公开(公告)号：US07069152B2

公开(公告)日：2006-06-27

申请号：US10234314

申请日：2002-09-05

申请人： John Skilling

发明人： John Skilling

IPC分类号： C12M1/00 ,  G01N31/48 ,  G01N31/00 ,  G06G7/48

CPC分类号： G01N33/6848 ,  H01J49/0036 ,  H01J49/04

摘要： An apparatus for identifying a protein, polypeptide or peptide by means of mass spectrometry and especially by tandem mass spectrometry is disclosed. The apparatus preferably functions to model the fragmentation of a peptide or protein in a tandem mass spectrometer to facilitate comparison with an experimentally determined spectrum. A fragmentation model is used which takes account of all possible fragmentation pathways which a particular sequence of amino acids may undergo. A peptide or protein may be identified by comparing an experimentally determined mass spectrum with spectra predicted using such a fragmentation model from a library of known peptides or proteins.

摘要翻译： 公开了一种用于通过质谱法，特别是通过串联质谱鉴定蛋白质，多肽或肽的装置。 该装置优选用于在串联质谱仪中模拟肽或蛋白质的断裂以便于与实验确定的光谱进行比较。 考虑到氨基酸的特定序列可能经历的所有可能的断裂途径被使用的碎片模型。 肽或蛋白质可以通过将实验确定的质谱与使用已知肽或蛋白质文库的这种断裂模型预测的光谱进行比较来鉴定。