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    METHOD FOR IN VITRO MATURATION OF OOCYTES
    2.
    发明申请
    METHOD FOR IN VITRO MATURATION OF OOCYTES 审中-公开
    用于卵巢成纤维细胞生长的方法

    公开(公告)号：WO1989004366A1

    公开(公告)日：1989-05-18

    申请号：PCT/US1988003857

    申请日：1988-10-31

    Applicant: GROB, Howard, S. ,  FRIEDMAN, Frank

    IPC: C12N05/00

    CPC classification number: C12N5/0609 ,  C12N2500/40 ,  C12N2501/31 ,  C12N2501/33 ,  C12N2501/395 ,  C12N2501/81 ,  C12N2517/10

    Abstract: A method is disclosed for the in vitro growth and development of cells obtained from the follicles of a mammal to reproductively competent oocytes. By incubating these cells, which comprise small and medium oocytes, in a series of specific hormone-supplemented tissue culture media as described herein, they can be induced to grow and develop into reproductively competent oocytes. After a further maturation step in which chromosome number reduction occurs, the oocytes are fertilizable.

    <i>IN VITRO</i> CELL CULTURE DEVICE INCLUDING CARTILAGE AND METHODS OF USING THE SAME
    4.
    发明申请
    IN VITRO CELL CULTURE DEVICE INCLUDING CARTILAGE AND METHODS OF USING THE SAME 审中-公开
    细胞培养装置中的细胞培养装置及其使用方法

    公开(公告)号：WO0073417A9

    公开(公告)日：2002-04-18

    申请号：PCT/US0014526

    申请日：2000-05-26

    Applicant: UNIV NEW YORK STATE RES FOUND

    Inventor： HICKS WESLEY L JR

    IPC: A61K35/12 ,  C12M1/22 ,  C12N5/00 ,  C12N5/02 ,  C12N5/071 ,  G01N33/50 ,  C12M3/00

    CPC classification number: G01N33/5044 ,  A61K35/12 ,  C12M23/10 ,  C12M23/20 ,  C12M23/22 ,  C12M25/06 ,  C12M35/08 ,  C12N5/0068 ,  C12N5/0688 ,  C12N5/0697 ,  C12N2500/38 ,  C12N2501/11 ,  C12N2501/33 ,  C12N2501/39 ,  C12N2501/81 ,  C12N2502/1317 ,  C12N2502/27 ,  C12N2503/04 ,  C12N2533/54 ,  G01N33/5008 ,  G01N33/502 ,  G01N33/5029 ,  G01N33/5061 ,  G01N33/5064

    Abstract: The present invention relates to an in vitro cell culture device which includes a vessel comprising an inner surface, a layer of cartilage disposed on at least a portion of said inner surface, the layer of cartilage including a plurality of chondrocytes in an extracellular matrix, and a growth medium in the vessel, the layer of cartilage being bathed in the growth medium. Also disclosed is a composite cell culture prepared from the in vitro cell culture device, the composite cell culture inludes a first layer including chondrocytes in an extracellular matrix, a second layer disposed on the first layer and including type I collagen, and a third layer disposed on the second layer and including cells at least partially covering the second layer. Further aspects of the present invention relate to methods of preparing an in vitro composite cell culture, methods of screening putative therapeutic agents for activity in promoting re-epithelialization of cartilaginous tissues, and methods of screening putative therapeutic agents for activity in inhibiting growth factors or proteinases.

    Abstract translation: 本发明涉及一种体外细胞培养装置，其包括容器，该容器包括内表面，设置在所述内表面的至少一部分上的软骨层，所述软骨层包括多个 细胞外基质中的软骨细胞和血管中的生长培养基，软骨层被沐浴在生长培养基中。 还公开了从体外细胞培养装置制备的复合细胞培养物，复合细胞培养物包括在细胞外基质中包括软骨细胞的第一层，设置在第一层上的第二层，包括I型 胶原蛋白和设置在第二层上的第三层，并且包括至少部分覆盖第二层的细胞。 本发明的其它方面涉及制备体外复合细胞培养物的方法，筛选用于促进软骨组织再上皮化的活性的推定治疗剂的方法，以及筛选推定的治疗剂的方法 抑制生长因子或蛋白酶的活性。

    METHOD FOR PRODUCING ADIPOCYTES FROM NON-DIFFERENTIATED FIBROBLASTS AND USE OF RESULTING ADIPOCYTES
    5.
    发明申请
    METHOD FOR PRODUCING ADIPOCYTES FROM NON-DIFFERENTIATED FIBROBLASTS AND USE OF RESULTING ADIPOCYTES 审中-公开
    生产方法脂肪细胞分化从非成纤维细胞区别对待的使用方法得到的脂肪细胞

    公开(公告)号：WO00037607A3

    公开(公告)日：2000-11-09

    申请号：PCT/FR1999/003174

    申请日：1999-12-17

    IPC: C12N5/02 ,  C12N5/077 ,  C12N5/08 ,  G01N33/50

    CPC classification number: C12N5/0653 ,  C12N2500/38 ,  C12N2501/01 ,  C12N2501/33 ,  C12N2501/39 ,  C12N2501/395 ,  C12N2501/81 ,  C12N2501/999 ,  C12N2510/00

    Abstract: The invention concerns a method for producing adipocytes from non-differentiated fibroblasts characterised in that it consists in culturing non-differentiated fibroblasts having reached confluency and expressing in stable manner the human beta 3 adrenergic receptor or the human beta 3 adrenergic receptor mutated W64R, in a medium comprising agents stimulating differentiation of the adipocytes.

    Abstract translation: 本发明涉及一种方法，用于从分化的成纤维细胞，其特征在于不通过培养未分化的成纤维细胞达到汇合和稳定表达人肾上腺素能β3受体或人β3肾上腺素能受体突变W64R产生脂肪细胞 在包含刺激脂肪细胞分化的试剂的培养基中。

    INJECTABLE MICROTISSUE SYSTEMS, DEVICES, AND METHODS
    6.
    发明申请
    INJECTABLE MICROTISSUE SYSTEMS, DEVICES, AND METHODS 审中-公开
    可注意的微观系统，设备和方法

    公开(公告)号：WO2016140716A1

    公开(公告)日：2016-09-09

    申请号：PCT/US2015/063266

    申请日：2015-12-01

    Applicant: THE TRUSTEES OF COLUMBIA UNIVERSITY IN THE CITY OF NEW YORK

    Inventor： ROSSEN, Ninna, S. ,  SIA, Samuel, K. ,  GILLETTE, Brian, M.

    IPC: A61P3/04 ,  C12N5/074 ,  C12N5/0775

    CPC classification number: A61K35/44 ,  A61K9/0019 ,  A61K9/5063 ,  A61K35/28 ,  C12N5/0691 ,  C12N5/0697 ,  C12N2501/02 ,  C12N2501/115 ,  C12N2501/165 ,  C12N2501/33 ,  C12N2501/385 ,  C12N2501/39 ,  C12N2501/395 ,  C12N2501/81 ,  C12N2502/1352 ,  C12N2502/1358 ,  C12N2502/28 ,  C12N2539/00

    Abstract: Spheroid microtissues can mimic native tissue-like structure and function, and are thus useful for broad research applications and regenerative medicine. Disclosed embodiments include spheroid production methods that are high-throughput, suitable for efficient production, maintainable over long-term culture, and/or offer repeatable control over size distribution. Embodiments disclosed include spheroids that include blood vessels, which are proven useful for tissue survival and function. Embodiments include spheroids with functional, blood-perfused vascular networks upon injection in vivo. Dissolvable hydrogel microwell arrays enable high throughput parallel formation of spheroids in a single pipetting step and easy retrieval for downstream applications. Dynamic culture conditions stimulated endothelial cell and mesenchymal stem cell cocultures to self-organize and form blood-vessel structures within the spheroids. Vascularized spheroids were injected into mice and rapid perfusion of implant vessels within 4-7 days were observed.

    Abstract translation: 球状微结构可以模拟天然组织样结构和功能，因此可用于广泛的研究应用和再生医学。 公开的实施例包括高通量的球形生产方法，适合于有效生产，可长期培养可维持，和/或提供可重复控制的尺寸分布。 公开的实施方案包括包括血管的球体，其被证明可用于组织存活和功能。 实施方案包括在体内注射时具有功能性，血液灌注血管网络的球体。 可溶性水凝胶微孔阵列可以在单次移液步骤中实现高通量平行形成球体，并为下游应用轻松检索。 动态培养条件刺激内皮细胞和间充质干细胞共培养，以自组织形成球状体内的血管结构。 将血管化球体注射入小鼠，并观察4-7天内植入血管的快速灌注。

    TISSUE-ENGINEERED ENDOTHELIAL AND EPITHELIAL IMPLANTS DIFFERENTIALLY AND SYNERGISTICALLY REGULATE TISSUE REPAIR
    8.
    发明申请
    TISSUE-ENGINEERED ENDOTHELIAL AND EPITHELIAL IMPLANTS DIFFERENTIALLY AND SYNERGISTICALLY REGULATE TISSUE REPAIR 审中-公开
    组织工程内皮和上皮植入物不同程度地和协调地调节组织修复

    公开(公告)号：WO2009061334A1

    公开(公告)日：2009-05-14

    申请号：PCT/US2008/005922

    申请日：2008-05-06

    Applicant: MASSACHUSETTS INSTITUTE OF TECHNOLOGY ,  EDELMAN, Elazer, R. ,  ZANI, Brett

    Inventor： EDELMAN, Elazer, R. ,  ZANI, Brett

    IPC: A61K35/42 ,  A61L27/38 ,  C12N5/06

    CPC classification number: A61K35/36 ,  A61K35/12 ,  A61K35/42 ,  A61L27/3808 ,  C12N5/0688 ,  C12N2500/38 ,  C12N2501/11 ,  C12N2501/115 ,  C12N2501/25 ,  C12N2501/385 ,  C12N2501/39 ,  C12N2501/395 ,  C12N2501/81 ,  C12N2502/28 ,  C12N2533/00 ,  C12N2533/54

    Abstract: Endothelial implants restore vascular homeostasis after injury without reconstituting vascular architecture. Endothelial cells line the vascular epithelium and underlying vasa vasorum precluding distinction between cellular controls. Unlike blood vessels, the airway epithelium is highly differentiated and distinct from endothelial cells that line the bronchial vasa allowing investigation of the differential control tissue engineered cells may provide in airways and blood vessels. Through airway injury and cell culture models, tissue engineered implants of the bronchial epithelium and endothelium were found to promote synergistic repair of the airway through biochemical regulation of the airway microenvironment. While epithelial cells modulate local tissue composition and reaction, endothelial cells preserve the epithelium; together their relative impact was enhanced suggesting both cell types act synergistically for airway repair.

    Abstract translation: 内皮植入物在损伤后恢复血管稳态，而不会重建血管结构。 内皮细胞对血管上皮细胞和下面的vasa血管排除细胞对照之间的区别。 与血管不同，气道上皮与血管内皮细胞高度分化，区别于支气管血管，允许调查差异对照组织工程细胞可能在气道和血管中提供。 通过气道损伤和细胞培养模型，发现支气管上皮和内皮的组织工程植入物通过气道微环境的生物化学调节促进气道的协同修复。 虽然上皮细胞调节局部组织组成和反应，内皮细胞保留上皮细胞; 一起，他们的相对影响被增强，表明两种细胞类型协同作用用于气道修复。

    COMPOSITIONS, SOLUTIONS, AND METHODS USED FOR TRANSPLANTATION
    9.
    发明申请
    COMPOSITIONS, SOLUTIONS, AND METHODS USED FOR TRANSPLANTATION 审中-公开
    组合物，解决方案和用于运输的方法

    公开(公告)号：WO2004034887A2

    公开(公告)日：2004-04-29

    申请号：PCT/US2003/033068

    申请日：2003-10-17

    Applicant: THE GENERAL HOSPITAL CORPORATION ,  BERTHIAUME, François ,  YARMUSH, Martin ,  MOKUNO, Yasuji

    Inventor： BERTHIAUME, François ,  YARMUSH, Martin ,  MOKUNO, Yasuji

    IPC: A61B

    CPC classification number: C12N5/067 ,  A01N1/02 ,  A01N1/0226 ,  C12N2501/33 ,  C12N2501/335 ,  C12N2501/39 ,  C12N2501/81

    Abstract: This invention discloses a method for reducing the intracellular lipid storage material of a cell, tissue, or organ for transplantation and features solutions, methods and kits that induce the metabolic elimination of lipid storage in a cell, tissue, or organ. In one exemplary approach, the process involves contacting a cell, tissue, or organ with a perfusate solution that include catabolic hormones and amino acids, at physiological conditions, to increase lipid export and lipid oxidation. If desired, the cell, tissue, or organ of the invention may also be heat shock preconditioned. The invention can be used to prepare, recondition, or store a cell, tissue, or organ for transplantation by increasing tolerance to ischemia-reperfusion and cold-preservation related injury.

    Abstract translation: 本发明公开了一种减少用于移植的细胞，组织或器官的细胞内脂质储存材料的方法，其特征在于诱导脂质储存在细胞，组织或器官中的代谢消除的溶液，方法和试剂盒。 在一个示例性方法中，该方法包括在生理条件下使细胞，组织或器官与包括分解代谢激素和氨基酸的灌注液接触，以增加脂质输出和脂质氧化。 如果需要，本发明的细胞，组织或器官也可以是热休克预处理的。 本发明可用于通过增加对缺血再灌注和冷保存相关损伤的耐受性来制备，修复或储存用于移植的细胞，组织或器官。

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