公开(公告)号：US09491943B2

公开(公告)日：2016-11-15

申请号：US14356636

申请日：2011-11-21

申请人： Yuquan Xu ,  Huifeng Shen ,  Ya-Wen He ,  Wanping Zhou

发明人： Yuquan Xu ,  Huifeng Shen ,  Ya-Wen He ,  Wanping Zhou

IPC分类号： C12N1/20 ,  A01N43/58 ,  A01N43/60 ,  C12P17/12 ,  C12N15/63 ,  C12N9/00

CPC分类号： A01N43/60 ,  C12N1/20 ,  C12N9/93 ,  C12N15/63 ,  C12P17/12 ,  C12Y603/05004 ,  Y02P20/52

摘要： The present invention relates to a bioengineering strain for production of novel microorganism-originated fungicides and uses thereof. The bioengineering strain for production of microorganism-originated fungicides of the present invention is obtained by transforming a phzH gene recombination expression plasmid into a strain producing phenazine-1-carboxylic acid, wherein the bioengineering strain produces phenazine-1-carboxamide. The present invention utilizes an existing strain producing phenazine-1-carboxylic acid to carry the phzH gene recombination expression plasmid, thereby achieving efficient expression of the phzH gene and transforming phenazine-1-carboxylic acid into phenazine-1-carboxamide. The present invention further discloses uses of the bioengineering strain, including a microorganism-originated fungicide produced by the bioengineering strain through fermentation, and preparation and application of the microorganism-originated fungicide. The antifungal activity of phenazine-1-carboxamide is not influenced by the acidity under which it is used, so that it has a stabilized antifungal activity and can be more effective in prophylaxis and treatment of crop diseases.

摘要翻译： 本发明涉及用于生产新型微生物来源的杀真菌剂的生物工程菌株及其应用。 通过将phzH基因重组表达质粒转化到产生吩嗪-1-羧酸的菌株中，其中生物工程菌株产生吩嗪-1-甲酰胺，获得了用于生产本发明的微生物来源的杀真菌剂的生物工程菌株。 本发明利用产生吩嗪-1-羧酸的现有菌株携带phzH基因重组表达质粒，从而实现phzH基因的有效表达和将吩嗪-1-羧酸转化为吩嗪-1-甲酰胺。 本发明还公开了生物工程菌株的应用，其包括由生物工程菌株通过发酵生产的微生物来源的杀真菌剂，以及微生物来源的杀真菌剂的制备和应用。 吩嗪-1-甲酰胺的抗真菌活性不受其使用酸度的影响，使其具有稳定的抗真菌活性，可以更有效地预防和治疗作物病害。

公开(公告)号：US20140309232A1

公开(公告)日：2014-10-16

申请号：US14356636

申请日：2011-11-21

申请人： Yuquan Xu ,  Huifeng Shen ,  Ya-Wen He ,  Wanping Zhou

发明人： Yuquan Xu ,  Huifeng Shen ,  Ya-Wen He ,  Wanping Zhou

IPC分类号： A01N43/60 ,  C12N15/63

CPC分类号： A01N43/60 ,  C12N1/20 ,  C12N9/93 ,  C12N15/63 ,  C12P17/12 ,  C12Y603/05004 ,  Y02P20/52

摘要： The present invention relates to a bioengineering strain for production of novel microorganism-originated fungicides and uses thereof. The bioengineering strain for production of microorganism-originated fungicides of the present invention is obtained by transforming a phzH gene recombination expression plasmid into a strain producing phenazine-1-carboxylic acid, wherein the bioengineering strain produces phenazine-1-carboxamide. The present invention utilizes an existing strain producing phenazine-1-carboxylic acid to carry the phzH gene recombination expression plasmid, thereby achieving efficient expression of the phzH gene and transforming phenazine-1-carboxylic acid into phenazine-1-carboxamide. The present invention further discloses uses of the bioengineering strain, including a microorganism-originated fungicide produced by the bioengineering strain through fermentation, and preparation and application of the microorganism-originated fungicide. The antifungal activity of phenazine-1-carboxamide is not influenced by the acidity under which it is used, so that it has a stabilized antifungal activity and can be more effective in prophylaxis and treatment of crop diseases.

摘要翻译： 本发明涉及用于生产新型微生物来源的杀真菌剂的生物工程菌株及其应用。 通过将phzH基因重组表达质粒转化到产生吩嗪-1-羧酸的菌株中，其中生物工程菌株产生吩嗪-1-甲酰胺，获得了用于生产本发明的微生物来源的杀真菌剂的生物工程菌株。 本发明利用产生吩嗪-1-羧酸的现有菌株携带phzH基因重组表达质粒，从而实现phzH基因的有效表达和将吩嗪-1-羧酸转化为吩嗪-1-甲酰胺。 本发明还公开了生物工程菌株的应用，其包括由生物工程菌株通过发酵生产的微生物来源的杀真菌剂，以及微生物来源的杀真菌剂的制备和应用。 吩嗪-1-甲酰胺的抗真菌活性不受其使用酸度的影响，使其具有稳定的抗真菌活性，可以更有效地预防和治疗作物病害。