Method for measuring the activity of deacetylase and method of screening for inhibitors and accelerators of the enzyme
    3.
    发明申请
    Method for measuring the activity of deacetylase and method of screening for inhibitors and accelerators of the enzyme 有权
    测定脱乙酰酶活性的方法和筛选酶抑制剂和促进剂的方法

    公开(公告)号:US20030082668A1

    公开(公告)日:2003-05-01

    申请号:US10157382

    申请日:2002-05-29

    申请人: CYCLEX CO., LTD

    IPC分类号: C12Q001/37 C07K001/00

    摘要: The acetylation level of a peptide is determined utilizing the fact that the changes in the acetylation level are reflected in the sensitivity of the substrate peptide to a peptidase. This method can be used for measuring activities of deacetylase and acetylase, and also enables screening for substances that influence the activity of these enzymes. The deacetylase activity can be measured by a simple procedure according to the present invention.

    摘要翻译: 使用乙酰化水平的变化反映在底物肽对肽酶的敏感性的事实来确定肽的乙酰化水平。 该方法可用于测定脱乙酰酶和乙酰酶的活性,也可用于筛选影响这些酶活性的物质。 脱乙酰酶活性可以通过根据本发明的简单程序来测量。