公开(公告)号：WO2023069865A1

公开(公告)日：2023-04-27

申请号：PCT/US2022/078071

申请日：2022-10-13

申请人： CODEXIS, INC.

发明人： CHING, Charlene ,  FORGET, Stephanie, Marie ,  KNIGHT, Anders, Matthew ,  KRAWCZYK, Mikayla, Jianghongxia ,  MAHMOODI, Niusha ,  MAYO, Melissa, Ann ,  MILLER, Mathew, G. ,  VROOM, Jonathan

IPC分类号： C12N9/10 ,  C12P19/34

摘要： The present invention provides engineered terminal deoxynucleotidyl transferase (TdT) polypeptides useful in template-independent polynucleotide synthesis capable of adding a nucleoside triphosphate-3' - O-removable blocking group (NTP-3'-O-RBG) to the 3'-OH end of a growing oligonucleotide or polynucleotide chain in a template-independent manner, as well as compositions, methods of utilizing these engineered polypeptides, and polynucleotides encoding the engineered terminal deoxynucleotidyl transferase.

公开(公告)号：WO2022076454A1

公开(公告)日：2022-04-14

申请号：PCT/US2021/053626

申请日：2021-10-05

申请人： CODEXIS, INC.

发明人： VROOM, Jonathan ,  SIVARAMAKRISHNAN, Santhosh ,  HURTAK, Jessica, Anna

IPC分类号： C12N9/90 ,  C12N15/52 ,  C12N15/70

摘要： The present invention provides engineered phosphopentomutase (PPM) enzymes, polypeptides having PPM activity, and polynucleotides encoding these enzymes, as well as vectors and host cells comprising these polynucleotides and polypeptides. Methods for producing PPM enzymes are also provided. The present invention further provides compositions comprising the PPM enzymes and methods of using the engineered PPM enzymes. The present invention finds particular use in the production of pharmaceutical compounds.

公开(公告)号：WO2021050348A1

公开(公告)日：2021-03-18

申请号：PCT/US2020/049164

申请日：2020-09-03

申请人： CODEXIS, INC.

发明人： DUAN, Da ,  SOWELL-KANTZ, Auric, Anthony ,  PETKOVA, Aksiniya, Lyubenova ,  NAZOR, Jovana ,  SUBRAMANIAN, Nandhitha ,  ALVIZO, Oscar

IPC分类号： A61K8/66 ,  A61K38/44 ,  C12N9/04

摘要： The present invention provides engineered peroxidase enzymes, polypeptides having peroxidase activity, and polynucleotides encoding these enzymes, as well as vectors and host cells comprising these polynucleotides and polypeptides. Methods for producing peroxidase enzymes are also provided. The present invention further provides compositions comprising the peroxidase enzymes and methods of using the engineered peroxidase enzymes. The present invention finds particular use in the production of pharmaceutical and other compounds.

公开(公告)号：WO2020009884A1

公开(公告)日：2020-01-09

申请号：PCT/US2019/039401

申请日：2019-06-27

申请人： CODEXIS, INC.

发明人： YI, Xiang ,  DELLAS, Nikki ,  RIGGINS, James, Nicholas ,  CHNG, Chinping ,  AGARD, Nicholas, J. ,  VALLIEU, Kristen, Jean ,  HUISMAN, Gjalt, W.

IPC分类号： C12N15/52 ,  C12N15/74

摘要： The present invention provides engineered branched chain ketoacid decarboxylase (KdcA) polypeptides and compositions thereof, as well as polynucleotides encoding the engineered branched chain ketoacid decarboxylase (KdcA) polypeptides. In some embodiments, the engineered KdcA polypeptides are optimized to provide enhanced catalytic activity, as well as reduced sensitivity to proteolysis and increased tolerance to storage at elevated temperatures. The present invention also provides methods for the use of the compositions comprising the engineered KdcA polypeptides for therapeutic and industrial purposes.

公开(公告)号：WO2019005337A1

公开(公告)日：2019-01-03

申请号：PCT/US2018/034073

申请日：2018-05-23

申请人： CODEXIS, INC.

发明人： VOLADRI, Rama ,  MICKLITSCH, Christopher, Michael ,  ALVIZO, Oscar ,  NAZOR, Jovana ,  DUAN, Da ,  VIDUYA, Judy, Victoria Antonio ,  JENNE, Stephan ,  AN, Chihui ,  CANADA, Keith, Allen ,  DEVINE, Paul, N. ,  FARASAT, Iman ,  FRYSZKOWSKA, Anna ,  LEXA, Katrina, W. ,  ORR, Robert, Kevin

IPC分类号： C12N9/80 ,  C12N9/84 ,  C12N15/09 ,  C12N15/55

摘要： The present invention provides engineered penicillin G acylase (PGA) enzymes, polynucleotides encoding the enzymes, compositions comprising the enzymes, and methods of using the engineered PGA enzymes.

公开(公告)号：WO2017213758A1

公开(公告)日：2017-12-14

申请号：PCT/US2017/028370

申请日：2017-04-19

申请人： CODEXIS, INC.

发明人： NAZOR, Jovana ,  OSBORNE, Robert ,  LIANG, Jack ,  VROOM, Jonathan ,  ZHANG, Xiyun ,  ENTWISTLE, David ,  VOLADRI, Rama ,  GARCIA, Ravi, David ,  MOORE, Jeffrey, C. ,  GROSSER, Shane ,  KOSJEK, Birgit ,  TRUPPO, Matthew

IPC分类号： C12P17/12 ,  C12P13/24

摘要： The present invention provides engineered proline hydroxylase polypeptides for the production of hydroxylated compounds, polynucleotides encoding the engineered proline hydroxylases, host cells capable of expressing the engineered proline hydroxylases, and methods of using the engineered proline hydroxylases to prepare compounds useful in the production of active pharmaceutical agents.

摘要翻译： 本发明提供了用于生产羟基化化合物的工程化脯氨酸羟化酶多肽，编码工程化脯氨酸羟化酶的多核苷酸，能够表达工程化脯氨酸羟化酶的宿主细胞，以及使用工程化脯氨酸羟化酶制备 用于生产活性药物的化合物。

公开(公告)号：WO2016179464A1

公开(公告)日：2016-11-10

申请号：PCT/US2016/031132

申请日：2016-05-06

申请人： CODEXIS, INC.

发明人： BANERJEE, Goutami ,  YANG, Jie ,  ZHANG, Xiyun ,  MILCZEK, Erika, M. ,  MAYO, Melissa, Ann ,  JENNE, Stephan

IPC分类号： C07K14/24 ,  C12P37/02 ,  C12N15/55

CPC分类号： C12N9/84 ,  C12P21/00 ,  C12Y305/01011

摘要： The present disclosure relates to engineered penicillin G acylase (PGA) enzymes having improved properties, polynucleotides encoding such enzymes, compositions including the enzymes, and methods of using the enzymes.

摘要翻译： 本公开涉及具有改进性质的工程青霉素G酰基转移酶（PGA）酶，编码这些酶的多核苷酸，包含酶的组合物和使用酶的方法。

公开(公告)号：WO2015073555A1

公开(公告)日：2015-05-21

申请号：PCT/US2014/065259

申请日：2014-11-12

申请人： CODEXIS, INC.

发明人： AGARD, Nicholas, J. ,  ALVIZO, Oscar ,  MAYO, Melissa, Ann ,  NG MINOR, Stefanie ,  RIGGINS, James, Nicholas ,  MOORE, Jeffrey, C.

IPC分类号： C07K14/305

CPC分类号： C12N9/0028 ,  C12P13/001 ,  C12P17/10 ,  C12Y105/01028

摘要： The present application provides engineered polypeptides having imine reductase activity, polynucleotides encoding the engineered imine reductases, host cells capable of expressing the engineered imine reductases, and methods of using these engineered polypeptides with a range of ketone and amine substrate compounds to prepare secondary and tertiary amine product compounds.

摘要翻译： 本申请提供具有亚胺还原酶活性的工程化多肽，编码工程改造的亚胺还原酶的多核苷酸，能够表达工程化亚胺还原酶的宿主细胞，以及使用这些工程改造的多肽与一系列酮和胺底物化合物以制备仲胺和叔胺的方法 产品化合物。

公开(公告)号：WO2015048572A1

公开(公告)日：2015-04-02

申请号：PCT/US2014/057899

申请日：2014-09-26

申请人： CODEXIS, INC.

发明人： ZHANG, Xiyun ,  SARMIENTO, Russell Javiniar ,  BASKERVILLE, Donald Scott ,  HUISMAN, Gjait W.

IPC分类号： G06F19/00 ,  G06F19/16

CPC分类号： C12N15/1089 ,  C12N15/1058 ,  C40B30/02

摘要： Disclosed are methods for identifying bio-molecules with desired properties (or which are most suitable for a round of directed evolution) from complex bio-molecule libraries or sets of such libraries. Some embodiments of the present disclosure provide methods for virtually screening proteins for beneficial properties. Some embodiments of the present disclosure provide methods for virtually screening enzymes for desired activity and/or selectivity for catalytic reactions involving particular substrates. Some embodiments combine screening and directed evolution to design and develop proteins and enzymes having desired properties. Systems and computer program products implementing the methods are also provided.

摘要翻译： 公开了用于从复杂的生物分子文库或这样的文库集合鉴定具有所需性质（或最适合于一轮定向进化）的生物分子的方法。 本公开的一些实施方案提供了用于虚拟筛选蛋白质以获得有益特性的方法。 本公开的一些实施方案提供了用于虚拟筛选酶用于涉及特定底物的催化反应的期望活性和/或选择性的方法。 一些实施方案将筛选和定向进化相结合来设计和开发具有所需性质的蛋白质和酶。 还提供了实施方法的系统和计算机程序产品。

公开(公告)号：WO2014159850A3

公开(公告)日：2014-12-04

申请号：PCT/US2014025332

申请日：2014-03-13

申请人： CODEXIS INC

发明人： VALLE FERNANDO ,  CHOUDHARY PATRICIA ,  OSBORNE ROBERT ,  ROCHA SAUL NITSCHE ,  BHATIA MONICA ,  LAO U LOI ,  ZHU YIHUI

IPC分类号： C12N1/20 ,  C07H21/04

CPC分类号： C12N15/70 ,  C07K14/245 ,  C12N9/1051 ,  C12P21/02

摘要： The present invention provides compositions and methods comprising a low-phosphate repressible promoter. In particular, the present invention provides a low-phosphate repressible promoter from E. coli.

摘要翻译： 本发明提供了包含低磷酸盐阻抑型启动子的组合物和方法。 具体而言，本发明提供了来自大肠杆菌的低磷酸盐阻抑型启动子。