公开(公告)号：US07186815B2

公开(公告)日：2007-03-06

申请号：US10431577

申请日：2003-05-08

申请人： Peter Ravn ,  Soeren Michael Madsen ,  Astrid Vrang ,  Hans Israelsen ,  Mads Groenvold Johnsen ,  Lars Bredmose ,  José Arnau

发明人： Peter Ravn ,  Soeren Michael Madsen ,  Astrid Vrang ,  Hans Israelsen ,  Mads Groenvold Johnsen ,  Lars Bredmose ,  José Arnau

IPC分类号： C07H21/02 ,  C07H21/04 ,  C07N7/00 ,  C07N15/00 ,  C07N15/09

CPC分类号： C12N15/1034 ,  C07K2319/02 ,  C07K2319/036 ,  C07K2319/61 ,  C12N15/625 ,  C12N15/746

摘要： A method of identifying nucleotide sequences coding for signal peptides in lactic acid bacteria, using a DNA molecule comprising a transposon including a promoterless reporter gene from which DNA molecule a region between the LR and the reporter gene is deleted and the DNA molecule comprises a DNA sequence coding for a secretion reporter molecule. By deleting the region between the LR and the reporter gene, stop codons in-frame with the secretion reporter molecule is removed which upon transposition permits translational fusions from upstream the LR.

摘要翻译： 使用包含转座子的DNA分子的DNA分子的方法，所述DNA分子包含转座子，所述转座子包含DNA分子，LR分子和报告基因之间的区域由DNA启动子报告基因组成，DNA分子包含DNA序列 编码分泌报道分子。 通过删除LR和报告基因之间的区域，消除了与分泌报告分子的框内的密码子，其在转座后允许从LR的上游进行翻译融合。

公开(公告)号：US06893858B2

公开(公告)日：2005-05-17

申请号：US09866020

申请日：2001-05-24

申请人： Steven I. Dworetzky ,  Chandra S. Ramanathan ,  Joanne T. Trojnacki ,  Valentin K. Gribkoff

发明人： Steven I. Dworetzky ,  Chandra S. Ramanathan ,  Joanne T. Trojnacki ,  Valentin K. Gribkoff

IPC分类号： G01N33/50 ,  A61K31/7088 ,  A61K35/76 ,  A61K38/00 ,  A61K45/00 ,  A61K48/00 ,  A61P11/06 ,  A61P17/02 ,  A61P21/00 ,  A61P25/00 ,  A61P25/04 ,  A61P25/06 ,  A61P25/08 ,  A61P25/16 ,  A61P25/22 ,  A61P25/24 ,  A61P25/28 ,  A61P25/30 ,  A61P35/00 ,  A61P43/00 ,  C07K14/47 ,  C07K14/705 ,  C07K16/18 ,  C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  C12N5/10 ,  C12N15/09 ,  C12N15/12 ,  C12P21/08 ,  C12Q1/02 ,  G01N33/15 ,  G01N37/00 ,  C07H21/04 ,  C07K14/00 ,  C12N15/63 ,  C07N15/00

CPC分类号： C07K14/705 ,  A61K38/00 ,  C07K2319/00

摘要： An isolated polynucleotide encoding a novel potassium channel polypeptide, KCNQ5, that is expressed primarily in brain and skeletal muscle is described. The new polypeptide has been cloned and isolated from a human brain cDNA library and is a member the KCNQ family of potassium channels. The provided human KCNQ5 nucleic acid sequence and encoded polypeptide can be employed for diagnostic, screening and therapeutic uses. Moreover, the hKCNQ5 polypeptide can be used to assay for KCNQ5 potassium channel modulators, which can be utilized in the treatment of neurological, neurophysiological, neuropsychological and neuroaffective diseases, conditions and disorders, including, but not limited to, acute and chronic pain, migraine, acute stroke, dementia, vascular dementia, trauma, epilepsy, amyelotrophic lateral sclerosis (ALS), multiple sclerosis (MS), Parkinson's Disease, learning and cognitive disorders, and neurophysiological disorders including anxiety disorders, depression, bipolar disorders, sleep disorders, addiction, and eating disorders.

摘要翻译： 描述了编码主要在脑和骨骼肌中表达的新型钾通道多肽KCNQ5的分离的多核苷酸。 新的多肽已经从人脑cDNA文库克隆和分离，并且是钾通道KCNQ家族的成员。 提供的人类KCNQ5核酸序列和编码的多肽可用于诊断，筛选和治疗用途。 此外，hKCNQ5多肽可用于测定KCNQ5钾通道调节剂，其可用于治疗神经，神经生理学，神经心理学和神经感染性疾病，病症和障碍，包括但不限于急性和慢性疼痛，偏头痛 ，急性中风，痴呆，血管性痴呆，创伤，癫痫，青光眼性侧索硬化（ALS），多发性硬化症（MS），帕金森病，学习和认知障碍以及神经生理学障碍，包括焦虑症，抑郁症，双相情感障碍，睡眠障碍，成瘾 和饮食失调。

公开(公告)号：US06875569B2

公开(公告)日：2005-04-05

申请号：US09952559

申请日：2001-09-13

申请人： Fred H. Gage ,  Steven T. Suhr

发明人： Fred H. Gage ,  Steven T. Suhr

IPC分类号： A61K38/00 ,  A61K48/00 ,  C07K14/72 ,  C12Q1/68 ,  C07K14/00 ,  C07N15/00 ,  C07N15/63 ,  C07N21/04

CPC分类号： C07K14/721 ,  A01K2217/05 ,  A61K38/00 ,  A61K48/00 ,  C07K2319/00 ,  C12N2799/027

摘要： In accordance with the present invention, it has been discovered that nuclear receptor proteins isolated from the silk moth bombyx mori (bR) are useful for the regulation of transgene expression. bR is generally thought to be a strong transcriptional regulator within cells of the silk moth. In accordance with the present invention, it has been discovered that bR is also functional in mammalian cells. It has further been discovered that the addition of activation domains to the bR open-reading frame enhances the activity of the ligand modulated regulator to afford high-level transcriptional induction. Further modifications to the bR ligand binding domain result in receptors with unique transactivational characteristics.

摘要翻译： 根据本发明，已经发现从蚕蚕（bR）分离的核受体蛋白质可用于调节转基因表达。 bR通常被认为是丝蛾细胞内的强转录调控因子。 根据本发明，已经发现bR在哺乳动物细胞中也起作用。 还已经发现，将激活结构域添加到bR开放阅读框架中增强配体调节调节物的活性以提供高水平的转录诱导。 对bR配体结合域的进一步修饰导致具有独特的反式活化特征的受体。