公开(公告)号：WO2010037105A1

公开(公告)日：2010-04-01

申请号：PCT/US2009/058815

申请日：2009-09-29

Applicant: BUTAMAX™ ADVANCED BIOFUELS LLC ,  PAUL, Brian, James ,  SUH, Wonchul

Inventor： PAUL, Brian, James ,  SUH, Wonchul

IPC: C12N9/04 ,  C12N15/74 ,  C12N9/88 ,  C12P7/16 ,  C12R1/225 ,  C12R1/46

CPC classification number: C12N9/88 ,  C12P7/16 ,  Y02E50/10

Abstract: Lactic acid bacterial (LAB) cells were modified such that they have a specific activity of dihydroxy-acid dehydratase enzyme activity that is increased to about 0.1 µmol min -1 mg -1 . LAB cells with even higher activities of 0.2 to 0.6 µmol min -1 mg -1 of DHAD activity were obtained. These modified cells may be used to produce isobutanol when additional isobutanol biosynthetic pathway enzymes are expressed.

Abstract translation: 改变乳酸菌（LAB）细胞使其具有增加至约0.1μmol/ min-1mg-1的二羟基酸脱水酶活性的比活性。 获得具有0.2至0.6μmol/ min-1mg-1的DHAD活性的更高活性的LAB细胞。 当额外的异丁醇生物合成途径酶被表达时，这些修饰的细胞可用于产生异丁醇。

公开(公告)号：WO2010037119A1

公开(公告)日：2010-04-01

申请号：PCT/US2009/058843

申请日：2009-09-29

Applicant: BUTAMAX™ ADVANCED BIOFUELS LLC ,  PAUL, Brian, James ,  SUH, Wonchul

Inventor： PAUL, Brian, James ,  SUH, Wonchul

IPC: C12N9/88 ,  C12P7/16 ,  C12R1/46 ,  C12R1/225

CPC classification number: C12N9/88 ,  C12P7/16 ,  Y02E50/10

Abstract: Lactic acid bacteria expressing dihydroxyacid dehydratase polypeptides with increased specific activity are disclosed. The lactic acid bacteria comprise recombinant genes encoding iron-sulfur cluster forming proteins.

Abstract translation: 公开了具有增加的比活性的表达二羟基酸脱水酶多肽的乳酸菌。 乳酸菌包括编码铁硫簇形成蛋白的重组基因。

公开(公告)号：WO2010037112A1

公开(公告)日：2010-04-01

申请号：PCT/US2009/058827

申请日：2009-09-29

Applicant: BUTAMAX™ ADVANCED BIOFUELS LLC ,  FLINT, Dennis ,  ROTHMAN, Steven, Cary ,  SUH, Wonchul ,  TOMB, Jean-Francois ,  YE, Rick, W.

Inventor： FLINT, Dennis ,  ROTHMAN, Steven, Cary ,  SUH, Wonchul ,  TOMB, Jean-Francois ,  YE, Rick, W.

IPC: C12N9/88 ,  C12P7/16 ,  C12P13/06 ,  C12P13/08 ,  C12P7/40

CPC classification number: G06F19/22 ,  C12N9/88 ,  C12P7/16 ,  C12P7/40 ,  C12P13/06 ,  C12P13/08 ,  C12Y402/01009 ,  Y02E50/10

Abstract: A group of bacterial dihydroxy-acid dehydratases having a [2Fe-2S] cluster was discovered. Bacterial [2Fe-2S] DHADs were expressed as heterologous proteins in bacteria and yeast cells, providing DHAD activity for conversion of 2,3-dihydroxyisovalerate to α-ketoisovalerate or 2,3-dihydroxymethylvalerate to α-ketomethylvalerate. Isobutanol and other compounds may be synthesized in pathways that include bacterial [2Fe-2S] DHAD activity.

Abstract translation: 发现一组具有[2Fe-2S]簇的细菌二羟基酸脱水酶。 细菌[2Fe-2S] DHADs在细菌和酵母细胞中被表达为异源蛋白质，为2,3-二羟基异戊酸转化为α-酮异戊酸酯或2,3-二羟甲基戊酸酯转化为α-酮戊二酸酯提供了DHAD活性。 异丁醇和其他化合物可以在包括细菌[2Fe-2S] DHAD活性的途径中合成。

公开(公告)号：WO2010099524A1

公开(公告)日：2010-09-02

申请号：PCT/US2010/025735

申请日：2010-03-01

Applicant: BUTAMAX™ ADVANCED BIOFUELS LLC ,  VAN DYK, Tina, K.

Inventor： VAN DYK, Tina, K.

IPC: C12N1/16 ,  C12N1/18 ,  C12P7/16

CPC classification number: C12P7/16 ,  Y02E50/10

Abstract: Increasing tolerance to butanol in yeast has been accomplished by decreasing activity of Pdr5p encoded by an endogenous PDR5 gene. A deletion mutation of the PDR5 gene led to improved growth yield in the presence of butanol. Yeast cells with reduced Pdr5p activity, or other multidrug resistance ATP-binding cassette transporter protein activity encoded by CDR1 or BFR1, and a butanol biosynthetic pathway may be used for improved butanol production

Abstract translation: 通过降低由内源性PDR5基因编码的Pdr5p的活性，可以增加对酵母中丁醇的耐受性。 PDR5基因的缺失突变导致丁醇存在下生长产量提高。 具有降低的Pdr5p活性的酵母细胞或由CDR1或BFR1编码的其他多药耐药性ATP结合盒转运蛋白活性和丁醇生物合成途径可用于改善丁醇生产

公开(公告)号：WO2010091069A1

公开(公告)日：2010-08-12

申请号：PCT/US2010/023027

申请日：2010-02-03

Applicant: BUTAMAX™ ADVANCED BIOFUELS LLC ,  WOLF, Leslie, R.

Inventor： WOLF, Leslie, R.

IPC: C10L1/22 ,  C10L1/228 ,  C10L1/222 ,  C10L10/18 ,  C10L1/2383 ,  C10L1/2387 ,  C10L1/232 ,  C08F8/30 ,  C08G59/14 ,  C07C209/78 ,  C07C223/00 ,  C07C249/02

CPC classification number: C10L1/22 ,  C07C249/02 ,  C10L1/026 ,  C10L1/1824 ,  C10L1/221 ,  C10L1/2222 ,  C10L1/224 ,  C10L1/226 ,  C10L1/228 ,  C10L1/2283 ,  C10L1/232 ,  C10L1/238 ,  C10L1/2383 ,  C10L1/2387 ,  C10L10/18

Abstract: [0070] A gasoline deposit control additive composition for use in a fuel comprising from about 70 to about 95 volume percent of hydrocarbons in the gasoline boiling range and from about 5 to about 30 volume percent of at least one alcohol, comprising the imine or tertiary amine product of the reaction between (a) at least one aldehyde or ketone or mixture thereof having the formula R 16 CHO, R 16 CH 2 CHO, R 17 (C=O) R 18 or R 17 CH 2 (C=O) R 18 , wherein R 16 , R 17 , and R 18 are the same or different and are each independently a straight or branched chain hydrocarbyl or aryl group that contains from 1 to 18 carbon atoms, and (b) a primary or secondary amine functionality.

Abstract translation: 一种用于燃料的汽油沉积物控制添加剂组合物，其包含约70至约95体积％的汽油沸程中的烃和约5至约30体积％的至少一种醇，其包含亚胺或三级 （a）至少一种具有式R 16 CHO，R 16 CH 2 CHO，R 17（C = O）R 18或R 17 CH 2（C = O）R 18的醛或酮或其混合物之间的反应的胺产物，其中R 16，R 17， 和R 18相同或不同，并且各自独立地为含有1至18个碳原子的直链或支链烃基或芳基，和（b）伯胺或仲胺官能团。

公开(公告)号：WO2010037114A1

公开(公告)日：2010-04-01

申请号：PCT/US2009/058834

申请日：2009-09-29

Applicant: BUTAMAX™ ADVANCED BIOFUELS LLC ,  PAUL, Brian, James

Inventor： PAUL, Brian, James

IPC: C12R1/225 ,  C12R1/46 ,  C12N9/04 ,  C12P7/16

CPC classification number: C12N15/746 ,  C12N9/0006 ,  C12P7/16 ,  C12P7/18 ,  C12P7/26 ,  C12Y101/02004 ,  Y02E50/10

Abstract: A high flux of metabolites from pyruvate to 2,3-butanediol in Lactobacillus plantarum was achieved through genetic engineering. Substantial elimination of lactate dehydrogenase activity in the presence of heterologously expressed butanediol dehydrogenase activity led to 2,3 butanediol production that was at least 49% of the total of major pyruvate-derived products.

Abstract translation: 通过基因工程实现了植物乳杆菌中从丙酮酸到2,3-丁二醇的高通量代谢物。 在异源表达的丁二醇脱氢酶活性的存在下，大量消除乳酸脱氢酶活性导致2,3-丁二醇的产生，其至少是丙酮酸衍生产物总数的49％。

公开(公告)号：WO2010119339A3

公开(公告)日：2010-10-21

申请号：PCT/IB2010/000966

申请日：2010-04-13

Applicant: BUTAMAX™ ADVANCED BIOFUELS LLC ,  GRADY, Michael, Charles ,  HALLAM, John, W. ,  JAHIC, Mehmedalija ,  PATNAIK, Ranjan

Inventor： GRADY, Michael, Charles ,  HALLAM, John, W. ,  JAHIC, Mehmedalija ,  PATNAIK, Ranjan

IPC: C12P7/16

Abstract: A method for producing butanol through microbial fermentation, in which the butanol product is removed by extraction into a water-immiscible extractant composition comprising a first solvent and a second solvent, is provided. The first solvent is selected from the group consisting of C 12 to C 22 fatty alcohols, C 12 to C 22 fatty acids, esters of C 12 to C 22 fatty acids, C 12 to C 22 fatty aldehydes, C 12 to C 22 fatty amides and mixtures thereof. The second solvent is selected from the group consisting of C 7 to C 11 alcohols, C 7 to C 11 carboxylic acids, esters of C 7 to C 11 carboxylic acids, C 7 to C 11 aldehydes, and mixtures thereof. Also provided is a method for recovering butanol from a fermentation medium.

公开(公告)号：WO2010037111A1

公开(公告)日：2010-04-01

申请号：PCT/US2009/058826

申请日：2009-09-29

Applicant: BUTAMAX™ ADVANCED BIOFUELS LLC ,  ANTHONY, Larry, Cameron ,  MAGGIO-HALL, Lori Ann ,  ROTHMAN, Steven, Cary ,  TOMB, Jean-Francois

Inventor： ANTHONY, Larry, Cameron ,  MAGGIO-HALL, Lori Ann ,  ROTHMAN, Steven, Cary ,  TOMB, Jean-Francois

IPC: C12P7/16 ,  C12N9/88 ,  C12N15/09 ,  C12R1/645

CPC classification number: C12P7/40 ,  C12N9/88 ,  C12P7/16 ,  C12P7/26 ,  C12Y402/01009 ,  Y02E50/10

Abstract: Yeast strains were engineered that have increased activity of heterologous proteins that require binding of an Fe-S cluster for their activity. The yeast strains have reduced activity of an endogenous Fe-S protein. Activities of heterologous fungal or plant 2Fe-2S dihydroxy-acid dehydratases and Fe-S propanediol dehydratase reactivase were increased for increased production of products made using biosynthetic pathways including these enzymes, such as valine, isoleucine, leucine, pantothenic acid (vitamin B5), isobutanol, 2-butanone and 2-butanol.

Abstract translation: 工程化的酵母菌株具有增加Fe-S簇结合活性的异源蛋白质的活性。 酵母菌株内源性Fe-S蛋白的活性降低。 增加异源真菌或植物2Fe-2S二羟基酸脱水酶和Fe-S丙二醇脱水酶再活化酶的活性，以增加使用包括这些酶的生物合成途径（如缬氨酸，异亮氨酸，亮氨酸，泛酸（维生素B5））， 异丁醇，2-丁酮和2-丁醇。

公开(公告)号：WO2010062597A1

公开(公告)日：2010-06-03

申请号：PCT/US2009/062207

申请日：2009-10-27

Applicant: BUTAMAX™ ADVANCED BIOFUELS LLC ,  ANTHONY, Larry, Cameron ,  DAUNER, Michael ,  DONALDSON, Gail, K. ,  PAUL, Brian, James

Inventor： ANTHONY, Larry, Cameron ,  DAUNER, Michael ,  DONALDSON, Gail, K. ,  PAUL, Brian, James

IPC: C12P7/16 ,  C12N1/16 ,  C12N1/21 ,  C12N9/04 ,  C12N9/12 ,  C12N9/18 ,  C12N9/88 ,  C12N9/92

CPC classification number: C12P7/16 ,  C12N9/0006 ,  C12N9/1205 ,  C12N9/18 ,  C12N9/88 ,  C12N9/92 ,  Y02E50/10

Abstract: A microbial host cell is provided for the production of isobutanol. Carbon flux in the cell is optimized through the Entner-Doudorof pathway.

Abstract translation: 提供用于生产异丁醇的微生物宿主细胞。 通过Entner-Doudorof途径优化细胞中的碳通量。