公开(公告)号：US10131689B2

公开(公告)日：2018-11-20

申请号：US15033116

申请日：2014-10-27

申请人： Zhejiang Medicine Co., Ltd. Xinchang Pharmaceutical Factory

发明人： Enmin Li ,  Yiyun Zhuang ,  Jue Wang ,  Xinqiang Sun ,  Xuejun Lao ,  Biwang Jiang

IPC分类号： C07K1/36 ,  C07K9/00 ,  A61K38/14 ,  C07K1/20 ,  C07K1/34

摘要： Provided is a separation and purification method for vancomycin hydrochloride of high purity. The method comprises the following steps: (1) obtaining a vancomycin hydrochloride solution from a crude vancomycin product by ion exchange chromatography and obtaining a concentrate by nanofiltration desalination and concentration; (2) adjusting the concentrate with a hydrochloric acid solution and then performing a column chromatography using a reverse chromatography column for the adjusted concentrate; (3) collecting the chromatographic solution of vancomycin to obtain a mixed chromatographic solution; (4) adjusting the mixed chromatographic solution, and separating the solution and the salts by nanofiltration desalination and concentration to obtain a concentrate; and (5) obtaining a vancomycin dry powder with a chromatographic purity of up to 99% and a pure white appearance by dehydrating and drying the concentrate of step (4), or by solvent crystallization or salting-out crystallization.