公开(公告)号：US06905766B2

公开(公告)日：2005-06-14

申请号：US10355073

申请日：2003-01-31

Applicant: Don J. Chandler

Inventor： Don J. Chandler

IPC: B01J13/04 ,  B01J13/18 ,  G01N15/10 ,  G01N33/58 ,  B32B15/02 ,  B01J13/02

CPC classification number: G01N33/587 ,  B01J13/046 ,  B01J13/18 ,  C12Q2563/107 ,  C12Q2563/149 ,  G01N2015/1018 ,  G01N2333/78 ,  G01N2400/26 ,  G01N2400/28 ,  Y10T428/2984 ,  Y10T428/2985 ,  Y10T428/2991

Abstract: The present invention provides novel encapsulation compositions and methods. In particular, the invention relates to fluorescent capsule compositions, which consists of a layer of a polymer shell enclosing one or more fluorescent materials such as fluorescent microspheres and which are capable of emitting at least two distinct fluorescent signals. Also provided are methods for their preparation. The compositions and methods of this invention are useful in a variety of applications, including preparation of multiplexed arrays for industrial, chemical, immunological, and genetic manipulation and analysis especially as related but not limited to flow cytometry.

Abstract translation: 本发明提供了新颖的包封组合物和方法。 特别地，本发明涉及荧光胶囊组合物，其由包围一种或多种荧光材料如荧光微球的聚合物壳层组成，并且能够发射至少两种不同的荧光信号。 还提供了它们的制备方法。 本发明的组合物和方法可用于各种应用，包括制备用于工业，化学，免疫和遗传操作和分析的复合阵列，特别是相关但不限于流式细胞术。

公开(公告)号：US06528165B2

公开(公告)日：2003-03-04

申请号：US10103807

申请日：2002-03-25

Applicant: Don J. Chandler

Inventor： Don J. Chandler

IPC: B32B1502

CPC classification number: G01N33/587 ,  B01J13/046 ,  B01J13/18 ,  C12Q2563/107 ,  C12Q2563/149 ,  G01N2015/1018 ,  G01N2333/78 ,  G01N2400/26 ,  G01N2400/28 ,  Y10T428/2984 ,  Y10T428/2985 ,  Y10T428/2991

Abstract: The present invention provides novel encapsulation compositions and methods. In particular, the invention relates to fluorescent capsule compositions, which consists of a layer of a polymer shell enclosing one or more fluorescent materials such as fluorescent microspheres and which are capable of emitting at least two distinct fluorescent signals. Also provided are methods for their preparation. The compositions and methods of this invention are useful in a variety of applications, including preparation of multiplexed arrays for industrial, chemical, immunological, and genetic manipulation and analysis especially as related but not limited to flow cytometry.

公开(公告)号：US08283037B2

公开(公告)日：2012-10-09

申请号：US12731290

申请日：2010-03-25

Applicant: Don J. Chandler ,  Jason Bedre

Inventor： Don J. Chandler ,  Jason Bedre

IPC: B32B5/66

CPC classification number: G01N33/54326 ,  B82Y25/00 ,  C09K11/025 ,  C09K11/06 ,  G01N21/64 ,  G01N21/6428 ,  G01N21/6486 ,  H01F1/0018 ,  H01F1/0036 ,  H01F1/0315 ,  H01F1/063 ,  H01F1/111 ,  H01F1/36 ,  Y10T428/2991 ,  Y10T428/2993 ,  Y10T428/2995 ,  Y10T428/2996 ,  Y10T428/2998 ,  Y10T428/32

Abstract: Microspheres, populations of microspheres, and methods for forming microspheres are provided. One microsphere configured to exhibit fluorescent and magnetic properties includes a core microsphere and a magnetic material coupled to a surface of the core microsphere. About 50% or less of the surface of the core microsphere is covered by the magnetic material. The microsphere also includes a polymer layer surrounding the magnetic material and the core microsphere. One population of microspheres configured to exhibit fluorescent and magnetic properties includes two or more subsets of microspheres. The two or more subsets of microspheres are configured to exhibit different fluorescent and/or magnetic properties. Individual microspheres in the two or more subsets are configured as described above.

Abstract translation: 提供了微球，微球体和形成微球的方法。 配置为显示荧光和磁性的一个微球包括芯微球和耦合到芯微球表面的磁性材料。 核心微球表面的约50％以下被磁性材料覆盖。 微球还包括围绕磁性材料和芯微球的聚合物层。 配置成显示荧光和磁性的一组微球体包括两个或更多个微球子集。 两个或多个微球子集被配置为表现出不同的荧光和/或磁性。 两个或多个子集中的单个微球体如上所述配置。

公开(公告)号：US07260485B2

公开(公告)日：2007-08-21

申请号：US10885420

申请日：2004-07-06

Applicant: Don J. Chandler

Inventor： Don J. Chandler

IPC: G06F19/00

CPC classification number: G01N21/645 ,  G01N2021/6421

Abstract: Various computer-implemented methods and systems are provided. One computer-implemented method includes determining a ratio between output signals generated by detecting spectra for a single event in two or more detection windows. The spectra are characteristic of different materials. At least a portion of the spectra overlap in at least one of the two or more detection windows. The method also includes determining which of the different materials are associated with the ratio. One embodiment of a system includes one or more detectors configured to detect spectra for a single event in two or more detection windows. The spectra may include spectra as described above. The one or more detectors are also configured to generate output signals in response to the detected spectra. The system also includes a processor configured to determine a ratio between the output signals and to determine which of the different materials are associated with the ratio.

Abstract translation: 提供了各种计算机实现的方法和系统。 一种计算机实现的方法包括确定通过在两个或更多个检测窗口中检测单个事件的光谱而产生的输出信号之间的比率。 光谱是不同材料的特征。 至少一部分光谱在两个或更多个检测窗口中的至少一个中重叠。 该方法还包括确定哪种不同的材料与该比率相关联。 系统的一个实施例包括一个或多个检测器，其被配置为在两个或更多个检测窗口中检测单个事件的光谱。 光谱可以包括如上所述的光谱。 一个或多个检测器还被配置为响应于检测到的光谱而产生输出信号。 该系统还包括处理器，其被配置为确定输出信号之间的比率并且确定哪个不同的材料与该比率相关联。

公开(公告)号：US06916661B2

公开(公告)日：2005-07-12

申请号：US10694084

申请日：2003-10-28

Applicant: Mark B. Chandler ,  Don J. Chandler

Inventor： Mark B. Chandler ,  Don J. Chandler

IPC: C12Q1/68 ,  G01N15/14 ,  G01N33/00 ,  G01N33/58 ,  G01N33/48 ,  G01N33/536

CPC classification number: G01N33/587 ,  C12Q1/6816 ,  G01N15/14 ,  G01N33/582 ,  Y10S435/81 ,  Y10S435/975 ,  Y10S436/805 ,  Y10S436/808 ,  Y10S436/823 ,  Y10S977/707 ,  Y10S977/773 ,  Y10S977/904 ,  Y10S977/92 ,  Y10T436/10 ,  Y10T436/109163 ,  Y10T436/13 ,  C12Q2563/155 ,  C12Q2563/107 ,  C12Q2537/143 ,  C12Q2565/626

Abstract: This invention provides a novel fluorescent particle including a core or carrier particle having on its surface a plurality of smaller polymeric particles or nanoparticles, which are stained with different fluorescent dyes. When excited by a light source they are capable of giving off multiple fluorescent emissions simultaneously, which is useful for multiplexed analysis of a plurality of analytes in a sample. The coupled complex particles carrying on their surface fluorescent nanoparticles, methods of preparing such polymer particles, and various applications and methods of using such particles are claimed.

公开(公告)号：US06599331B2

公开(公告)日：2003-07-29

申请号：US10185869

申请日：2002-07-01

Applicant: Don J. Chandler ,  Beth A. Lambert ,  Janet J. Reber ,  Stacie L. Phipps

Inventor： Don J. Chandler ,  Beth A. Lambert ,  Janet J. Reber ,  Stacie L. Phipps

IPC: D06P190

CPC classification number: G01N33/533 ,  C12Q1/6816 ,  D06P1/0012 ,  D06P1/922 ,  D06P1/924 ,  D06P1/926 ,  D06P1/928 ,  G01N33/54313 ,  Y10T428/2982 ,  Y10T428/2989 ,  Y10T428/2991 ,  Y10T428/2998

Abstract: An improved method of making a series of bead or microsphere or particle populations characterized by subtle variation in a proportion or ratio of at least two fluorescent dyes distributed within a single bead of each population is provided. These beads, when excited by a single excitation light source are capable of giving off several fluorescent signals simultaneously. A set containing as many as 64 distinct populations of multicolored, fluorescent beads is provided and when combined with analytical reagents bound to the surface of such beads is extremely useful for multiplexed analysis of a plurality of analytes in a single sample. Thus, methods of staining polymeric particles, the particles themselves, and methods of using such particles are claimed.

Abstract translation: 提供了一种制备一系列珠或微球或颗粒群的改进方法，其特征在于分布在每个群体的单个珠粒内的至少两种荧光染料的比例或比例的微小变化。 这些珠当被单个激发光源激发时能够同时发出几个荧光信号。 提供了含有多达64个不同种群的多色荧光珠的组，并且当与结合到这种珠的表面的分析试剂组合时，对于单个样品中的多种分析物的多重分析非常有用。 因此，要求保护染色聚合物颗粒，颗粒本身和使用这些颗粒的方法。

公开(公告)号：US06268222B1

公开(公告)日：2001-07-31

申请号：US09234841

申请日：1999-01-22

Applicant: Mark B. Chandler ,  Don J. Chandler

Inventor： Mark B. Chandler ,  Don J. Chandler

IPC: G01N33536

CPC classification number: G01N33/54346 ,  G01N15/14 ,  G01N33/582 ,  G01N33/587 ,  G01N2015/1018

Abstract: This invention provides a novel fluorescent article comprising a core or carrier particle having on its surface a plurality of smaller polymeric particles or nanoparticles, which are stained with different fluorescent dyes. When excited by a light source they are capable of giving off multiple fluorescent emissions simultaneously, which is useful for multiplexed analysis of a plurality of analytes in a sample. The coupled complex particles carrying on their surface fluorescent nanoparticles, methods of preparing such polymer articles, and various applications and methods of using such particles are claimed.

Abstract translation: 本发明提供了一种新颖的荧光制品，其包含在其表面上具有多个较小的聚合物颗粒或纳米颗粒的芯或载体颗粒，其被不同的荧光染料染色。 当被光源激发时，它们能够同时发出多个荧光发射，这对于样品中多个分析物的多重分析是有用的。 涉及其表面上携带有荧光纳米颗粒的偶联复合颗粒，制备这种聚合物制品的方法以及使用这种颗粒的各种应用和方法。

公开(公告)号：US20100178709A1

公开(公告)日：2010-07-15

申请号：US12731290

申请日：2010-03-25

Applicant: Don J. Chandler ,  Jason Bedre

Inventor： Don J. Chandler ,  Jason Bedre

IPC: G01N21/76 ,  B32B1/00 ,  G01N21/00 ,  H01F1/11

CPC classification number: G01N33/54326 ,  B82Y25/00 ,  C09K11/025 ,  C09K11/06 ,  G01N21/64 ,  G01N21/6428 ,  G01N21/6486 ,  H01F1/0018 ,  H01F1/0036 ,  H01F1/0315 ,  H01F1/063 ,  H01F1/111 ,  H01F1/36 ,  Y10T428/2991 ,  Y10T428/2993 ,  Y10T428/2995 ,  Y10T428/2996 ,  Y10T428/2998 ,  Y10T428/32

Abstract: Microspheres, populations of microspheres, and methods for forming microspheres are provided. One microsphere configured to exhibit fluorescent and magnetic properties includes a core microsphere and a magnetic material coupled to a surface of the core microsphere. About 50% or less of the surface of the core microsphere is covered by the magnetic material. The microsphere also includes a polymer layer surrounding the magnetic material and the core microsphere. One population of microspheres configured to exhibit fluorescent and magnetic properties includes two or more subsets of microspheres. The two or more subsets of microspheres are configured to exhibit different fluorescent and/or magnetic properties. Individual microspheres in the two or more subsets are configured as described above.

Abstract translation: 提供了微球，微球体和形成微球的方法。 配置为显示荧光和磁性的一个微球包括芯微球和耦合到芯微球表面的磁性材料。 核心微球表面的约50％以下被磁性材料覆盖。 微球还包括围绕磁性材料和芯微球的聚合物层。 配置成显示荧光和磁性的一组微球体包括两个或更多个微球子集。 两个或多个微球子集被配置为表现出不同的荧光和/或磁性。 两个或多个子集中的单个微球体如上所述配置。

公开(公告)号：US07445844B2

公开(公告)日：2008-11-04

申请号：US11195185

申请日：2005-08-02

Applicant: Don J. Chandler ,  Van S. Chandler ,  Beth A. Lambert ,  Janet J. Reber ,  Stacie L. Phipps

Inventor： Don J. Chandler ,  Van S. Chandler ,  Beth A. Lambert ,  Janet J. Reber ,  Stacie L. Phipps

IPC: B32B5/16

CPC classification number: G01N33/533 ,  C12Q1/6816 ,  D06P1/0012 ,  D06P1/922 ,  D06P1/924 ,  D06P1/926 ,  D06P1/928 ,  G01N33/54313 ,  Y10T428/2982 ,  Y10T428/2989 ,  Y10T428/2991 ,  Y10T428/2998

Abstract: An improved method of making a series of bead or microsphere or particle populations characterized by subtle variation in a proportion or ratio of at least two fluorescent dyes distributed within a single bead of each population is provided. These beads, when excited by a single excitation light source are capable of giving off several fluorescent signals simultaneously. A set containing as many as 64 distinct populations of multicolored, fluorescent beads is provided and when combined with analytical reagents bound to the surface of such beads is extremely useful for multiplexed analysis of a plurality of analytes in a single sample. Thus, methods of staining polymeric particles, the particles themselves, and methods of using such particles are claimed.

Abstract translation: 提供了一种制备一系列珠或微球或颗粒群的改进方法，其特征在于分布在每个群体的单个珠粒内的至少两种荧光染料的比例或比例的微小变化。 这些珠当被单个激发光源激发时能够同时发出几个荧光信号。 提供了含有多达64个不同种群的多色荧光珠的组，并且当与结合到这种珠的表面的分析试剂组合时，对于单个样品中的多种分析物的多重分析非常有用。 因此，要求保护染色聚合物颗粒，颗粒本身和使用这些颗粒的方法。

公开(公告)号：US06632526B1

公开(公告)日：2003-10-14

申请号：US09172174

申请日：1998-10-14

Applicant: Don J. Chandler ,  Van S. Chandler ,  Beth A. Lambert ,  Janet J. Reber ,  Stacie L. Phipps

Inventor： Don J. Chandler ,  Van S. Chandler ,  Beth A. Lambert ,  Janet J. Reber ,  Stacie L. Phipps

IPC: B32B500

CPC classification number: G01N33/533 ,  C12Q1/6816 ,  D06P1/0012 ,  D06P1/922 ,  D06P1/924 ,  D06P1/926 ,  D06P1/928 ,  G01N33/54313 ,  Y10T428/2982 ,  Y10T428/2989 ,  Y10T428/2991 ,  Y10T428/2998

Abstract: An improved method of making a series of bead or microsphere or particle populations characterized by subtle variation in a proportion or ratio of at least two fluorescent dyes distributed within a single bead of each population is provided. These beads, when excited by a single excitation light source are capable of giving off several fluorescent signals simultaneously. A set containing as many as 64 distinct populations of multicolored, fluorescent beads is provided and when combined with analytical reagents bound to the surface of such beads is extremely useful for multiplexed analysis of a plurality of analytes in a single sample. Thus, methods of staining polymeric particles, the particles themselves, and methods of using such particles are claimed.

Abstract translation: 提供了一种制备一系列珠或微球或颗粒群的改进方法，其特征在于分布在每个群体的单个珠粒内的至少两种荧光染料的比例或比例的微小变化。 这些珠当被单个激发光源激发时能够同时发出几个荧光信号。 提供了含有多达64个不同种群的多色荧光珠的组，并且当与结合到这种珠的表面的分析试剂组合时，对于单个样品中的多种分析物的多重分析非常有用。 因此，要求保护染色聚合物颗粒，颗粒本身和使用这些颗粒的方法的方法