公开(公告)号：US12194145B2

公开(公告)日：2025-01-14

申请号：US17227408

申请日：2021-04-12

Applicant: ZHENGZHOU UNIVERSITY

Inventor： Zhimin Wang ,  Chengshen Zhu ,  Wentao Liu ,  Xiangyang Zhang ,  Suqin He ,  Yaohe Wang ,  Hao Liu ,  Miaoming Huang ,  Chenglin Zhang ,  Bin Qin ,  Lei Huang

IPC: A61K9/127 ,  A61K9/51 ,  A61K31/713 ,  B82Y5/00 ,  C08B37/08

Abstract: The invention relates to the technical field of gene treatment and nano targeted delivery, in particular to a siRNA delivery system compound as well as a preparation method and application thereof. The preparation method comprises the following steps of reacting Boc-protected histidine with cholesterol to generate Boc-protected histidine cholesteryl ester, and removing BOC-released protected amino in trifluoroacetic acid to generate histidine cholesteryl ester; then, enabling amino to react with carboxyl of carboxymethyl chitosan to modify long-chain carboxymethyl chitosan to generate carboxymethyl chitosan modified by histidine cholesteryl ester, and performing targeted labeling on carboxymethyl chitosan modified by histidine cholesteryl ester to generate a carboxymethyl chitosan high-molecular compound modified by histidine cholesteryl ester and a targeted marker together, then forming a stable spherical delivery vector through self-assembly above critical micelle concentration, and mixing the stable spherical delivery vector with siRNA (small interfering ribonucleic acid) to obtain the siRNA delivery system compound.