公开(公告)号：US20110022326A1

公开(公告)日：2011-01-27

申请号：US12787712

申请日：2010-05-26

申请人： Yoshiya ODA ,  Yasushi Ishihama ,  Tsuyoshi Tabata

发明人： Yoshiya ODA ,  Yasushi Ishihama ,  Tsuyoshi Tabata

IPC分类号： G06F19/00 ,  C12N5/07 ,  C12Q1/02 ,  C07C229/08

CPC分类号： G01N33/6848 ,  Y10T436/10 ,  Y10T436/104165 ,  Y10T436/104998 ,  Y10T436/105831 ,  Y10T436/24

摘要： Problem: An object of the present invention is to quantitate with good accuracy, furthermore, quantitate absolutely, one or a plurality of biological molecules in a sample such as a tissue, a biological fluid, a cell, a cell organ or protein complex.Solution: By adding a metabolically isotope labeled biological molecule as an internal standard substance and measuring with a mass spectrometer, quantitating with good accuracy one or a plurality of target molecules in a sample has become possible. In addition, by performing waveform separation processing during mass analysis, a highly accurate quantitative analysis method of mass analysis is provided.

摘要翻译： 问题：本发明的目的是准确定量，此外，在样品如组织，生物流体，细胞，细胞器官或蛋白质复合物中绝对地定量分析一种或多种生物分子。 解决方案：通过添加代谢同位素标记的生物分子作为内标物质并用质谱仪进行测量，以高精度定量，样品中的一个或多个目标分子成为可能。 此外，通过在质量分析中进行波形分离处理，提供了高精度的质量分析的定量分析方法。