公开(公告)号：US20160206781A1

公开(公告)日：2016-07-21

申请号：US14983283

申请日：2015-12-29

申请人： Zurab KAKABADZE ,  David CHAKHUNASHVILI ,  Konstantine CHAKHUNASHVILI ,  Nicholas KIPSHIDZE ,  Ann KAKABADZE

发明人： Zurab KAKABADZE ,  David CHAKHUNASHVILI ,  Konstantine CHAKHUNASHVILI ,  Nicholas KIPSHIDZE ,  Ann KAKABADZE

IPC分类号： A61L27/36 ,  A61L27/50 ,  B65B63/08 ,  A61L27/38

CPC分类号： A61L27/3608 ,  A61L27/3687 ,  A61L27/3691 ,  A61L27/3821 ,  A61L27/3834 ,  A61L2430/02

摘要： The invention disclosed herein relates to methods and compositions useful for the production of composites for bone implantation. The invention includes methods for production of lyophilized bone fragments seeded with lyophilized stem cells, mesenchymal stem cells, bone marrow stem cells, periosteal cells or osteocytes that are derived either from the recipient of the bone implant or from allogeneic sources. The methods of the invention comprise production of bone implant material including the steps of cutting solid bone into fragments, decellularizing the bone fragments, seeding stem cells, mesenchymal stem cells, bone marrow stem cells, periosteal cells or osteocytes onto the decellularized bone fragments and lyophilizing the complex of bone fragments and cells. The stabilized bone matrix and complex of bone and cells increases the ease of transport, storage and reconstitution of bone and cells for bone implantation.

摘要翻译： 本文公开的本发明涉及可用于生产用于骨植入的复合材料的方法和组合物。 本发明包括用冻干的干细胞，间充质干细胞，骨髓干细胞，骨膜细胞或骨细胞接种的冻干骨碎片的方法，所述干细胞干细胞，骨髓细胞或骨细胞从骨植入物的接受者或同种异体来源衍生。 本发明的方法包括骨植入材料的生产，包括将固体骨切成碎片，将骨碎片，接种干细胞，间充质干细胞，骨髓干细胞，骨膜细胞或骨细胞脱细胞到脱细胞骨碎片上的步骤和冻干 骨碎片和细胞的复合体。 稳定的骨基质和骨和细胞的复合物增加骨和移植骨骼和细胞的运输，储存和重建的容易性。