公开(公告)号：WO1989004366A1

公开(公告)日：1989-05-18

申请号：PCT/US1988003857

申请日：1988-10-31

Applicant: GROB, Howard, S. ,  FRIEDMAN, Frank

IPC: C12N05/00

CPC classification number: C12N5/0609 ,  C12N2500/40 ,  C12N2501/31 ,  C12N2501/33 ,  C12N2501/395 ,  C12N2501/81 ,  C12N2517/10

Abstract: A method is disclosed for the in vitro growth and development of cells obtained from the follicles of a mammal to reproductively competent oocytes. By incubating these cells, which comprise small and medium oocytes, in a series of specific hormone-supplemented tissue culture media as described herein, they can be induced to grow and develop into reproductively competent oocytes. After a further maturation step in which chromosome number reduction occurs, the oocytes are fertilizable.