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    METHODS FOR SEQUENTIAL REPLACEMENT OF TARGETED REGION BY HOMOLOGOUS RECOMBINATION
    1.
    发明申请
    METHODS FOR SEQUENTIAL REPLACEMENT OF TARGETED REGION BY HOMOLOGOUS RECOMBINATION 审中-公开
    通过同源重建方法顺序替代靶向区域的方法

    公开(公告)号：WO2009076464A3

    公开(公告)日：2009-09-24

    申请号：PCT/US2008086275

    申请日：2008-12-10

    Applicant: ALIVA BIOPHARMACEUTICALS INC ,  SHIZUYA HIROAKI ,  SINGH MANISH ,  ROH CECILIA

    Inventor： SHIZUYA HIROAKI ,  SINGH MANISH ,  ROH CECILIA

    IPC: C12N15/09 ,  C12N15/31 ,  C12N15/65

    CPC classification number: C12N15/64 ,  C12N15/10 ,  C12N15/902

    Abstract: The invention provides methods and compositions for generating non-human transgenic cells and organisms that are transgenic at one or more gene sequences by separately recombining fragments of a complete gene in temporal sequence. According to the methods of the invention, a set of DNA constructs containing a non-endogenous DNA sequence flanked and/or operably linked at its ends by sequences from the non-human organism are generated by recombination in a bacterial cell, for example, in E. coli. The DNA constructs that are produced can then be introduced into a non-human homologous recombination competent cell where successive cells will contain recombined segments of a target gene, with the ultimate cell in a line containing an endogenous target gene completely replaced by genomic DNA of another species.

    Abstract translation: 本发明提供用于产生通过在时间顺序上单独重组完整基因的片段而在一个或多个基因序列上转基因的非人转基因细胞和生物体的方法和组合物。 根据本发明的方法，通过在细菌细胞中的重组产生一组含有来自非人生物体的序列在其末端侧翼和/或可操作地连接的非内源DNA序列的DNA构建体，例如在 大肠杆菌 然后可以将所产生的DNA构建体导入非人类同源重组感受态细胞，其中连续细胞将包含靶基因的重组区段，最终细胞在含有内源性靶基因的系中完全被其他基因组DNA替代 种类。

    METHODS FOR SEQUENTIAL REPLACEMENT OF TARGETED REGION BY HOMOLOGOUS RECOMBINATION
    2.
    发明申请
    METHODS FOR SEQUENTIAL REPLACEMENT OF TARGETED REGION BY HOMOLOGOUS RECOMBINATION 审中-公开
    通过同源重组序列替换靶区的方法

    公开(公告)号：WO2009076464A2

    公开(公告)日：2009-06-18

    申请号：PCT/US2008/086275

    申请日：2008-12-10

    Applicant: ALIVA BIOPHARMACEUTICALS, INC. ,  SHIZUYA, Hiroaki ,  SINGH, Manish ,  ROH, Cecilia

    Inventor： SHIZUYA, Hiroaki ,  SINGH, Manish ,  ROH, Cecilia

    IPC: C12N15/09 ,  C12N15/31 ,  C12N15/65

    CPC classification number: C12N15/64 ,  C12N15/10 ,  C12N15/902

    Abstract: The invention provides methods and compositions for generating non-human transgenic cells and organisms that are transgenic at one or more gene sequences by separately recombining fragments of a complete gene in temporal sequence. According to the methods of the invention, a set of DNA constructs containing a non-endogenous DNA sequence flanked and/or operably linked at its ends by sequences from the non-human organism are generated by recombination in a bacterial cell, for example, in E. coli. The DNA constructs that are produced can then be introduced into a non-human homologous recombination competent cell where successive cells will contain recombined segments of a target gene, with the ultimate cell in a line containing an endogenous target gene completely replaced by genomic DNA of another species.

    Abstract translation: 本发明提供了通过在时间序列中分开重组完整基因的片段来产生在一个或多个基因序列处转基因的非人转基因细胞和生物体的方法和组合物。 根据本发明的方法，通过在细菌细胞中重组产生含有在其两端侧翼和/或可操作地连接来自非人生物的非内源DNA序列的DNA构建体，例如在 电子。 然后可以将所产生的DNA构建体导入非人同源重组感受态细胞中，其中连续的细胞将含有靶基因的重组片段，最终细胞在含有完全内源靶基因的系中 被另一物种的基因组DNA替换。

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