公开(公告)号：US08029803B2

公开(公告)日：2011-10-04

申请号：US10365620

申请日：2003-02-13

申请人： Rajan George ,  Lorne Tyrrell ,  Antoine Noujaim

发明人： Rajan George ,  Lorne Tyrrell ,  Antoine Noujaim

IPC分类号： A61K399/29 ,  C12P21/00

CPC分类号： C07K14/005 ,  A61K39/00 ,  A61K39/385 ,  A61K2039/57 ,  A61K2039/6056 ,  C07K2319/00 ,  C07K2319/01 ,  C07K2319/21 ,  C07K2319/30 ,  C12N2710/14143 ,  C12N2730/10122 ,  C12N2770/24222

摘要： Disclosed herein are the nucleotide sequences, deduced amino acid sequences as well as methods and compositions necessary to elicit immune responses against chronic Hepatitis B infections in animals and humans. Immune response is enhanced by fusing relevant viral antigens with xenotypic immunoglobulin heavy chain region through a peptide linker and producing the fusion proteins in Baculovirus expression system to incorporate high mannose glycosylation. By virtue of the antibody component, the fusion proteins bind to Fc receptors on the surface of antigen presenting cells, are taken up, processed and derived peptides are presented on MHC Class I, which elicit a CTL (Th1) response. In a similar fashion, due to cross priming and presentation on MHC Class II, will elicit a humoral (Th2) response. In addition, disclosed are the methods of cloning, expression and production of the fusion proteins.

摘要翻译： 本文公开了核苷酸序列，推定的氨基酸序列以及在动物和人中引发针对慢性乙型肝炎感染的免疫应答所必需的方法和组合物。 通过肽接头将相关病毒抗原与异型免疫球蛋白重链区融合并在杆状病毒表达系统中产生融合蛋白以掺入高甘露糖糖基化来增强免疫应答。 由于抗体组分，融合蛋白与抗原呈递细胞表面上的Fc受体结合，被吸收，加工，并且衍生的肽呈现在引起CTL（Th1）应答的MHC I类上。 以类似的方式，由于交叉引物和介绍MHC II类，将引发体液（Th2）反应。 此外，公开了融合蛋白的克隆，表达和产生的方法。

公开(公告)号：US08025873B2

公开(公告)日：2011-09-27

申请号：US10912969

申请日：2004-08-05

申请人： Rajan George ,  Lorne Tyrrell ,  Antoine Noujaim ,  Dakun Wang ,  Allan Ma

发明人： Rajan George ,  Lorne Tyrrell ,  Antoine Noujaim ,  Dakun Wang ,  Allan Ma

IPC分类号： A61K399/29 ,  C12P21/00

CPC分类号： C07K16/46 ,  A61K39/00 ,  A61K39/385 ,  A61K2039/57 ,  A61K2039/6056 ,  C07K14/005 ,  C07K2319/00 ,  C07K2319/01 ,  C07K2319/21 ,  C07K2319/30 ,  C12N2710/14143 ,  C12N2730/10122 ,  C12N2770/24222 ,  C12P21/005

摘要： Disclosed herein are compositions and methods for eliciting immune responses against antigens. In particular embodiments, the compounds and methods elicit immune responses against antigens that are otherwise recognized by the host as “self” antigens. The immune response is enhanced by presenting the host immune system with a chimeric antigen comprising an immune response domain and a target binding domain, wherein the target binding domain comprises a xenotypic antibody fragment. By virtue of the target binding domain, antigen presenting cells take up, process, and present the chimeric antigen, eliciting both a humoral and cellular immune response.

摘要翻译： 本文公开了用于引发针对抗原的免疫应答的组合物和方法。 在具体实施方案中，化合物和方法引发抗原的免疫应答，否则被宿主所认为的是“自身”抗原。 通过用宿主免疫系统提供包含免疫应答结构域和靶结合结构域的嵌合抗原来增强免疫应答，其中靶结合结构域包含异型抗体片段。 由于靶结合域，抗原呈递细胞吸收，处理和存在嵌合抗原，引起体液和细胞免疫应答。