公开(公告)号：WO2005089102A9

公开(公告)日：2006-03-16

申请号：PCT/US2005003856

申请日：2005-02-04

Applicant: NEOSE TECHNOLOGIES INC ,  SARIBAS SAMI ,  HAKES DAVID ,  WILLETT SCOTT ,  JOHNSON KARL F ,  BEZILA DANIEL JAMES ,  DEFREES SHAWN

Inventor： SARIBAS SAMI ,  HAKES DAVID ,  WILLETT SCOTT ,  JOHNSON KARL F ,  BEZILA DANIEL JAMES ,  DEFREES SHAWN

IPC: C12N9/10

CPC classification number: C12N9/1081 ,  C12N9/1048

Abstract: The present invention provides methods of refolding mammalian glycosyltransferases that have been produced in bacterial cells, and methods to use such refolded glycosyltransferases, including glycosyltransferase mutants that have enhanced ability to be refolded. The invention also provides methods of refolding more than one glycosyltransferase in a single vessel, methods to use such refolded glycosyltransferases, and reaction mixtures comprising the refolded glycosyltransferases.

Abstract translation: 本发明提供了在细菌细胞中产生的哺乳动物糖基转移酶重折叠的方法，以及使用这种重折叠的糖基转移酶（包括具有增强的重折叠能力的糖基转移酶突变体）的方法。 本发明还提供了在单个容器中重折叠多于一种糖基转移酶的方法，使用这种重折叠的糖基转移酶的方法和包含重折叠的糖基转移酶的反应混合物。

公开(公告)号：WO2005067601A3

公开(公告)日：2006-01-19

申请号：PCT/US2005000302

申请日：2005-01-06

Applicant: NEOSE TECHNOLOGIES INC ,  JOHNSON KARL F ,  BEZILA DAN ,  NGO WINNIE ,  HAKES DAVID

Inventor： JOHNSON KARL F ,  BEZILA DAN ,  NGO WINNIE ,  HAKES DAVID

IPC: C12N15/70 ,  C12P21/06 ,  A01N43/04 ,  A01N63/00 ,  C12N5/00 ,  C12N15/00 ,  C12N15/63

CPC classification number: C12N15/70 ,  A61K38/45 ,  A61K2039/53 ,  C12Y204/99004 ,  C12Y207/07043

Abstract: The present invention relates to methods of providing a protein product to a customer. In particular, the invention relates methods of using protein expression vectors to produce proteins to be provided to a client. The invention also provides vectors for the cloning and expression of proteins, including reagent proteins and therapeutic proteins.

Abstract translation: 本发明涉及向顾客提供蛋白质产品的方法。 特别地，本发明涉及使用蛋白质表达载体产生待提供给客户的蛋白质的方法。 本发明还提供用于克隆和表达蛋白质的载体，包括试剂蛋白质和治疗性蛋白质。

公开(公告)号：WO2005118798A2

公开(公告)日：2005-12-15

申请号：PCT/US2005/019451

申请日：2005-06-01

Applicant: NEOSE TECHNOLOGIES, INC. ,  THE UNIVERSITY OF BRITISH COLUMBIA UNIVERSITY-INDUSTRY LIAISON OFFICE ,  JOHNSON, Karl, F. ,  DEFREES, Shawn ,  WITHERS, Stephen ,  VAUGHAN, Mark

Inventor： JOHNSON, Karl, F. ,  DEFREES, Shawn ,  WITHERS, Stephen ,  VAUGHAN, Mark

IPC: C12N9/24

CPC classification number: C12P19/26 ,  C12N9/2402 ,  C12Y302/01123

Abstract: The present invention relates to a novel endoglycoceramidase whose hydrolytic activity has been substantially reduced or eliminated, such that the enzyme is useful for synthesis of glycolipids from a monosaccharide or oligosaccharide and a ceramide. More specifically, the endoglycoceramidase is a mutant version of a naturally occurring endoglycoceramidase, preferably comprising a mutation within the active site or the nucleophilic site of the enzyme and more preferably comprising a substitution mutation of the Glu residue within the active site or the nucleophilic site. Also disclosed are a method for generating the mutant endoglycoceramidase and a method for enzymatically synthesizing glycolipids using this mutant enzyme.

Abstract translation: 本发明涉及其水解活性已经显着降低或消除的新型神经酰胺糖内切酶，使得该酶可用于从单糖或寡糖和神经酰胺合成糖脂。 更具体地，神经酰胺糖内切酶是天然存在的神经酰胺糖内切酶的突变形式，优选地包含酶的活性位点或亲核位点内的突变，更优选地包含活性位点或亲核位点内的Glu残基的取代突变。 还公开了产生突变型神经酰胺糖内切酶的方法和使用该突变酶酶促合成糖脂的方法。