公开(公告)号：US20250002882A1

公开(公告)日：2025-01-02

申请号：US18658938

申请日：2024-05-08

Applicant: ZHEJIANG LAB

Inventor： JIN TANG ,  JUNCHAO SHI ,  YUANYUAN FENG ,  PEIXIANG MA ,  XINGXU HUANG

IPC: C12N9/22 ,  C12N15/11 ,  C12N15/63

Abstract: The present invention discloses a Cpf 1 protein and its use in gene editing, which relates to the field of biotechnology. The present invention provides a novel Cas12a nuclease named as the Cas12a-68 protease, which has a wider PAM recognition range than traditional nucleases and is capable of recognizing targets of various genes. When editing nucleic acid fragments, using the new nuclease of the present invention is capable of overcoming the shortcomings of the traditional Cas12a and increasing the PAM recognition range. These advantages make the present invention more suitable for editing a wider range of nucleic acid fragments and lay a foundation for gene editing applications.