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    METHODS AND COMPOSITIONS FOR THE SPECIFIC INHIBITION OF GENE EXPRESSION BY DOUBLE-STRANDED RNA
    1.
    发明申请
    METHODS AND COMPOSITIONS FOR THE SPECIFIC INHIBITION OF GENE EXPRESSION BY DOUBLE-STRANDED RNA 审中-公开
    通过双重条带RNA特异性抑制基因表达的方法和组合物

    公开(公告)号：WO2005089287A9

    公开(公告)日：2017-03-02

    申请号：PCT/US2005008477

    申请日：2005-03-15

    申请人： HOPE CITY ,  INTEGRATED DNA TECH INC ,  ROSSI JOHN J ,  BEHLKE MARK A ,  KIM DONGHO

    发明人： ROSSI JOHN J ,  BEHLKE MARK A ,  KIM DONGHO

    IPC分类号： C07H21/04 ,  A61K48/00 ,  C07H21/02 ,  C12N15/11 ,  C12N15/113 ,  C12N15/85 ,  C12Q1/68

    CPC分类号： C12N15/113 ,  C12N15/111 ,  C12N2310/14 ,  C12N2310/33 ,  C12N2310/50 ,  C12N2310/51 ,  C12N2320/30 ,  C12N2320/50 ,  C12N2320/51 ,  C12N2330/30

    摘要： The invention is directed to compositions and methods for selectively reducing the expression of a gene product from a desired target gene in a cell, as well as for treating diseases caused by the expression of the gene. More particularly, the invention is directed to compositions that contain double stranded RNA ('dsRNA'), and methods for preparing them, that are capable of reducing the expression of target genes in eukaryotic cells. The dsRNA has a first oligonucleotide sequence that is between 25 and about 30 nucleotides in length and a second oligonucleotide sequence that anneals to the first sequence under biological conditions. In addition, a region of one of the sequences of the dsRNA having a sequence length of at least 19 nucleotides is sufficiently complementary to a nucleotide sequence of the RNA produced from the target gene to trigger the destruction of the target RNA by the RNAi machinery.

    摘要翻译： 本发明涉及用于选择性地降低来自细胞中所需靶基因的基因产物的表达以及用于治疗由基因表达引起的疾病的组合物和方法。 更具体地，本发明涉及含有双链RNA（'dsRNA'）的组合物及其制备方法，其能够降低真核细胞中靶基因的表达。 dsRNA具有长度为25至约30个核苷酸的第一寡核苷酸序列和在生物条件下与第一序列退火的第二寡核苷酸序列。 此外，具有至少19个核苷酸的序列长度的dsRNA的序列之一的区域与由靶基因产生的RNA的核苷酸序列充分互补，以通过RNAi机制触发靶RNA的破坏。

    NON-ENZYMATIC, SALT-MEDIATED SYNTHESIS OF POLYNUCLEIC ACIDS
    6.
    发明申请
    NON-ENZYMATIC, SALT-MEDIATED SYNTHESIS OF POLYNUCLEIC ACIDS 审中-公开
    非酶，盐介导的多核苷酸合成

    公开(公告)号：WO2015042073A1

    公开(公告)日：2015-03-26

    申请号：PCT/US2014/055951

    申请日：2014-09-16

    申请人： THE REGENTS OF THE UNIVERSITY OF CALIFORNIA ,  UNIVERSITY PIERRE AND MARIE CURIE

    发明人： DEAMER, David, W. ,  MAUREL, Marie-Christine ,  DA SILVA, Laura

    IPC分类号： C07H19/048 ,  C07H19/06 ,  C07H21/00 ,  C12Q1/68

    CPC分类号： C07H21/02 ,  C07H1/00 ,  C07H19/10 ,  C07H19/20 ,  C12N15/113 ,  C12N2310/14 ,  C12N2330/30 ,  C12Q1/6844 ,  C12Q2523/109 ,  C12Q2525/207 ,  C12Q2527/125

    摘要： Provided herein is a method for synthesizing polynucleic acids, comprising the steps of (a) providing an acidic solution substantially free of nucleic acid polymerase and lipids, but containing mononucleotides and a monovalent salt; (b) drying and resolubilizing the mixture of step (a) a plurality of times; and (c) recovering polynucleic acids from a resolubilized mixture of step (b). In certain aspects, the method further uses a low pH, e.g. about 3; it can utilize monophosphates, such as AMP rather than ATP; and it can be used with a polynucleotide template to form a sequence at least partially complementary to said template. Thus, both single-stranded and double-stranded polynucleic acids are provided. Ammonia salts have been used to obtain RNA lengths from 10 to 300 nucleotides after 16 half hour cycles and an effective temperature includes between 80 °C and 100 °C.

    摘要翻译： 本文提供了一种合成多核酸的方法，包括以下步骤：（a）提供基本上不含核酸聚合酶和脂质但含有单核苷酸和单价盐的酸性溶液; （b）将步骤（a）的混合物多次干燥和再溶解; 和（c）从步骤（b）的再溶解混合物中回收多核酸。 在某些方面，该方法还使用低pH，例如 约3; 它可以利用单磷酸盐，如AMP而不是ATP; 并且其可与多核苷酸模板一起使用以形成与所述模板至少部分互补的序列。 因此，提供了单链和双链多核酸。 已经使用氨盐在16个半小时循环后获得10至300个核苷酸的RNA长度，并且有效温度包括在80℃和100℃之间。