者将器官供体的DNA与器官受体的DNA区分开来。 在优选的实施方案中，DNA物种通过观察DNA物种的表观遗传学差异来区分，例如DNA甲基化的差异。 在第二方面，本发明的特征在于通过从母体获得的生物样品中检测胎儿DNA来检测胎儿遗传异常的方法。 在第三方面，本发明的特征在于用于区分源自器官供体的DNA物种与器官受体的DNA物种的方法。 在第四方面，本发明提供用于分化来自生物样品中不同个体的DNA种类的试剂盒。

公开(公告)号：US20110183330A1

公开(公告)日：2011-07-28

申请号：US12914082

申请日：2010-10-28

申请人： Yuk Ming Dennis LO ,  Rossa Wai Kwun Chiu

发明人： Yuk Ming Dennis LO ,  Rossa Wai Kwun Chiu

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6851 ,  C12Q2527/107 ,  C12Q2563/159 ,  C12Q2565/629

摘要： The present invention provides a method for analyzing nucleic acids for their lengths and relative abundance in a sample, based on digital amplification of individual template molecules. This invention has many applications, including those in noninvasive prenatal diagnosis, transplantation monitoring, and the detection and monitoring of cancers and virus-associated diseases.

摘要翻译： 本发明提供了一种基于个体模板分子的数字扩增来分析样品中核酸长度和相对丰度的方法。 本发明有许多应用，包括无创产前诊断，移植监测以及癌症和病毒相关疾病的检测和监测。

公开(公告)号：US20120003650A1

公开(公告)日：2012-01-05

申请号：US13215024

申请日：2011-08-22

申请人： Yuk Ming Dennis LO ,  Rossa Wai Kwun Chiu ,  Stephen Siu Chung Chim ,  Yu-Kwan Tong ,  Chunming Ding

发明人： Yuk Ming Dennis LO ,  Rossa Wai Kwun Chiu ,  Stephen Siu Chung Chim ,  Yu-Kwan Tong ,  Chunming Ding

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6883 ,  C12Q1/6827 ,  C12Q2600/154 ,  C12Q2600/156 ,  C12Q2521/331

摘要： The present invention relates to new methods for diagnosing a pregnancy-associated disorder by analyzing fetal DNA present in the mother's blood. More specifically, this invention relies on the discovery that the maspin gene is differentially methylated in fetal DNA and in maternal DNA and provides these new diagnostic methods, which distinguish fetal DNA from maternal DNA and detect prenatal disorders based on abnormalities in fetal DNA level and methylation status.

摘要翻译： 本发明涉及通过分析存在于母亲血液中的胎儿DNA来诊断妊娠相关疾病的新方法。 更具体地说，本发明依赖于maspin基因在胎儿DNA和母体DNA中差异甲基化的发现，并提供了这些新的诊断方法，其将胎儿DNA与母体DNA区分开，并根据胎儿DNA水平和甲基化异常检测产前疾病 状态。