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    HIGH-PURITY STEVIOL GLYCOSIDES
    53.
    发明申请
    HIGH-PURITY STEVIOL GLYCOSIDES 审中-公开

    公开(公告)号：WO2020112957A1

    公开(公告)日：2020-06-04

    申请号：PCT/US2019/063543

    申请日：2019-11-27

    申请人： PURECIRCLE USA INC.

    发明人： MARKOSYAN, Avetik ,  CHOW, Siew Yin ,  NIZAM BIN NAWI, Khairul ,  CHKHAN, Kristina ,  AFZAAL BIN HASIM, Mohamad ,  RAMANDACH, Saravanan, A/L

    IPC分类号： C07H1/00 ,  C07H15/256 ,  C12P19/56

    摘要： Methods of preparing highly purified steviol glycosides, particularly steviolmonoside, steviolmonoside A, steviolbioside, steviolbioside D, rubusoside, steviolbioside A, steviolbioside B, rebaudioside B, stevioside, rebaudioside G, stevioside A, stevioside B, stevioside C, rebaudioside A, rebaudioside E, rebaudioside E2, rebaudioside E4, rebaudioside E6, rebaudioside E3, rebaudioside D, rebaudioside 1, rebaudioside AM, rebaudioside D7, rebaudioside M, rebaudioside M4, rebaudioside 1a, rebaudioside 1b, rebaudioside 1c, rebaudioside 1d, rebaudioside 1e, rebaudioside 1f rebaudioside 1g, rebaudioside 1h, rebaudioside 1i, rebaudioside 1j, rebaudioside 1k, rebaudioside 1l, rebaudioside 1m, rebaudioside 1n, rebaudioside 2a and/or SvG7 are described. The methods include utilizing enzyme preparations and recombinant microorganisms for converting various staring compositions to target steviol glycosides. The highly purified steviol glycosides are useful as non-caloric sweetener, flavor enhancer, sweetness enhancer, and foaming suppressor in edible and chewable compositions such as any beverages, confectioneries, bakery products, cookies, and chewing gums.

    复合甜味剂及其生产方法
    54.
    发明申请
    复合甜味剂及其生产方法 审中-公开

    公开(公告)号：WO2020062437A1

    公开(公告)日：2020-04-02

    申请号：PCT/CN2018/113715

    申请日：2018-11-02

    申请人： 四川盈嘉合生科技有限公司

    发明人： 王勇 ,  吕华军 ,  孙雨伟 ,  李建华 ,  裴亮 ,  华君

    IPC分类号： A23L27/30 ,  C12P19/56

    摘要： 一种口感好的甜味剂组合物，由莱鲍迪苷D和莱鲍迪苷M组成，且按重量比，莱鲍迪苷D:莱鲍迪苷M＝1.5～9:1。该甜味剂采用全细胞催化合成，以莱鲍迪苷A为底物，以重组微生物为催化剂，在蔗糖、氯化锌和柠檬酸三钠的存在下，催化底物进行反应得到，所述重组微生物含有EUGT11编码基因和UGT76G1编码基因。

    STEVIOL GLYCOSIDE TRANSPORT
    55.
    发明申请
    STEVIOL GLYCOSIDE TRANSPORT 审中-公开

    公开(公告)号：WO2019211230A1

    公开(公告)日：2019-11-07

    申请号：PCT/EP2019/060897

    申请日：2019-04-29

    申请人： DSM IP ASSETS B.V.

    发明人： VAN LEEUWEN, Johannes, Gustaaf, Ernst ,  BOER, Viktor, Marius ,  ZWARTJENS, Priscilla ,  VAN VUGT, Jos ,  DE JONG, René, Marcel

    IPC分类号： C07K14/39 ,  C12P19/56

    摘要： The disclosure provides a recombinant cell capable of producing a steviol glycoside, wherein the cell comprises a nucleic acid coding for a variant of a parent polypeptide, wherein the variant has steviol glycoside transport mediating activity, wherein the variant comprises an amino acid sequence which, when aligned with the amino acid sequence of the parent polypeptide, comprises at least one modification of the amino acid residue corresponding to any of the amino acids in the amino acid sequence of the parent polypeptide, wherein the variant has an improved ability to produce rebaudioside M and optionally other steviol glycosides extracellularly if compared with the parent polytpeptide when measured under the same conditions.

    ENGINEERED GLYCOSYLTRANSFERASES AND STEVIOL GLYCOSIDE GLUCOSYLATION METHODS
    56.
    发明申请
    ENGINEERED GLYCOSYLTRANSFERASES AND STEVIOL GLYCOSIDE GLUCOSYLATION METHODS 审中-公开

    公开(公告)号：WO2018144679A3

    公开(公告)日：2018-08-09

    申请号：PCT/US2018/016359

    申请日：2018-02-01

    申请人： CODEXIS, INC.

    发明人： VROOM, Jonathan ,  GALANIE, Stephanie, Sue ,  DELLAS, Nikki ,  LIANG, Jack ,  LIU, Joyce ,  ENTWISTLE, David ,  MOFFETT, Courtney, Dianne

    IPC分类号： C12P19/56 ,  C12N9/10

    摘要： The present invention provides engineered glycosyltransferase (GT) enzymes, polypeptides having GT activity, and polynucleotides encoding these enzymes, as well as vectors and host cells comprising these polynucleotides and polypeptides. The present invention provides engineered sucrose synthase (SuS) enzymes, polypeptides having SuS activity, and polynucleotides encoding these enzymes, as well as vectors and host cells comprising these polynucleotides and polypeptides. The present invention also provides compositions comprising the GT enzymes and methods of using the engineered GT enzymes to make products with β-glucose linkages. The present invention further provides compositions and methods for the production of rebaudiosides (e.g., rebaudioside M, rebaudioside A, rebaudioside I, and rebaudioside D). The present invention also provides compositions comprising the SuS enzymes and methods of using them. Methods for producing GT and SuS enzymes are also provided.