公开(公告)号：WO2006060246A3

公开(公告)日：2007-04-05

申请号：PCT/US2005042385

申请日：2005-11-21

申请人： DHARMACON INC ,  ROBERTSON BARBARA ,  LEAKE DEVIN ,  ROBINSON KATHRYN ,  MARSHALL WILLIAM S ,  KHVOROVA ANASTASIA

发明人： ROBERTSON BARBARA ,  LEAKE DEVIN ,  ROBINSON KATHRYN ,  MARSHALL WILLIAM S ,  KHVOROVA ANASTASIA

IPC分类号： C07H19/00 ,  A61K31/70 ,  C12N15/87

CPC分类号： C12N15/87 ,  C12N15/111 ,  C12N2310/14 ,  C12N2320/11 ,  C12N2330/30

摘要： An RTF testing plate can include at least a first control well including a substantially dry first control composition having at least a first control siRNA. The first control siRNA is capable of providing a first indication of the gene silencing efficacy. Additionally, the first control composition can be configured such that the first control siRNA is capable of being solubilized or suspended in an aqueous medium in an amount sufficient for transfecting cells in the first control well. The control siRNA can be any one of a transfection control siRNA, positive control siRNA, or negative control siRNA. Optionally, the total amount of control siRNA in the first control composition can be present in an amount for transfecting cells in only the first control well.

摘要翻译： RTF测试板可以包括至少第一对照孔，其包含具有至少第一对照siRNA的基本上干的第一对照组合物。 第一对照siRNA能够提供基因沉默功效的第一指示。 另外，第一对照组合物可以配置成使得第一对照siRNA能够以足以转染第一对照孔中的细胞的量溶解或悬浮在水性介质中。 对照siRNA可以是转染对照siRNA，阳性对照siRNA或阴性对照siRNA中的任一种。 任选地，第一对照组合物中对照siRNA的总量可以以仅在第一对照孔中转染细胞的量存在。

公开(公告)号：WO2006071410A2

公开(公告)日：2006-07-06

申请号：PCT/US2005/042404

申请日：2005-11-21

申请人： DHARMACON, INC. ,  ROBERTSON, Barbara ,  LEAKE, Devin ,  ROBINSON, Kathryn ,  MARSHALL, William, S. ,  KHVOROVA, Anastasia

发明人： ROBERTSON, Barbara ,  LEAKE, Devin ,  ROBINSON, Kathryn ,  MARSHALL, William, S. ,  KHVOROVA, Anastasia

IPC分类号： C12Q1/68 ,  C12N15/87 ,  A61K48/00

CPC分类号： C12N15/111 ,  C12N2310/14 ,  C12N2320/12 ,  C12N2330/31

摘要： A reverse transfection apparatus can be used for introducing siRNA into a cell to effect gene silencing. Such an apparatus can include a well plate having a well configured for transfecting cells. The well can include a substantially dry gene silencing composition that has at least a first siRNA which silences a first target gene. The gene silencing composition can be configured such that the at least first siRNA is capable of being solubilized or suspended in an aqueous medium in an amount sufficient for transfecting cells in the well. Additionally, the at least first siRNA can include a modification or a conjugate. The reverse transfection apparatus can be provided as a kit or system that additionally includes cells, polynucleotide carriers, reverse transfection reagents, and the like.

摘要翻译： 反向转染装置可用于将siRNA引入细胞以进行基因沉默。 这样的装置可以包括具有良好配置用于转染细胞的孔板。 该孔可以包括至少具有使第一靶基因沉默的第一siRNA的基本上干的基因沉默组合物。 基因沉默组合物可以配置成使得至少第一siRNA能够以足以转染孔中的细胞的量溶解或悬浮在水性介质中。 另外，至少第一siRNA可以包括修饰或缀合物。 反向转染装置可以作为另外包含细胞，多核苷酸载体，反向转染试剂等的试剂盒或系统来提供。