公开(公告)号：WO2011066541A2

公开(公告)日：2011-06-03

申请号：PCT/US2010/058341

申请日：2010-11-30

Applicant: TRUSTEES OF BOSTON UNIVERSITY ,  MASSACHUSETTS INSTITUTE OF TECHNOLOGY ,  COLLINS, James, J. ,  LU, Timothy Kuan-Ta

Inventor： COLLINS, James, J. ,  LU, Timothy Kuan-Ta

IPC: C12N15/09 ,  C12N15/63 ,  C12Q1/68 ,  C12Q1/02

CPC classification number: C12N15/63 ,  C12N15/635

Abstract: Described herein are novel biological circuit chemotactic converter that utilize modular components, such as genetic toggle switches and single invertase memory modules (SIMMs), for detecting and converting external inputs, such as chemoattractants, into outputs that allow for autonomous chemotaxis in cellular systems. Flexibility in these biological circuit chemotactic converter is provided by combining individual modular components, i.e., SIMMs and genetic toggle switches, together. These biological converter switches can be combined in a variety of network topologies to create network systems that regulate chemotactic responses based on the combination and nature of input signals received.

Abstract translation: 这里描述的是利用诸如遗传切换开关和单转化酶存储器模块（SIMM）之类的模块化组件的新型生物电路趋化转换器，用于检测外部输入（例如化学引诱物）并将其转换成允许 用于细胞系统中的自主趋化。 这些生物电路趋化转换器的灵活性是通过将各个模块化组件，即SIMM和遗传切换开关组合在一起提供的。 这些生物转换器开关可以组合在各种网络拓扑中，以创建基于所接收的输入信号的组合和性质来调节趋化响应的网络系统。

公开(公告)号：WO2011050272A3

公开(公告)日：2011-04-28

申请号：PCT/US2010/053757

申请日：2010-10-22

Applicant: TRUSTEES OF BOSTON UNIVERSITY ,  ALTUG, Hatice ,  YANIK, Ahmet Ali ,  ERRAMILLI, Shyamsunder ,  ADATO, Ronen ,  AKSU, Serap ,  HUANG, Min ,  ARTAR, Alp

Inventor： ALTUG, Hatice ,  YANIK, Ahmet Ali ,  ERRAMILLI, Shyamsunder ,  ADATO, Ronen ,  AKSU, Serap ,  HUANG, Min ,  ARTAR, Alp

IPC: G01J3/02 ,  G01J3/44

Abstract: The present invention generally relates to nanoantenna arrays and methods of their fabrication. In particular, one aspect relates to nanoantenna arrays comprising nanostructures of predefined shapes in predefined patterns, which results in collective excitement of surface plasmons. In some embodiments the nanoantenna arrays can be used for spectroscopy and nanospectroscopy. Another aspects of the present invention relate to a method of high-throughput fabrication of nanoantenna arrays includes fabricating a reusable nanostencil for nanostensil lithography (NSL) which provides a mask to deposit materials onto virtually any support, such as flexible and thin-film stretchable supports. The nanostencil lithography methods enable high quality, high-throughput fabrication of nanostructures on conducting, non-conducting and magnetic supports. The nanostencil can be prepared by etching nanoapertures of predefined patterns into a waffer or ceramic membrane. In some embodiments, a nanoantenna array comprises plasmonic nanostructures or non-plasmonic nanostructures.

公开(公告)号：WO2010091185A2

公开(公告)日：2010-08-12

申请号：PCT/US2010/023207

申请日：2010-02-04

Applicant: TRUSTEES OF BOSTON COLLEGE ,  KANTROWITZ, Evan R. ,  HENG, Sabrina

Inventor： KANTROWITZ, Evan R. ,  HENG, Sabrina

IPC: C07D307/91

CPC classification number: C07D307/91

Abstract: The invention is directed to a compound according to formula (I). The compounds of formula (I) include prodrugs, pharmaceutically acceptable salts, stereoisomer mixtures, and enantiomers thereof. The invention is also directed to a pharmaceutical composition comprising a compound according to formula (I) and a pharmaceutically acceptable carrier and to methods for treating diabetes by administering such a pharmaceutical composition alone or in combination with other therapeutic agents. The invention is also directed to inhibitors of fructose-1,6-bisphosphatase.

Abstract translation: 本发明涉及根据式（I）的化合物。 式（I）化合物包括前药，药学上可接受的盐，立体异构体混合物和对映异构体。 本发明还涉及包含根据式（I）的化合物和药学上可接受的载体的药物组合物，并且涉及通过单独或与其他治疗剂组合施用这种药物组合物来治疗糖尿病的方法。 本发明还涉及果糖-1,6-二磷酸酶的抑制剂。

公开(公告)号：WO2010075441A1

公开(公告)日：2010-07-01

申请号：PCT/US2009/069288

申请日：2009-12-22

Applicant: TRUSTEES OF BOSTON UNIVERSITY ,  MASSACHUSETTS INSTITUTE OF TECHNOLOGY ,  COLLINS, James, J. ,  LU, Timothy Kuan-Ta

Inventor： COLLINS, James, J. ,  LU, Timothy Kuan-Ta

IPC: C12Q1/68 ,  C12N15/63

CPC classification number: C12Q1/6897 ,  C12N15/63 ,  C12N15/635 ,  C12Q2521/507

Abstract: We have created novel engineered genetic counter designs and methods of use thereof that utilize DNA recombinases to provide modular systems, termed single invertase memory modules (SIMMs), for encoding memory in cells and cellular systems. Our designs are easily extended to compute to high numbers, by utilizing the >100 known recombinases to create subsequent modules. Flexibility in our engineered genetic counter designs is provided by daisy-chaining individual modular components, i.e., SIMMs together. These modular components of the engineered genetic counters can be combined in other network topologies to create circuits that perform, amongst other things, logic and memory. Our novel engineered genetic counter designs allow for the maintenance of memory and provide the ability to count between discrete states by expressing the recombinases between their cognate recognition sites.

Abstract translation: 我们已经创建了新颖的遗传算法设计及其使用方法，其利用DNA重组酶来提供称为单一转换酶存储器模块（SIMM）的模块化系统，用于对细胞和细胞系统中的存储器进行编码。 通过使用> 100个已知重组酶来创建后续模块，我们的设计可以轻松扩展到高数量计算。 我们设计的基因计数器设计中的灵活性是通过菊花链连接单个模块化组件（即SIMM）来提供的。 设计的遗传计数器的这些模块化组件可以组合在其他网络拓扑中，以创建执行逻辑和存储器的电路。 我们的新颖设计的基因计数器设计允许维持记忆，并提供通过在其同源识别位点之间表达重组酶来提供离散状态之间的计数能力。

公开(公告)号：WO2009137136A2

公开(公告)日：2009-11-12

申请号：PCT/US2009/034296

申请日：2009-02-17

Applicant: TRUSTEES OF BOSTON UNIVERSITY ,  MASSACHUSETTS INSTITUTE OF TECHNOLOGY ,  COLLINS, James, J. ,  LU, Timothy, Kuan-Ta

Inventor： COLLINS, James, J. ,  LU, Timothy, Kuan-Ta

IPC: C12Q1/68

CPC classification number: C12N15/1086 ,  C12N15/111 ,  C12N15/63 ,  C12N2310/111 ,  C12N2320/50

Abstract: Described are methods and compositions for the detection of target genes. The inventors have developed a synthetic nucleic acid sensor-effector gene circuit. In cells without a target gene, the circuit suppresses e.g., effector production, but in the presence of the target gene the suppression is subject to competition, such that the synthetic sensor is de-repressed and permits expression of the effector gene. The methods and compositions described further permit the selective expression of an effector gene in those cells expressing the target gene. In this manner, cells expressing a target gene can be selectively targeted for treatment or elimination. In certain aspects, the methods and compositions described permit the selective expression of an agent such as a therapeutic gene product, in a specifically targeted population of cells in an organism.

Abstract translation: 描述了用于检测靶基因的方法和组合物。 本发明人开发了合成核酸传感器 - 效应子基因电路。 在没有靶基因的细胞中，电路抑制例如效应子产生，但是在靶基因的存在下，抑制受到竞争，使得合成传感器被去抑制并且允许效应基因的表达。 所描述的方法和组合物进一步允许在表达靶基因的那些细胞中选择性表达效应基因。 以这种方式，表达靶基因的细胞可以选择性地靶向用于治疗或消除。 在某些方面，所描述的方法和组合允许在生物体中的特定靶细胞群中选择性地表达诸如治疗性基因产物的试剂。

公开(公告)号：WO2009108406A3

公开(公告)日：2009-09-03

申请号：PCT/US2009/030755

申请日：2009-01-12

Applicant: TRUSTEES OF BOSTON UNIVERSITY ,  MASSACHUSETTS INSTITUTE OF TECHNOLOGY ,  COLLINS, James, J. ,  LU, Timothy, Kuan-Ta

Inventor： COLLINS, James, J. ,  LU, Timothy, Kuan-Ta

IPC: C07K14/01 ,  A61K35/76

Abstract: The present invention relates to the treatment and prevention of bacteria and bacterial infections. In particular, the present invention relates to engineered bacteriophages used in combination with antimicrobial agents to potentiate the antimicrobial effect and bacterial killing by the antimicrobial agent. The present invention generally relates to methods and compositions comprising engineered bacteriophages and antimicrobial agents for the treatment of bacteria, and more particularly to bacteriophages comprising agents that inhibit antibiotic resistance genes and/or cell survival genes, and/or bacteriophages comprising repressors of SOS response genes or inhibitors of antimicrobial defense genes and/or expressing an agent which increases the sensitivity of bacteria to an antimicrobial agent in combination with at least one antimicrobial agent, and their use thereof.

公开(公告)号：WO2007051002A2

公开(公告)日：2007-05-03

申请号：PCT/US2006/042299

申请日：2006-10-27

Applicant: THE TRUSTEES OF BOSTON UNIVERSITY ,  BROUDE, Natalia ,  CANTOR, Charles, R. ,  DEMIDOV, Vadim, V.

Inventor： BROUDE, Natalia ,  CANTOR, Charles, R. ,  DEMIDOV, Vadim, V.

IPC: C12Q1/68

CPC classification number: C12Q1/6883

Abstract: The present invention relates to a method to produce activated split-polypeptide fragments that on reconstitution immediately forms an active protein. The method relate to real-time protein complementation. Also encompassed in the invention is a method to split and produce split-fluorescent proteins in an active state which produce a fluorescent signal immediately on reconstitution. The present application also provides methods to detect nucleic acids; non-nucleic acid analytes and nucleic acid hybridization in real-time using the novel activated split-polypeptide fragments of the invention.

Abstract translation: 本发明涉及一种产生活化的裂多肽片段的方法，其在重构时立即形成活性蛋白质。 该方法涉及实时蛋白质互补。 本发明还包括分裂和产生活性状态的分裂荧光蛋白的方法，其在重建时立即产生荧光信号。 本申请还提供了检测核酸的方法; 非核酸分析物和核酸杂交，使用本发明的新型活化裂解多肽片段。

公开(公告)号：WO2007011896A2

公开(公告)日：2007-01-25

申请号：PCT/US2006/027746

申请日：2006-07-18

Applicant: THE TRUSTEES OF BOSTON UNIVERSITY ,  KIRBER, Michael, T.

Inventor： KIRBER, Michael, T.

IPC: A61M1/14 ,  A61K39/395

CPC classification number: A61M1/3472 ,  A61M1/3486 ,  A61M1/3679 ,  C07K16/22

Abstract: The present invention provides a method to reduce the amount of undesired growth factors in the circulating blood of a subject to prevent tumor growth and proliferation during or after a wound healing and/or other local tissue repair process on a subject, comprising extracorporeal adsorption of growth factors from blood of the subject and return of the treated blood to the subject.

Abstract translation: 本发明提供一种减少受试者循环血液中不期望的生长因子的量以防止在受试者的伤口愈合和/或其他局部组织修复过程中或之后的肿瘤生长和增殖的方法，其包括体外吸附生长 受试者的血液和受治疗的血液返回受试者的因素。

公开(公告)号：WO2006137847A3

公开(公告)日：2006-12-28

申请号：PCT/US2005/032365

申请日：2005-09-12

Applicant: TRUSTEES OF BOSTON UNIVERSITY ,  MASSACHUSETTS INSTITUTE OF TECHNOLOGY ,  COLLINS, James, J. ,  KOBAYASHI, Hideki ,  KEARN, Mads ,  ARAKI, Michihiro ,  FRIEDLAND, Ari ,  KUAN-TA LU, Timothy

Inventor： COLLINS, James, J. ,  KOBAYASHI, Hideki ,  KEARN, Mads ,  ARAKI, Michihiro ,  FRIEDLAND, Ari ,  KUAN-TA LU, Timothy

IPC: C12N15/00

Abstract: The present invention provides engineered bacteriophages that express at least one biofilm degrading enzyme on their surface and uses thereof for degrading bacterial biofilms. The invention also provides genetically engineered bacteriophages expressing the biofilm degrading enzymes and proteins necessary for the phage to replicate in different naturally occurring biofilm producing bacteria. The phages of the invention allow a method of biofilm degradation by the use of one or only a few administration of the phage because the system using these phages is self perpetuating, and capable of degrading biofilm even when the concentration of bacteria within the biofilm is low.

公开(公告)号：WO2005047451A3

公开(公告)日：2005-05-26

申请号：PCT/US2004/037764

申请日：2004-11-12

Applicant: TRUSTEES OF BOSTON UNIVERSITY ,  BRODY, Jerome, S. ,  SPIRA, Avrum

Inventor： BRODY, Jerome, S. ,  SPIRA, Avrum

IPC: C12Q1/68 ,  C12P19/34 ,  C07H21/04

Abstract: The present invention is directed to a scraping instrument for collection of a biological sample, and a non-invasive method for obtaining nucleic acid from buccal mucosa epithelial cells using the scraping instrument. Such nucleic acid can be used for example for gene expression profiling, including to assess lung disease risk associated with airway pollutants.