公开(公告)号：WO2018053350A9

公开(公告)日：2018-03-22

申请号：PCT/US2017/051909

申请日：2017-09-15

Applicant: LABCYTE INC.

Inventor： HARDEE, Jennifer M. ,  ELLSON, Richard N. ,  STEARNS, Richard G. ,  HADIMIOGLU, Babur ,  OLECHNO, Joseph D. ,  BLAUWKAMP, Marsha N.

IPC: C12N15/87

Abstract: A method is provided for achieving transfection of host cells using sonoporation. An acoustic radiation generator is positioned in acoustic coupling relationship with respect to a reservoir containing host cells to be transfected, exogenous material to be incorporated into the host cells, and a cell-compatible fluid medium. The acoustic radiation generator is activated to generate acoustic radiation and direct the acoustic radiation into the reservoir in a manner effective to enable transfection of the host cells with the exogenous material.

公开(公告)号：WO2019191587A3

公开(公告)日：2019-10-03

申请号：PCT/US2019/024837

申请日：2019-03-29

Applicant: LABCYTE INC.

Inventor： HADIMIOGLU, Babur ,  DAULTON, Jay ,  ELLSON, Richard N.

IPC: B06B1/06 ,  G10K11/30 ,  G10K11/02

Abstract: A fluid impermeable transducer includes an assembly of a transducer head and a casing, and an actuator disposed in the casing rearward of the back of the transducer head and operable to transmit acoustic energy through the transducer head. The transducer head and casing define a working portion of the transducer that is fluid impermeable.

公开(公告)号：WO2019191587A2

公开(公告)日：2019-10-03

申请号：PCT/US2019/024837

申请日：2019-03-29

Applicant: LABCYTE INC.

Inventor： HADIMIOGLU, Babur ,  DAULTON, Jay ,  ELLSON, Richard N.

IPC: B06B1/06 ,  G10K11/30 ,  G10K11/02

Abstract: A fluid impermeable transducer includes an assembly of a transducer head and a casing, and an actuator disposed in the casing rearward of the back of the transducer head and operable to transmit acoustic energy through the transducer head. The transducer head and casing define a working portion of the transducer that is fluid impermeable.

公开(公告)号：WO2018053350A1

公开(公告)日：2018-03-22

申请号：PCT/US2017/051909

申请日：2017-09-15

Applicant: LABCYTE INC.

Inventor： HARDEE, Jennifer M. ,  ELLSON, Richard N. ,  STEARNS, Richard G. ,  HADIMIOGLU, Babur ,  OLECHNO, Joseph D. ,  BLAUWKAMP, Marsha N.

IPC: C12N15/87

Abstract: A method is provided for achieving transfection of host cells using sonoporation. An acoustic radiation generator is positioned in acoustic coupling relationship with respect to a reservoir containing host cells to be transfected, exogenous material to be incorporated into the host cells, and a cell-compatible fluid medium. The acoustic radiation generator is activated to generate acoustic radiation and direct the acoustic radiation into the reservoir in a manner effective to enable transfection of the host cells with the exogenous material.

Abstract translation: 提供了使用声强实现宿主细胞转染的方法。 声辐射发生器相对于含有待转染的宿主细胞，待并入宿主细胞中的外源性物质和细胞相容性流体介质的储库声学耦合关系定位。 激活声辐射发生器以产生声辐射，并以有效使得能够用外源材料转染宿主细胞的方式将声辐射引导到贮存器中。