公开(公告)号：US12031160B2

公开(公告)日：2024-07-09

申请号：US17419048

申请日：2019-06-28

申请人： ZHEJIANG UNIVERSITY OF TECHNOLOGY

发明人： Yaping Xue ,  Feng Cheng ,  Qinghua Li ,  Yuguo Zheng ,  Jianmiao Xu

IPC分类号： C12P13/04 ,  C12N9/06

CPC分类号： C12N9/0016 ,  C12P13/04

摘要： The present invention provides a glufosinate-ammonium dehydrogenase mutant and application in synthesis of L-glufosinate-ammonium thereof, the method uses 2-carbonyl-4-[(hydroxy)(methyl)phosphinoyl]-butyric acid or its salts as a substrate and the glufosinate-ammonium dehydrogenase or cells containing the glufosinate-ammonium dehydrogenase as a biocatalyst to carry out reductive amination, thereby obtaining L-glufosinate-ammonium. The method has features of high conversion rate of raw materials, high yield, easy separation and purification of the product, and high chiral purity; compared with other catalytic processes, the method in the present invention has features of relatively simple process and a conversion rate of raw materials up to 100%.