公开(公告)号：US20170096652A1

公开(公告)日：2017-04-06

申请号：US15378035

申请日：2016-12-13

Applicant: Zhejiang University

Inventor： Yuejin Hua ,  Yunguang Wang ,  Liangyan Wang

IPC: C12N9/52

CPC classification number: C12N9/52 ,  C12Y304/00 ,  G10H1/0066 ,  G10H1/0551 ,  G10H1/18 ,  G10H2220/096 ,  G10H2220/121 ,  G10H2220/241 ,  G10H2220/275 ,  G10H2220/461

Abstract: The present invention relates to a polypeptide having protease activity comprising a zinc finger protease domain, a helix-turn-helix domain and a GAF domain. The core protein sequence of the protease is shown as SEQ ID NO: 1. The invention also relates to optimized reaction conditions for the protease and methods of increasing the protease activity.

公开(公告)号：US10316310B2

公开(公告)日：2019-06-11

申请号：US15378035

申请日：2016-12-13

Applicant: Zhejiang University

Inventor： Yuejin Hua ,  Yunguang Wang ,  Liangyan Wang

IPC: C12N9/52 ,  G10H1/055 ,  G10H1/18 ,  G10H1/00

Abstract: The present invention relates to a polypeptide having protease activity comprising a zinc finger protease domain, a helix-turn-helix domain and a GAF domain. The core protein sequence of the protease is shown as SEQ ID NO: 1. The invention also relates to optimized reaction conditions for the protease and methods of increasing the protease activity.