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    METHODS FOR THE COLLECTION AND MATURATION OF OOCYTES
    2.
    发明申请
    METHODS FOR THE COLLECTION AND MATURATION OF OOCYTES 审中-公开
    方法收集和成熟的OOCYTES

    公开(公告)号：WO2010130008A1

    公开(公告)日：2010-11-18

    申请号：PCT/AU2010/000569

    申请日：2010-05-14

    Applicant: ADELAIDE RESEARCH & INNOVATION PTY LTD ,  GILCHRIST, Robert, Bruce ,  THOMPSON, Jeremy ,  ALBUZ, Firas

    Inventor： GILCHRIST, Robert, Bruce ,  THOMPSON, Jeremy ,  ALBUZ, Firas

    IPC: C12N5/075 ,  C12N5/02

    CPC classification number: C12N5/0609 ,  A61K38/1808 ,  A61K38/24 ,  C12N15/873 ,  C12N2501/01 ,  C12N2501/11 ,  C12N2501/31 ,  C12N2517/10

    Abstract: The present invention relates to a method of producing an embryo from an oocyte by an assisted reproduction technology. The method includes (a) collecting an oocyte from an ovary of a subject in a collection medium comprising a first phosphodiesterase inhibitor and an agent that increases intracellular cAMP concentration in the oocyte, (b) culturing the oocyte in a maturation medium comprising a second phosphodiesterase inhibitor, and (c) producing an embryo from the oocyte by an assisted reproduction technology. The present invention also relates to methods of inducing oocyte maturation. For example a method of in vitro maturation of an oocyte is described which comprises steps (a) and (b) above. The present invention also relates to an oocyte maturation medium comprising a phosphodiesterase inhibitor and a ligand for inducing maturation of the oocyte. A combination product comprising an oocyte collection and maturation medium referred to above is also described.

    Abstract translation: 本发明涉及通过辅助再现技术从卵母细胞产生胚胎的方法。 该方法包括（a）在收集介质中收集卵母细胞，所述收集培养基包含第一磷酸二酯酶抑制剂和增加卵母细胞中细胞内cAMP浓度的试剂，（b）在包含第二磷酸二酯酶的成熟培养基中培养卵母细胞 抑制剂，和（c）通过辅助再生技术从卵母细胞产生胚胎。 本发明还涉及诱导卵母细胞成熟的方法。 例如，描述了包含上述步骤（a）和（b）的卵母细胞的体外成熟方法。 本发明还涉及包含磷酸二酯酶抑制剂和诱导卵母细胞成熟的配体的卵母细胞成熟培养基。 还描述了包含上文所述的卵母细胞收集和成熟培养基的组合产品。

    METHODS AND SYSTEMS FOR THE PRODUCTION OF GRANULOSA CELLS
    3.
    发明申请
    METHODS AND SYSTEMS FOR THE PRODUCTION OF GRANULOSA CELLS 审中-公开
    用于生产颗粒细胞的方法和系统

    公开(公告)号：WO2009138855A3

    公开(公告)日：2010-05-20

    申请号：PCT/IB2009005577

    申请日：2009-05-12

    Applicant: UNIVERSITAETSSPITAL BASEL ,  DE GEYTER CHRISTIAN ,  SCHERBERICH ARNAUD ,  KOSSOWSKA KATARZYNA

    Inventor： DE GEYTER CHRISTIAN ,  SCHERBERICH ARNAUD ,  KOSSOWSKA KATARZYNA

    IPC: C12N5/071

    CPC classification number: C12N5/0682 ,  C12N5/0607 ,  C12N2501/235 ,  C12N2501/31

    Abstract: The present invention concerns methods and related cultured granulosa cells, assays and kits for drug discovery, therapeutic, and diagnostic purposes based on the discovery that granulosa cells cultured as monolayers remain viable in vitro over prolonged time periods, and exhibit stem cell potential when supplemented with leukaemia-inhibiting factor (LIF), and that use of a three-dimensional culture system such as type I collagen together with the use of LIF allows for both the survival and growth of preantral human GC while supporting a significant subpopulation of GC to maintain their characteristics for prolonged time periods, such as their ability to produce follicle-stimulating hormone receptor and cytochrome P450 aromatase.

    Abstract translation: 本发明涉及方法和相关的培养颗粒细胞，用于药物发现，治疗和诊断目的的测定和试剂盒，其基于以下发现：以单层培养的颗粒细胞在延长的时间段内在体外保持活性，并且当补充有 白血病抑制因子（LIF），并且使用三维培养系统如I型胶原蛋白以及LIF的使用允许前人类GC的存活和生长，同时支持GC的重要亚群以维持其 延长时间的特征，例如其产生促卵泡激素受体和细胞色素P450芳香酶的能力。

    METHOD TO DECREASE THE RATE OF POLYSPERMY IN IVF
    4.
    发明申请
    METHOD TO DECREASE THE RATE OF POLYSPERMY IN IVF 审中-公开
    降低体外受精率的方法

    公开(公告)号：WO2006012177A2

    公开(公告)日：2006-02-02

    申请号：PCT/US2005022180

    申请日：2005-06-23

    Applicant: UNIV MISSOURI ,  PRATHER RANDALL S

    Inventor： PRATHER RANDALL S

    IPC: A01K67/027

    CPC classification number: C12N15/873 ,  A01K2227/108 ,  C12N5/0609 ,  C12N5/061 ,  C12N15/8778 ,  C12N2501/11 ,  C12N2501/20 ,  C12N2501/31 ,  C12N2501/58 ,  C12N2501/998 ,  C12N2517/10

    Abstract: The field of invention generally relates to increasing the efficiency of in vitro fertilization by decreasing the rate of polyspermy. One aspect of the invention provides a method of reducing polyspermy in in vitro fertilization by forming an in vitro fertilization mixture that contains osteopontin, oocytes, and sperm, and allowing fertilization of the oocyte by sperm. Another aspect of the invention provides an aqueous mixture for in vitro fertilization that contains osteopontin, oocytes, and sperm.

    Abstract translation: 本发明的领域通常涉及通过降低多糖的速率来提高体外受精的效率。 本发明的一个方面提供了通过形成含有骨桥蛋白，卵母细胞和精子的体外受精混合物并且允许精子对卵母细胞进行受精来减少体外受精中的多糖的方法。 本发明的另一方面提供了含有骨桥蛋白，卵母细胞和精子的体外受精的含水混合物。

    HORMONE-SECRETING CELLS MAINTAINED IN LONG-TERM CULTURE
    5.
    发明申请
    HORMONE-SECRETING CELLS MAINTAINED IN LONG-TERM CULTURE 审中-公开
    长期保存的细胞分泌细胞

    公开(公告)号：WO1993000441A1

    公开(公告)日：1993-01-07

    申请号：PCT/US1992005267

    申请日：1992-06-23

    Applicant: PACIFIC BIOMEDICAL RESEARCH, INC. ,  BROTHERS, Ann, Janice

    Inventor： PACIFIC BIOMEDICAL RESEARCH, INC.

    IPC: C12P19/00

    CPC classification number: C12N5/0682 ,  A61K38/00 ,  C12N5/0605 ,  C12N5/0617 ,  C12N5/0676 ,  C12N2500/32 ,  C12N2500/33 ,  C12N2500/34 ,  C12N2500/36 ,  C12N2500/38 ,  C12N2501/31 ,  C12N2503/00 ,  C12P21/02 ,  G01N33/5005 ,  G01N2800/52

    Abstract: Methods are provided for the establishment and maintenance in long term culture of hormone secreting cells. Cells are derived from tumorous or non-tumorous animal or human tissues, including ovary, endometrium, trophoblast, pituitary, thyroid, and pancreas. The cells secrete into the culture medium hormones such as estrogens, progestins, follicle-stimulating hormone, luteinizing hormone, human chorionic gonadotrophin, thyroxin, glucagon, and insulin, depending on the tissue of origin of individual cell cultures. Contact with an appropriate secretogogue causes the cells to respond with increased hormone secretion. For instance, ovarian follicular cells respond to follicle-stimulating hormone with increased estrogen and progesterone secretion. Pancreatic cells respond to elevated glucose with increased insulin secretion. The cells proliferate in in vitro for up to one year or longer, during which time they retain their hormone-secretion profile. The cells may be frozen for storage, and retain their hormone-secretion profile after thawing. The cell cultures are useful for the production of human hormones, for the bio-assay of drugs such as therapeutic gonadotrophins, for the testing of drug efficacy and design, and for toxicity testing of drugs and chemicals. The cells may also be implanted in an individual to replace deficient hormone secretion. For instance, insulin secreting pancreatic cells may be implanted in a diabetic individual as an adjunct or replacement therapy for exogenously administered insulin.

    Abstract translation: 提供了用于在激素分泌细胞的长期培养中建立和维持的方法。 细胞衍生自肿瘤或非肿瘤的动物或人体组织，包括卵巢，子宫内膜，滋养层，垂体，甲状腺和胰腺。 取决于个体细胞培养物的起源组织，细胞分泌到培养基激素如雌激素，孕激素，促卵泡激素，促黄体激素，人绒毛膜促性腺激素，甲状腺素，胰高血糖素和胰岛素。 与适当的分泌物接触导致细胞反应增加激素分泌。 例如，卵巢滤泡细胞对增加雌激素和孕激素分泌的促卵泡激素有反应。 胰岛素分泌增加胰腺细胞对葡萄糖升高反应。 细胞在体外增殖长达一年或更长时间，在此期间它们保留了激素分泌特征。 细胞可以冷冻储存，并在解冻后保留其激素分泌曲线。 细胞培养物可用于生产人类激素，用于药物如治疗性促性腺激素的生物测定，用于药物疗效和设计的测试以及药物和化学品的毒性测试。 细胞也可以植入个体以代替缺乏激素分泌。 例如，胰岛素分泌胰腺细胞可以植入糖尿病个体作为外源性给药胰岛素的辅助或替代疗法。

    IN VITRO MATURATION OF BOVINE OOCYTES
    6.
    发明申请
    IN VITRO MATURATION OF BOVINE OOCYTES 审中-公开
    牛肉蛋白的体外成熟

    公开(公告)号：WO1990013627A1

    公开(公告)日：1990-11-15

    申请号：PCT/US1990002365

    申请日：1990-04-27

    Applicant: GRANADA BIOSCIENCES, INC.

    Inventor： GRANADA BIOSCIENCES, INC. ,  BARNES, Frank, L. ,  WESTHUSIN, Mark, E.

    IPC: C12N05/06

    CPC classification number: A01K67/02 ,  C12N5/0609 ,  C12N2501/31 ,  C12N2517/10

    Abstract: The method of this invention produces mature oocytes from culturing immature oocytes in a maturation media and co-culture which have developmental competence and can be used for nuclear transfer. The maturation media can contain recombinant gonadotropins. The co-culture is media with bovine oviductal cells.

    Abstract translation: 本发明的方法产生成熟的卵母细胞，其在成熟培养基和共培养物中培养未成熟的卵母细胞，其具有发育能力并可用于核转移。 成熟培养基可以含有重组促性腺激素。 共培养是具有牛输卵管细胞的培养基。

    肝細胞誘導方法
    7.
    发明申请
    肝細胞誘導方法 审中-公开
    HEPATOCYTE感应方法

    公开(公告)号：WO2016104717A1

    公开(公告)日：2016-06-30

    申请号：PCT/JP2015/086260

    申请日：2015-12-25

    Applicant: 国立大学法人京都大学

    Inventor： 長船　健二 ,  小高　真希

    IPC: C12N5/071 ,  C12N5/0735 ,  C12N5/10 ,  C12Q1/02 ,  G01N33/50

    CPC classification number: C12N5/067 ,  C12N5/10 ,  C12N2500/30 ,  C12N2501/31 ,  C12N2501/39 ,  C12N2501/727 ,  C12N2501/73 ,  C12N2501/80 ,  C12N2506/45 ,  C12Q1/02 ,  G01N33/50 ,  G01N33/5067

    Abstract: 　肝芽細胞から肝細胞を製造する方法を提供する。　肝芽細胞をプレグネノロンおよびアドレナリン作動薬から成る群より選択された薬剤を含む培地中で培養する工程を含む、肝芽細胞から肝細胞の製造方法を提供する。肝芽細胞は、内胚葉細胞をDMSOを含む培地中で培養することによって得ることができ、内胚葉細胞は多能性幹細胞を、Activin AおよびGSK-3β阻害剤を含む培地で培養ことによって得ることができる。従って、本発明の方法を用いることで、多能性幹細胞から肝細胞を製造する方法も合わせて提供し得る。

    Abstract translation: 提供一种诱导成肝细胞肝细胞的方法。 提供了一种从成肝细胞诱导肝细胞的方法，所述方法包括将成肝细胞培养在含有选自由孕烯醇酮和肾上腺素能激动剂组成的组的化学物质的培养基中的步骤。 可以通过在含有DMSO的培养基中培养内胚层细胞获得成肝细胞，并且可以通过在含有激活素A和GSK-3β抑制剂的培养基中培养多能干细胞来获得内胚层细胞。 因此，通过采用本发明的方法，也可以提供从多能干细胞产生肝细胞的方法。

    METHOD OF IN VITRO FERTILIZATION WITH DELAY OF EMBRYO TRANSFER AND USE OF PERIPHERAL BLOOD MONONUCLEAR CELLS
    9.
    发明申请
    METHOD OF IN VITRO FERTILIZATION WITH DELAY OF EMBRYO TRANSFER AND USE OF PERIPHERAL BLOOD MONONUCLEAR CELLS 审中-公开
    用于延长胚胎移植和外周血单核细胞的使用的方法

    公开(公告)号：WO2013078312A1

    公开(公告)日：2013-05-30

    申请号：PCT/US2012/066258

    申请日：2012-11-21

    Applicant: MEZADATA MEDICAL IP HOLDING LLP

    Inventor： FESKOV, Alexander ,  FESKOVA, Irina ,  ZHYLKOVA, Ievgeniia ,  ZHILKOV, Stanislav

    IPC: C12N5/00

    CPC classification number: A61B17/435 ,  A61D19/00 ,  A61D19/04 ,  A61K35/14 ,  A61K2035/124 ,  C12N5/0634 ,  C12N2501/31

    Abstract: A method of in vitro fertilization wherein the embryo is implanted into the uterus of a female patient at least two, and preferably three to twelve months after the eggs are retrieved from the patient in order to reduce the effect of autoimmune rejection of the embryo by the patient's autoimmune system and increase the probability and success of pregnancy and wherein prior to embryo implantation, the endometrium in the uterus is prepared for embryo implantation by introducing peripheral blood mononuclear cells (PBMCs) into the uterus. The procedure is combined with cryopreservation techniques to preserve the oocytes or the IVF-produced embryos of the patient.

    Abstract translation: 一种体外受精的方法，其中将胚胎植入女性患者的子宫中，在从患者中取出卵后至少两个，优选三至十二个月，以便通过所述方法减少胚胎的自身免疫排斥的影响 患者的自身免疫系统，并增加妊娠的概率和成功率，并且在胚胎植入之前，通过将外周血单核细胞（PBMC）引入子宫来准备子宫内膜的胚胎植入。 该方法与冷冻保存技术结合，以保存患者的卵母细胞或IVF产生的胚胎。

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