公开(公告)号：WO2010102172A1

公开(公告)日：2010-09-10

申请号：PCT/US2010/026308

申请日：2010-03-05

Applicant: ATHENIX CORPORATION ,  SAMPSON, Kimberly, S. ,  TOMSO, Daniel, J.

Inventor： SAMPSON, Kimberly, S. ,  TOMSO, Daniel, J.

IPC: C12N15/82 ,  C07K14/325

CPC classification number: C07K14/325 ,  A01N37/46 ,  C07K14/32 ,  C12N15/8286 ,  Y02A40/162

Abstract: Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a delta-endotoxin polypeptide are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated delta-endotoxin nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed, and antibodies specifically binding to those amino acid sequences. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO: 13-24, or the nucleotide sequence set forth in SEQ ID NO: 1-12 and 25-44, as well as variants and fragments thereof.

Abstract translation: 提供了赋予细菌，植物，植物细胞，组织和种子的杀虫活性的组合物和方法。 提供了包含δ-内毒素多肽的编码序列的组合物。 编码序列可用于DNA构建体或表达盒中，用于在植物和细菌中转化和表达。 组合物还包括转化的细菌，植物，植物细胞，组织和种子。 特别地，提供了分离的δ-内毒素核酸分子。 另外，包括对应于多核苷酸的氨基酸序列，以及与这些氨基酸序列特异性结合的抗体。 特别地，本发明提供了分离的核酸分子，其包含编码SEQ ID NO：13-24所示的氨基酸序列的核苷酸序列或SEQ ID NO：1-12和25-44所示的核苷酸序列，作为 以及其变体和片段。

公开(公告)号：WO2010075498A3

公开(公告)日：2010-07-01

申请号：PCT/US2009/069381

申请日：2009-12-23

Applicant: ATHENIX CORPORATION ,  SAMPSON, Kimberly, S. ,  TOMSO, Daniel, J. ,  HEINRICHS, Volker

Inventor： SAMPSON, Kimberly, S. ,  TOMSO, Daniel, J. ,  HEINRICHS, Volker

IPC: C12N15/82 ,  C07K14/325

Abstract: Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions including a coding sequence for pesticidal polypeptides are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also include transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated pesticidal nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated nucleic acid molecules having nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:2, the nucleotide sequence set forth in SEQ ID NO: 1, 3, 4, 5, 6, 7, 8, 9, 10, 11, or 12, as well as variants and fragments thereof.

公开(公告)号：WO2008002962A3

公开(公告)日：2008-01-03

申请号：PCT/US2007/072203

申请日：2007-06-27

Applicant: ATHENIX CORPORATION ,  PETER, Cheryl ,  VANDE BERG, Brian ,  CARR, Brian ,  TOMSO, Daniel, John

Inventor： PETER, Cheryl ,  VANDE BERG, Brian ,  CARR, Brian ,  TOMSO, Daniel, John

IPC: C12N9/10 ,  C12N15/54 ,  C12N15/82 ,  A01H5/00

Abstract: Compositions and methods for conferring herbicide resistance to bacteria, plants, plant cells, tissues and seeds are provided. Compositions include nucleic acid molecules encoding herbicide resistance or tolerance polypeptides, vectors comprising those nucleic acid molecules, and host cells comprising the vectors. The nucleotide sequences of the invention can be used in DNA constructs or expression cassettes for transformation and expression in organisms, including microorganisms and plants. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, the present invention provides for isolated nucleic acid molecules comprising the nucleotide sequence set forth in SEQ ID NO: 1 or 14, a nucleotide sequence encoding the amino acid sequence shown in SEQ ID NO:2, the herbicide resistance nucleotide sequence deposited in a bacterial host as Accession Nos. NRRL B-30931, as well as variants and fragments thereof.

公开(公告)号：WO2007146767A2

公开(公告)日：2007-12-21

申请号：PCT/US2007/070690

申请日：2007-06-08

Applicant: ATHENIX CORPORATION ,  DUCK, Nicholas, B. ,  HINSON, Todd, K. ,  BEILINSON, Vadim ,  SCHOUTEN, Laura, Cooper ,  TOMSO, Daniel, John

Inventor： DUCK, Nicholas, B. ,  HINSON, Todd, K. ,  BEILINSON, Vadim ,  SCHOUTEN, Laura, Cooper ,  TOMSO, Daniel, John

IPC: C12N9/00 ,  C12N15/52 ,  C12N15/82 ,  A01H5/00

CPC classification number: C12N15/8277 ,  C12N9/93

Abstract: Compositions and methods for conferring herbicide resistance to and improving nitrogen utilization of bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a polypeptide that confers resistance or tolerance to herbicidal glutamine synthetase inhibitors are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated polynucleotides corresponding to herbicidal glutamine synthetase inhibitor-resistant polynucleotides are provided. Additionally, polypeptides corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated polynucleotides comprising a variant of SEQ ID NO: 1, wherein the variant polynucleotide encodes a polypeptide that is resistant to inhibition by herbicidal glutamine synthetase inhibitor.

Abstract translation: 提供了赋予除草剂对细菌，植物，植物细胞，组织和种子的氮利用的抗性和改善的组合物和方法。 提供了包含赋予除草谷氨酰胺合成酶抑制剂的抗性或耐受性的多肽的编码序列的组合物。 编码序列可用于DNA构建体或表达盒中用于在植物中转化和表达。 组合物还包括转化的细菌，植物，植物细胞，组织和种子。 特别地，提供了对应于除草谷氨酰胺合成酶抑制剂抗性多核苷酸的分离的多核苷酸。 另外，包括对应于多核苷酸的多肽。 特别地，本发明提供了包含SEQ ID NO：1的变体的分离的多核苷酸，其中所述变体多核苷酸编码对除草谷氨酰胺合成酶抑制剂具有抑制作用的多肽。

公开(公告)号：WO2006101938A1

公开(公告)日：2006-09-28

申请号：PCT/US2006/009428

申请日：2006-03-16

Applicant: ATHENIX CORPORATION ,  HAMMER, Philip, E.

Inventor： HAMMER, Philip, E.

IPC: C12N15/82 ,  A01H5/00

CPC classification number: C12N15/8216

Abstract: The present invention provides compositions and methods for regulating expression of heterologous nucleotide sequences in a plant. Compositions include a novel promoter nucleotide sequence for the gene encoding ubiquitin in Tripsacum dactyloides, as well as vectors, microorganisms, plants and plant cells comprising the promoter nucleotide sequence, or variants and fragments thereof. Methods for expressing a heterologous nucleotide sequence in a plant using the promoter sequences disclosed herein are also provided. The methods comprise stably incorporating into the genome of a plant cell a nucleotide sequence operably linked to the promoter of the present invention and regenerating a stably transformed plant that expresses the nucleotide sequence.

Abstract translation: 本发明提供调节植物中异源核苷酸序列表达的组合物和方法。 组合物包括用于编码Tripsacum dactyloides中的泛素的基因的新型启动子核苷酸序列，以及包含启动子核苷酸序列或其变体和片段的载体，微生物，植物和植物细胞。 还提供了使用本文公开的启动子序列在植物中表达异源核苷酸序列的方法。 所述方法包括将与本发明的启动子可操作地连接的核苷酸序列稳定地并入植物细胞的基因组中，并再生表达核苷酸序列的稳定转化的植物。

公开(公告)号：WO2006083891A2

公开(公告)日：2006-08-10

申请号：PCT/US2006/003434

申请日：2006-01-31

Applicant: ATHENIX CORPORATION ,  CAROZZI, Nadine ,  HARGISS, Tracy ,  KOZIEL, Michael, G. ,  DUCK, Nicholas, B.

Inventor： CAROZZI, Nadine ,  HARGISS, Tracy ,  KOZIEL, Michael, G. ,  DUCK, Nicholas, B.

IPC: A01N63/02

CPC classification number: C07K14/325 ,  A01N63/02 ,  C12N15/8281 ,  C12N15/8285 ,  C12N15/8286 ,  Y02A40/162 ,  Y02A40/164

Abstract: Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a delta-endotoxin polypeptide are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated delta-endotoxin nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID N0: 2, 4, or 6, or the nucleotide sequence set forth in SEQ ID NO: 1, 3, or 5, as well as variants and fragments thereof.

Abstract translation: 提供了赋予细菌，植物，植物细胞，组织和种子杀虫活性的组合物和方法。 提供了包含δ-内毒素多肽的编码序列的组合物。 编码序列可用于DNA构建体或表达盒中用于在植物和细菌中转化和表达。 组合物还包含转化的细菌，植物，植物细胞，组织和种子。 具体而言，提供了分离的δ-内毒素核酸分子。 另外，包含对应于多核苷酸的氨基酸序列。 特别地，本发明提供了分离的核酸分子，其包含编码SEQ ID NO：2,4或6所示的氨基酸序列或SEQ ID NO：1,3或5所示的核苷酸序列的核苷酸序列 ，以及它们的变体和片段。

公开(公告)号：WO2004097013A1

公开(公告)日：2004-11-11

申请号：PCT/US2004/007180

申请日：2004-03-10

Applicant: ATHENIX CORPORATION ,  HAMMER, Philip, E. ,  HINSON, Todd, K. ,  CARR, Brian ,  DUCK, Nicholas, B.

Inventor： HAMMER, Philip, E. ,  HINSON, Todd, K. ,  CARR, Brian ,  DUCK, Nicholas, B.

IPC: C12N9/88

CPC classification number: C12N15/8275 ,  C12N9/88

Abstract: Compositions and methods for conferring herbicide resistance to plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a polypeptide that confers resistance or tolerance to glyphosate herbicides are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants. Compositions also comprise transformed plants, plant cells, tissues, and seeds. In particular, isolated nucleic acid molecules corresponding to glyphosate resistant nucleic acid sequences are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding an amino acid sequence shown in SEQ ID NO:3, 6, 8, 11, 19, or 21, or a nucleotide sequence set forth in SEQ ID NO:1, 2, 4, 5, 7, 9, 10, 18, or 20, as well as variants and fragments thereof.

Abstract translation: 提供了赋予对植物，植物细胞，组织和种子的除草剂抗性的组合物和方法。 提供了包含赋予对草甘膦除草剂的抗性或耐受性的多肽的编码序列的组合物。 编码序列可用于DNA构建体或表达盒中用于在植物中转化和表达。 组合物还包括转化的植物，植物细胞，组织和种子。 特别地，提供了对应于草甘膦抗性核酸序列的分离的核酸分子。 另外，包括对应于多核苷酸的氨基酸序列。 特别地，本发明提供了分离的核酸分子，其包含编码SEQ ID NO：3,6,8,11,19或21所示氨基酸序列的核苷酸序列或SEQ ID NO： 1，2，4，5，7，9，10，18或20，以及其变体和片段。

公开(公告)号：WO1992001256A1

公开(公告)日：1992-01-23

申请号：PCT/US1991004691

申请日：1991-07-02

Applicant: ATHENIX CORPORATION

Inventor： ATHENIX CORPORATION ,  GARMAN, Jonathan, D.

IPC: G06F12/10

CPC classification number: G09G5/14 ,  G06F3/1423 ,  G09G5/363 ,  G09G5/393 ,  G09G2360/121

Abstract: In accordance with the present invention, an address mapper (160) is provided between the CPU (100) running an MS-DOS application program and a frame buffer (159), so as to allow the frame buffer to be mapped into the CPU address space expected by an MS-DOS application program. A write operation by the MS-DOS application program into the CPU's (100) display address space is translated by the address mapper to be performed in the address space of the frame buffer, providing to the user the appearance as if the frame buffer is part of the CPU's address space ('Virtual MS-DOS display'). This frame buffer (159) may have an address space larger than that used by the MS-DOS application program for writing to the display. This additional space can be allocated to other processes, which are each protected from inadvertently reading or writing into another process' allocated memory space.

公开(公告)号：WO2010091230A1

公开(公告)日：2010-08-12

申请号：PCT/US2010/023291

申请日：2010-02-05

Applicant: ATHENIX CORPORATION ,  HEINRICHS, Volker

Inventor： HEINRICHS, Volker

IPC: C12N15/82 ,  C07K14/325 ,  A01H5/00

CPC classification number: C12N15/8286 ,  C07K14/325 ,  Y02A40/162

Abstract: Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for pesticidal polypeptides are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, nucleic acid molecules encoding variant Cry3 sequences are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed.

Abstract translation: 提供了赋予细菌，植物，植物细胞，组织和种子的杀虫活性的组合物和方法。 提供了包含杀虫多肽的编码序列的组合物。 编码序列可用于DNA构建体或表达盒中，用于在植物和细菌中转化和表达。 组合物还包括转化的细菌，植物，植物细胞，组织和种子。 特别地，提供编码变体Cry3序列的核酸分子。 另外，包括对应于多核苷酸的氨基酸序列。

公开(公告)号：WO2010075352A1

公开(公告)日：2010-07-01

申请号：PCT/US2009/069144

申请日：2009-12-22

Applicant: ATHENIX CORPORATION ,  SAMPSON, Kimberly, S. ,  TOMSO, Daniel, J. ,  GUO, Rong

Inventor： SAMPSON, Kimberly, S. ,  TOMSO, Daniel, J. ,  GUO, Rong

IPC: C12N15/82 ,  C07K14/195 ,  A01H5/00

CPC classification number: C12N15/8286 ,  C07K14/195 ,  C07K14/325 ,  C07K14/345 ,  Y02A40/162

Abstract: Compositions and methods for conferring insecticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions including a coding sequence for a Brevibacillus-derived delta-endotoxin polypeptide are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also include transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated delta-endotoxin nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed, and antibodies specifically binding to those amino acid sequences. In particular, the present invention provides for isolated nucleic acid molecules having nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:2, 4, 7, or 10, or the nucleotide sequence set forth in SEQ ID NO: 1, 3, 5, 6, 8, 9, 11, 12, 13, 14, or 15, as well as variants and fragments thereof.

Abstract translation: 提供了赋予细菌，植物，植物细胞，组织和种子的杀虫活性的组合物和方法。 提供了包含短杆菌属来源的δ-内毒素多肽的编码序列的组合物。 编码序列可用于DNA构建体或表达盒中，用于在植物和细菌中转化和表达。 组合物还包括转化的细菌，植物，植物细胞，组织和种子。 特别地，提供了分离的δ-内毒素核酸分子。 另外，包括对应于多核苷酸的氨基酸序列，以及与这些氨基酸序列特异性结合的抗体。 特别地，本发明提供了具有编码SEQ ID NO：2,4,7或10所示的氨基酸序列的核苷酸序列的分离的核酸分子或者SEQ ID NO： 5，6，8，9，11，12，13，14或15，以及其变体和片段。