公开(公告)号：WO2011000833A1

公开(公告)日：2011-01-06

申请号：PCT/EP2010/059212

申请日：2010-06-29

Applicant: UNIVERSITÄTSKLINIKUM HEIDELBERG ,  MEDIZINISCHE UNIVERSITÄT GRAZ ,  WAGNER, Wolfgang ,  BORK, Simone ,  HORN, Patrick ,  SCHALLMOSER, Katharina ,  ROHDE, Eva ,  BARTMANN, Christina ,  HO, Anthony, Dick ,  STRUNK, Dirk

Inventor： WAGNER, Wolfgang ,  BORK, Simone ,  HORN, Patrick ,  SCHALLMOSER, Katharina ,  ROHDE, Eva ,  BARTMANN, Christina ,  HO, Anthony, Dick ,  STRUNK, Dirk

IPC: G01N33/50

CPC classification number: G01N33/5091 ,  C12Q1/6881 ,  C12Q2600/158 ,  G01N2333/4703

Abstract: The present invention relates to the field of diagnostics. Specifically, it relates to means and methods for determining the replicative senescence status of a cell culture and for assessing the suitability of a cell culture for therapeutic uses.

Abstract translation: 本发明涉及诊断领域。 具体地说，本发明涉及用于确定细胞培养物的复制衰老状态的方法和方法，并且用于评估细胞培养物用于治疗用途的适用性。

公开(公告)号：WO2008110570A1

公开(公告)日：2008-09-18

申请号：PCT/EP2008/052933

申请日：2008-03-12

Applicant: MEDIZINISCHE UNIVERSITÄT GRAZ ,  STRUNK, Dirk ,  ROHDE, Eva ,  BARTMANN, Christina ,  SCHALLMANN, Katharina

Inventor： STRUNK, Dirk ,  ROHDE, Eva ,  BARTMANN, Christina ,  SCHALLMANN, Katharina

IPC: G01N33/50 ,  C12N5/06

CPC classification number: C12N5/0692 ,  C12N2501/165 ,  G01N33/5005 ,  G01N33/5064

Abstract: The present invention provides a simple and rapid method for determining colony forming units (CFU) of endothelial progenitor cells (EPC). Furthermore, a method for identifying compounds exerting an effect on those CFU of EPC is provided. The present invention also provides medium for culturing endothelial cells which is comprised of EGM-2, hydrocortisone and VEGF as well as human platelet lysate (HPL) as supplement.

Abstract translation: 本发明提供了一种用于测定内皮祖细胞（EPC）的集落形成单位（CFU）的简单且快速的方法。 此外，提供了鉴定对EPC的CFU产生影响的化合物的方法。 本发明还提供用于培养由EGM-2，氢化可的松和VEGF以及人血小板裂解物（HPL）作为补充物的内皮细胞的培养基。