公开(公告)号：WO2003076463A3

公开(公告)日：2003-09-18

申请号：PCT/NL2003/000178

申请日：2003-03-11

申请人： WAGENINGEN CENTRE FOR FOOD SCIENCES ,  KARATZAS, Kimon, Andreas ,  BENNIK, Maria, Henrica, Jacoba ,  ABEE, Tjakko ,  KLEEREBEZEM, Michiel ,  DE VOS, Willem, Meindert

发明人： KARATZAS, Kimon, Andreas ,  BENNIK, Maria, Henrica, Jacoba ,  ABEE, Tjakko ,  KLEEREBEZEM, Michiel ,  DE VOS, Willem, Meindert

IPC分类号： C12N15/31

摘要： The present invention relates to Gram positive bacteria with increased stress tolerance, reduced virulence, and/or reduced mobility, as a result of an altered CtsR protein. The alteration of the CtsR protein in particular concerns an alteration of the conserved glycine-rich region in the CtsR protein. The invention further relates to nucleic acid encoding the altered CtsR proteins. The altered Gram positive bacteria may be used in fermentation processes and may be used as delivery vehicles for site specific delivery of therapeutic proteins such as vaccines.

公开(公告)号：WO03076463A8

公开(公告)日：2004-10-07

申请号：PCT/NL0300178

申请日：2003-03-11

申请人： WAGENINGEN CT FOR FOOD SCIENCE ,  KARATZAS KIMON ANDREAS ,  BENNIK MARIA HENRICA JACOBA ,  ABEE TJAKKO ,  KLEEREBEZEM MICHIEL ,  DE VOS WILLEM MEINDERT

发明人： KARATZAS KIMON ANDREAS ,  BENNIK MARIA HENRICA JACOBA ,  ABEE TJAKKO ,  KLEEREBEZEM MICHIEL ,  DE VOS WILLEM MEINDERT

IPC分类号： C07K14/195 ,  C07K14/32 ,  C07K14/335 ,  C12N1/21 ,  C12N15/31 ,  A61K35/74 ,  A61K39/02 ,  C12P1/04

CPC分类号： A61K35/747 ,  A61K2039/522 ,  C07K14/195 ,  C07K14/32 ,  C07K14/335

摘要： The present invention relates to Gram positive bacteria with increased stress tolerance, reduced virulence, and/or reduced mobility, as a result of an altered CtsR protein. The alteration of the CtsR protein in particular concerns an alteration of the conserved glycine-rich region in the CtsR protein. The invention further relates to nucleic acid encoding the altered CtsR proteins. The altered Gram positive bacteria may be used in fermentation processes and may be used as delivery vehicles for site specific delivery of therapeutic proteins such as vaccines.

摘要翻译： 本发明涉及由于改变的CtsR蛋白而具有增加的胁迫耐受性，降低的毒力和/或降低的迁移率的革兰氏阳性细菌。 CtsR蛋白的改变特别涉及CtsR蛋白中保守的富含甘氨酸的区域的改变。 本发明还涉及编码改变的CtsR蛋白的核酸。 改变的革兰氏阳性细菌可用于发酵过程中，并且可以用作用于特异性递送治疗性蛋白质例如疫苗的递送载体。

公开(公告)号：WO2003076463A2

公开(公告)日：2003-09-18

申请号：PCT/NL2003/000178

申请日：2003-03-11

申请人： WAGENINGEN CENTRE FOR FOOD SCIENCES ,  KARATZAS, Kimon, Andreas ,  BENNIK, Maria, Henrica, Jacoba ,  ABEE, Tjakko ,  KLEEREBEZEM, Michiel ,  DE VOS, Willem, Meindert

发明人： KARATZAS, Kimon, Andreas ,  BENNIK, Maria, Henrica, Jacoba ,  ABEE, Tjakko ,  KLEEREBEZEM, Michiel ,  DE VOS, Willem, Meindert

IPC分类号： C07K14/195

CPC分类号： A61K35/747 ,  A61K2039/522 ,  C07K14/195 ,  C07K14/32 ,  C07K14/335

摘要： The present invention relates to Gram positive bacteria with increased stress tolerance, reduced virulence, and/or reduced mobility, as a result of an altered CtsR protein. The alteration of the CtsR protein in particular concerns an alteration of the conserved glycine-rich region in the CtsR protein. The invention further relates to nucleic acid encoding the altered CtsR proteins. The altered Gram positive bacteria may be used in fermentation processes and may be used as delivery vehicles for site specific delivery of therapeutic proteins such as vaccines.

摘要翻译： 本发明涉及由于改变的CtsR蛋白而具有增加的胁迫耐受性，降低的毒力和/或降低的迁移率的革兰氏阳性细菌。 CtsR蛋白的改变特别涉及CtsR蛋白中保守的富含甘氨酸的区域的改变。 本发明还涉及编码改变的CtsR蛋白的核酸。 改变的革兰氏阳性细菌可用于发酵过程中，并且可以用作用于特异性递送治疗性蛋白质例如疫苗的递送载体。