-
1.
公开(公告)号:US11708613B2
公开(公告)日:2023-07-25
申请号:US16609994
申请日:2018-04-27
发明人: Amanda E. Calvert
IPC分类号: C12Q1/6888 , C12Q1/70 , C12Q1/6844
CPC分类号: C12Q1/6888 , C12Q1/6844 , C12Q1/701 , C12Q2525/301 , C12Q2531/119 , C12Q2600/16
摘要: A highly sensitive and specific reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay to detect ZIKV nucleic acid in biological samples is described. The disclosed assay is capable of detecting as few as one RNA copy per μl and can be performed in a clinical or field setting with minimal equipment and technological expertise. Oligonucleotide primers and kits for detecting ZIKV nucleic acid are also described.