公开(公告)号：US06514295B1

公开(公告)日：2003-02-04

申请号：US09290248

申请日：1999-04-13

申请人： Don J. Chandler ,  Beth A. Lambert ,  Janet J. Reber ,  Stacie L. Phipps

发明人： Don J. Chandler ,  Beth A. Lambert ,  Janet J. Reber ,  Stacie L. Phipps

IPC分类号： D06P190

CPC分类号： G01N33/533 ,  C12Q1/6816 ,  D06P1/0012 ,  D06P1/922 ,  D06P1/924 ,  D06P1/926 ,  D06P1/928 ,  G01N33/54313 ,  Y10T428/2982 ,  Y10T428/2989 ,  Y10T428/2991 ,  Y10T428/2998

摘要： An improved method of making a series of bead or microsphere or particle populations characterized by subtle variation in a proportion or ratio of at least two fluorescent dyes distributed within a single bead of each population is provided. These beads, when excited by a single excitation light source are capable of giving off several fluorescent signals simultaneously. A set containing as many as 64 distinct populations of multicolored, fluorescent beads is provided and when combined with analytical reagents bound to the surface of such beads is extremely useful for multiplexed analysis of a plurality of analytes in a single sample. Thus, methods of staining polymeric particles, the particles themselves, and methods of using such particles are claimed.

公开(公告)号：US06599331B2

公开(公告)日：2003-07-29

申请号：US10185869

申请日：2002-07-01

申请人： Don J. Chandler ,  Beth A. Lambert ,  Janet J. Reber ,  Stacie L. Phipps

发明人： Don J. Chandler ,  Beth A. Lambert ,  Janet J. Reber ,  Stacie L. Phipps

IPC分类号： D06P190

CPC分类号： G01N33/533 ,  C12Q1/6816 ,  D06P1/0012 ,  D06P1/922 ,  D06P1/924 ,  D06P1/926 ,  D06P1/928 ,  G01N33/54313 ,  Y10T428/2982 ,  Y10T428/2989 ,  Y10T428/2991 ,  Y10T428/2998

摘要： An improved method of making a series of bead or microsphere or particle populations characterized by subtle variation in a proportion or ratio of at least two fluorescent dyes distributed within a single bead of each population is provided. These beads, when excited by a single excitation light source are capable of giving off several fluorescent signals simultaneously. A set containing as many as 64 distinct populations of multicolored, fluorescent beads is provided and when combined with analytical reagents bound to the surface of such beads is extremely useful for multiplexed analysis of a plurality of analytes in a single sample. Thus, methods of staining polymeric particles, the particles themselves, and methods of using such particles are claimed.

摘要翻译： 提供了一种制备一系列珠或微球或颗粒群的改进方法，其特征在于分布在每个群体的单个珠粒内的至少两种荧光染料的比例或比例的微小变化。 这些珠当被单个激发光源激发时能够同时发出几个荧光信号。 提供了含有多达64个不同种群的多色荧光珠的组，并且当与结合到这种珠的表面的分析试剂组合时，对于单个样品中的多种分析物的多重分析非常有用。 因此，要求保护染色聚合物颗粒，颗粒本身和使用这些颗粒的方法。