公开(公告)号：WO2019051503A2

公开(公告)日：2019-03-14

申请号：PCT/US2018/050514

申请日：2018-09-11

Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA ,  LOQUE, Dominique ,  SHIH, Patrick, M.

Inventor： LOQUE, Dominique ,  SHIH, Patrick, M.

IPC: A01H6/00 ,  C12N15/82

CPC classification number: C12N15/8216

Abstract: The present invention provides for a genetically modified plant cell or plant, comprising: (a) (i) one or more nucleic acids each encoding one or more transcription factors (or transcription activators) operatively linked to a first tissue-specific or inducible promoter, (ii) one or more nucleic acids each encoding one or more transcription repressors each operatively linked to a second tissue-specific or inducible promoter, or (iii) combinations thereof; and (b) one or more nucleic acids each encoding one or more independent genes of interest (GOI) each operatively linked to a promoter that is activated by the one or more transcription factors (or transcription activators), repressed by the one or more transcription repressors, or a combination of both.

公开(公告)号：WO2017143080A2

公开(公告)日：2017-08-24

申请号：PCT/US2017/018196

申请日：2017-02-16

Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

Inventor： MAYFIELD, Stephen P. ,  SCRANTON, Melissa ,  GEORGIANNA, David Ryan ,  OSTRAND, Joseph ,  BERNDT, Anthony Jason Erich

IPC: C12Q1/68

CPC classification number: C12N15/8216 ,  C07K14/405 ,  C12N15/63 ,  C12N15/79

公开(公告)号：WO2017093317A1

公开(公告)日：2017-06-08

申请号：PCT/EP2016/079276

申请日：2016-11-30

Applicant: UNIVERSITÄT BASEL

Inventor： BUCHER, Etienne ,  THIEME, Michael

IPC: C12N15/01 ,  A61K31/7068 ,  C07H19/06 ,  A61L31/16 ,  A01H1/06 ,  A01H3/04

CPC classification number: A01H3/04 ,  A01H1/06 ,  C12N15/01 ,  C12N15/8201 ,  C12N15/8216

Abstract: A method for the mobilization of a transposable element is provided. The method comprises the steps of a) providing an inhibitor of DNA methylation, and/or an inhibitor of transcription, and b) contacting the inhibitor(s) with a cell comprising inactivated transposable elements, yielding a cell with mobilized transposable elements. In a second aspect of the invention a method for increasing the genetic and/or epigenetic variation in a plurality of eukaryotic organisms is provided. The method comprises the steps of i. providing an inhibitor of DNA methylation and/or an inhibitor of transcription, ii. contacting the organism with the inhibitor(s) and iii. propagating the organism.

Abstract translation: 提供了用于调动转座因子的方法。 所述方法包括以下步骤：a）提供DNA甲基化抑制剂和/或转录抑制剂，和b）使所述抑制剂与包含失活转座因子的细胞接触，产生具有动员的转座因子的细胞。 在本发明的第二方面中，提供了用于增加多种真核生物中的遗传和/或表观遗传变异的方法。 该方法包括以下步骤：i。 提供DNA甲基化抑制剂和/或转录抑制剂，ii。 使生物体与抑制剂接触并且iii。 宣传有机体。

公开(公告)号：WO2017019998A1

公开(公告)日：2017-02-02

申请号：PCT/US2016/044830

申请日：2016-07-29

Applicant: UWM RESEARCH FOUNDATION, INC.

Inventor： ZHAO, Dazhong ,  HUANG, Jian

IPC: C12N15/82 ,  C12N15/113 ,  C12N9/16 ,  C07K14/415 ,  A01H5/00

CPC classification number: C12N15/8287 ,  C12N15/8216 ,  C12N15/8218

Abstract: Disclosed herein are compositions and methods for creating sterile plants by genetically ablating microspore and megaspore mother cells. Also disclosed herein are methods of restoring fertility of sterile male and female plants.

Abstract translation: 本文公开了通过遗传性消融小孢子和大孢子母细胞产生无菌植物的组合物和方法。 本文还公开了恢复无菌雄性和雌性植物的生育力的方法。

公开(公告)号：WO2016210393A1

公开(公告)日：2016-12-29

申请号：PCT/US2016/039486

申请日：2016-06-27

Applicant: PIONEER HI-BRED INTERNATIONAL, INC.

Inventor： ABBITT, Shane E.

IPC: C12N15/11 ,  C12N15/82 ,  A01H1/02

CPC classification number: C12N15/8216 ,  C12N2310/20

Abstract: The disclosure relates to gene expression regulatory sequences from Sorghum and Setaria, specifically to the regulatory regions of GOS2 gene and fragments thereof and their use in modulating the expression of one or more heterologous nucleic acid fragments in plants. The disclosure further discloses compositions, polynucleotide constructs, transformed host cells, plants and seeds containing the recombinant construct with the promoter, and methods for preparing and using the same.

Abstract translation: 本公开涉及来自高粱和狗尾草的基因表达调控序列，特别是GOS2基因及其片段的调节区及其在调节植物中一个或多个异源核酸片段表达的用途。 本公开进一步公开了组合物，多核苷酸构建体，转化的宿主细胞，含有重组构建体的启动子的植物和种子，及其制备和使用方法。

公开(公告)号：WO2016168230A2

公开(公告)日：2016-10-20

申请号：PCT/US2016027188

申请日：2016-04-13

Applicant: DOW AGROSCIENCES LLC

Inventor： KUMAR SANDEEP ,  HEMINGWAY DAREN ,  AUSMUS CARLA ,  WORDEN ANDREW F ,  ASBERRY ANDREW

IPC: A01H5/00

CPC classification number: C12N15/8227 ,  C07K14/825 ,  C12N15/8216

Abstract: This disclosure concerns compositions and methods for promoting transcription of a nucleotide sequence in a plant or plant cell, employing a promoter from a Panicum virgatum metallothionein-like gene (mtl). Some embodiments relate to a promoter from a Panicum virgatum metallothionein-like gene (mtl) that functions in plants to promote transcription of operably linked nucleotide sequences.

Abstract translation: 本公开涉及使用来自黍Panicum virgatum金属硫蛋白样基因（mtl）的启动子促进植物或植物细胞中核苷酸序列转录的组合物和方法。 一些实施方案涉及在植物中起作用以促进可操作地连接的核苷酸序列的转录的来自黍Panicum virgatum金属硫蛋白样基因（mtl）的启动子。

公开(公告)号：WO2016127075A3

公开(公告)日：2016-09-15

申请号：PCT/US2016016811

申请日：2016-02-05

Applicant: UNIV NEW YORK

Inventor： CORUZZI GLORIA ,  BIRNBAUM KENNETH ,  BARGMANN BASTIAAN ,  KROUK GABRIEL ,  KATARI MANPREET ,  OBERTELLO MARIANA

IPC: A01H5/00 ,  C12N15/82

CPC classification number: C12N15/8271 ,  C12N15/8216 ,  C12N15/8217 ,  C12N15/8261 ,  C12Q1/6895 ,  C12Q2600/13 ,  C12Q2600/156 ,  Y02A40/146

Abstract: Plant genes regulated by transcription factors that control the gene network response to an environmental perturbation or signal are described. This class of genes responds to the perturbation of a transcription factor and the signal it transduces, but surprisingly, without stable binding of the transcription factor. These genes represent members of the "dark matter" of metabolic regulatory circuits. The invention involves the transgenic manipulation of these "response genes" and/or the genes encoding their regulatory transcription factors in plants so that their respective gene products are either overexpressed or underexpressed in the plant in order to confer a desired phenotype. The invention also relates to a rapid technique named "TARGET" ( T ransient A ssay R eporting G enome-wide E ffects of T ranscription factors) for determining such "response genes" and their transcription factors by perturbation of the expression of the transcription factors of interest in protoplasts of any plant species.

Abstract translation: 描述了通过控制基因网络对环境扰动或信号的响应的转录因子调控的植物基因。 这类基因对转录因子的扰动及其转导的信号作出反应，但令人惊讶的是，转录因子没有稳定的结合。 这些基因代表了代谢调节电路的“暗物质”成员。 本发明涉及对植物中这些“应答基因”和/或编码其调控转录因子的基因的转基因操纵，使得它们各自的基因产物在植物中过表达或未表达，以产生所需的表型。 本发明还涉及一种称为“TARGET”（即“T”）的快速技术，其中，“U” 用于通过扰动感兴趣的转录因子在任何植物物种的原生质体中的表达来确定这样的“应答基因”及其转录因子来确定这些“转录因子的广泛不良效应” 。

公开(公告)号：WO2016139682A1

公开(公告)日：2016-09-09

申请号：PCT/IN2016/050078

申请日：2016-03-04

Applicant: MAHARASHTRA HYBRID SEEDS COMPANY LIMITED (MAHYCO)

Inventor： KHANALE, Vaishali Praveen ,  BHATTACHARYA, Anjanabha ,  BANDYOPADHYAY, Anindya

IPC: C12N15/82 ,  C12N15/09

CPC classification number: C12N15/8238 ,  C12N15/8216 ,  C12N15/8237

Abstract: The present disclosure provides a cotton promoter, designated "p2", which exhibits promoter activity. Interestingly, the promoter is also influenced by water or salt stress. Deletion analysis reveals upstream elements/motifs in the promoter which influence promoter activity, and sequences that are potentially responsive to salt or water stress.

Abstract translation: 本公开提供了表达启动子活性的称为“p2”的棉花启动子。 有趣的是，启动子也受水或盐胁迫的影响。 缺失分析揭示了影响启动子活性的启动子中的上游元件/基序，以及潜在地对盐或水胁迫有反应的序列。

公开(公告)号：WO2016134081A1

公开(公告)日：2016-08-25

申请号：PCT/US2016/018358

申请日：2016-02-17

Applicant: IOWA STATE UNIVERSITY RESEARCH FOUNDATION, INC.

Inventor： LI, Ling ,  WURTELE, Eve Syrkin

IPC: A01H5/00 ,  C07K14/415 ,  C12N15/82

CPC classification number: C12N15/8251 ,  A01H1/02 ,  A01H5/00 ,  C07K14/415 ,  C12N15/8205 ,  C12N15/8216 ,  C12N15/8241 ,  C12N15/8247 ,  C12N15/8279

Abstract: Method of increasing protein content in a eukaryotic cell comprising an NF-YC4 gene comprising modifying the transcriptional repressor binding site; method of producing a plant with increased protein content comprising crossing and selecting for increased protein content; method of increasing resistance to a pathogen or a pest in a plant comprising an NF-YC4 gene comprising modifying the transcriptional repressor binding site, alone or in further combination with expressing QQS in the plant; method for producing a plant with increased resistance to a pathogen or a pest comprising crossing and selecting for increased resistance to the pathogen or the pest; a cell, collection of cells, tissue, organ, or organism in which the NF-YC4 gene comprises a promoter comprising a transcriptional repressor binding site that has been modified so that the transcriptional repressor cannot prevent transcription of the NF-YC4; hybrid plants; and seeds.

Abstract translation: 包含NF-YC4基因的真核细胞中增加蛋白质含量的方法，包括修饰转录阻遏物结合位点; 产生蛋白质含量增加的植物的方法，包括交叉和选择增加的蛋白质含量; 在包含NF-YC4基因的植物中增加对病原体或害虫的抗性的方法，包括单独或与植物中表达QQS的进一步组合修饰转录阻遏物结合位点; 用于生产对病原体或害虫具有增加的抗性的植物的方法，包括交叉和选择增加的对病原体或害虫的抗性; 细胞，细胞，组织，器官或生物体的集合，其中NF-YC4基因包含启动子，其包含经修饰的转录阻遏物结合位点，使得转录阻遏物不能阻止NF-YC4的转录; 杂交植物; 和种子。

公开(公告)号：WO2016127075A2

公开(公告)日：2016-08-11

申请号：PCT/US2016/016811

申请日：2016-02-05

Applicant: NEW YORK UNIVERSITY

Inventor： CORUZZI, Gloria ,  BIRNBAUM, Kenneth ,  BARGMANN, Bastiaan ,  KROUK, Gabriel ,  KATARI, Manpreet ,  OBERTELLO, Mariana

IPC: A01H5/00 ,  C12N15/82

CPC classification number: C12N15/8271 ,  C12N15/8216 ,  C12N15/8217 ,  C12N15/8261 ,  C12Q1/6895 ,  C12Q2600/13 ,  C12Q2600/156 ,  Y02A40/146

Abstract: Plant genes regulated by transcription factors that control the gene network response to an environmental perturbation or signal are described. This class of genes responds to the perturbation of a transcription factor and the signal it transduces, but surprisingly, without stable binding of the transcription factor. These genes represent members of the "dark matter" of metabolic regulatory circuits. The invention involves the transgenic manipulation of these "response genes" and/or the genes encoding their regulatory transcription factors in plants so that their respective gene products are either overexpressed or underexpressed in the plant in order to confer a desired phenotype. The invention also relates to a rapid technique named "TARGET" ( T ransient A ssay R eporting G enome-wide E ffects of T ranscription factors) for determining such "response genes" and their transcription factors by perturbation of the expression of the transcription factors of interest in protoplasts of any plant species.

Abstract translation: 描述了由控制基因网络对环境扰动或信号响应的转录因子调控的植物基因。 这类基因对转录因子及其转导信号的干扰作出反应，但令人惊讶的是，没有转录因子的稳定结合。 这些基因代表“暗物质”的成员。 的代谢调节电路。 本发明涉及这些“应答基因”的转基因操作。 和/或在植物中编码其调节转录因子的基因，以使其各自的基因产物在植物中过表达或低表达以赋予期望的表型。 本发明还涉及名为“TARGET”的快速技术。 （> >> >> > > enome-wide 用于确定转录因子的E / E转录因子） 这样的“响应基因” 和他们的转录因子，通过扰乱在任何植物物种的原生质体中感兴趣的转录因子的表达。