公开(公告)号：WO2021206614A1

公开(公告)日：2021-10-14

申请号：PCT/SE2021/050306

申请日：2021-04-06

Applicant: APLEX BIO AB

Inventor： NASEEM, Umear ,  SOARES, Ruben Rafael Gonçalves

IPC: G01N33/543 ,  G01N33/58

Abstract: The disclosure relates to a method for multiplexed detection of a plurality of target biomolecules using optical encoding, wherein each target biomolecule has at least one detection target, comprising the steps of: a. providing one or more nanoparticle types comprising a plurality of nanoparticles, each nanoparticle having a coating that provides binding affinity of the nanoparticle to a type-specific detection target, and wherein each nanoparticle comprises a plurality of fluorophores that generates a signal which is unique for each nanoparticle type; b. providing a sample comprising a plurality of target biomolecules; c. contacting the sample with the plurality of nanoparticle types, thereby allowing the nanoparticles to bind with the detection targets of the target biomolecules; d. optically decoding the fluorophore signals emitted by the nanoparticle of the nanoparticle type bound to the detection target of the target biomolecules by measuring the wavelength and intensity of the emitted signals, thereby detecting the presence and identity of the target biomolecules.

公开(公告)号：WO2023057582A1

公开(公告)日：2023-04-13

申请号：PCT/EP2022/077835

申请日：2022-10-06

Applicant: APLEX BIO AB

Inventor： NASEEM, Umear ,  KUNZE, Klaus

IPC: B01J13/02 ,  B01J13/20 ,  B22F1/054 ,  B22F1/102 ,  B22F1/16 ,  C01B33/113 ,  C01B33/18 ,  C01B33/26 ,  C09B67/08 ,  B01J13/14 ,  B01J13/22 ,  C09B67/0005 ,  C09B67/0033 ,  C09B67/0097

Abstract: The present disclosure relates to a nanoparticle which comprises a core comprising a first fluorophore, preferably a semiconductor, and a first layer comprising a second fluorophore, wherein the emission and/or excitation wavelength of the first fluorophore is different to the emission and/or excitation wavelength of the second fluorophore, along with processes for preparing such a nanoparticle, methods for the detection of target biomolecules using such a nanoparticle, uses of such a nanoparticle and a kit-of-parts comprising such nanoparticle.