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    파브리 병의 유도-만능 줄기세포 모델 및 이의 용도
    4.
    发明申请
    파브리 병의 유도-만능 줄기세포 모델 및 이의 용도 审中-公开
    诱导的PLUIPOTETI STEM细胞模型用于制造疾病和其使用

    公开(公告)号：WO2015099206A1

    公开(公告)日：2015-07-02

    申请号：PCT/KR2013/012020

    申请日：2013-12-23

    Applicant: 한국과학기술원

    Inventor： 한용만 ,  박상욱

    IPC: C12N5/074 ,  C12Q1/68 ,  G01N33/15

    CPC classification number: C12N5/0696 ,  C12N2501/602 ,  C12N2501/603 ,  C12N2501/604 ,  C12N2501/606 ,  C12N2506/1307 ,  G01N33/5064

    Abstract: 본 발명은 파브리 병(Fabry disease)의 유도-만능 줄기세포(induced pluripotent stem cells; iPSC) 모델, 이의 제조 방법, 및 상기 iPSC 모델을 파브리 병의 발병 연구 및 치료제 스크리닝 방법에 이용하는 용도에 관한 것으로, 구체적으로 파브리 병 환자의 섬유아세포로부터 파브리 병 유래의 유도-만능 줄기세포(induced pluripotent stem cells; iPSC), 배상체(embryoid body, EB) 및 혈관 세포(vascular cell)의 발생 및 분화를 유도하였으며, 상기 파브리 병 유래의 iPSC는 GLA 단백질의 발현 및 활성이 정상 세포에 비하여 현저히 감소하여 글로보트리아오실세라마이드(globotriaosylceramide, Gb3, CD77)가 축적되는 것을 확인하였고, 파브리 병 유래의 iPSC로부터 혈관 세포로 분화를 유도한 결과, 혈관내피 세포 및 혈관 평활근 세포로 분화되어 마커 단백질을 유의적으로 발현하였으며, 상기 혈관내피 세포 및 혈관 평활근 세포에 파브라자임 효소를 처리하였을 때, Gb3의 축적이 유의적으로 감소하므로, 상기 세포 모델은 파브리 병의 기전 분석 연구 및 치료제 스크리닝 방법을 위한 분석 연구에 유용하게 사용될 수 있다.

    Abstract translation: 本发明涉及用于法布里病的诱导多能干细胞（iPSC）模型及其制备方法，以及在研究法布里病发病机理中使用iPSC模型的用途，以及用于筛选法布里病治疗 剂。 具体来说，通过从法布里病患者的成纤维细胞诱导Fabry疾病衍生的iPSC，胚状体（EB）和血管细胞的产生和分化，并确认了GLA的表达和活性，从而确认了globotriaosylceramide（Gb3，CD77）的积累 与正常细胞相比，法布里病衍生的iPSCs中的蛋白质显着降低，并且当通过将Fabry疾病衍生的iPSC分化为血管内皮细胞和血管平滑肌细胞作为诱导分化的结果显着表达标记蛋白时 将法布里病衍生的iPSCs进入血管细胞，用fabrazyme处理血管内皮细胞和血管平滑肌细胞，从而显着减少Gb3的积累，iPSC模型可有效地用于法布里病的致病机制的分析研究 以及法布里病筛选方法的分析研究 治疗剂。

    CELL-BASED CONTROL AND METHOD
    5.
    发明申请
    CELL-BASED CONTROL AND METHOD 审中-公开
    基于细胞的控制和方法

    公开(公告)号：WO2015017535A2

    公开(公告)日：2015-02-05

    申请号：PCT/US2014/048884

    申请日：2014-07-30

    Applicant: SIEMENS HEALTHCARE DIAGNOSTICS INC.

    Inventor： PUGIA, Michael ,  MARFURT, Karen, L.

    IPC: G01N33/574

    CPC classification number: G01N33/5023 ,  C12N5/0602 ,  G01N33/5005 ,  G01N33/5044 ,  G01N33/5064 ,  G01N33/574 ,  G01N33/96 ,  G01N2001/2893

    Abstract: Control cells include a stable intact cell and, on the surface of the stable intact cell, a plurality of different antigens. Each of the different antigens corresponds to an antigen of interest. The control cells are employed in methods of evaluating the efficacy of a biological procedure performed on a sample. The method comprises conducting the biological procedure in the presence of the control cells. The control cells are examined to determine the efficacy of the biological procedure.

    Abstract translation: 对照细胞包括稳定的完整细胞，并且在稳定的完整细胞的表面上具有多种不同的抗原。 每种不同的抗原对应于感兴趣的抗原。 对照细胞用于评估对样品进行的生物学过程的功效的方法。 该方法包括在存在对照细胞的情况下进行生物学过程。 检查对照细胞以确定生物学程序的功效。

    PORTED PARALLEL PLATE FLOW CHAMBER AND METHODS FOR USE THEREOF
    6.
    发明申请
    PORTED PARALLEL PLATE FLOW CHAMBER AND METHODS FOR USE THEREOF 审中-公开
    多孔平行板流动室及其使用方法

    公开(公告)号：WO2014143696A1

    公开(公告)日：2014-09-18

    申请号：PCT/US2014/027764

    申请日：2014-03-14

    Applicant: RINKER, Kristina, D. ,  SHEPHERD, Robert, D.

    Inventor： RINKER, Kristina, D. ,  SHEPHERD, Robert, D.

    IPC: G01N33/483 ,  G01N33/487 ,  C12M1/00

    CPC classification number: G01N33/5023 ,  B01L3/502 ,  B01L3/5025 ,  B01L2200/025 ,  B01L2200/026 ,  B01L2200/027 ,  B01L2200/0689 ,  B01L2300/044 ,  B01L2300/0609 ,  B01L2300/0627 ,  B01L2300/0822 ,  B01L2300/0829 ,  B01L2300/0877 ,  B01L2400/0487 ,  B01L2400/086 ,  C12M35/04 ,  C12M41/46 ,  C12Q1/6876 ,  C12Q2600/158 ,  G01N33/487 ,  G01N33/5064

    Abstract: Flow chambers are provided. In some embodiments, the flow chambers include an inner panel having at least one flow channel having an inlet/outlet opening on each end thereof formed therein, wherein the inlet/outlet openings are adapted to releasably receive a septum; one or more ports adapted to releasably receive a plug and for at least liquid communication with the at least one flow channel, and an outer frame that defines an outer portion of the at least one flow channel and that defines a perimeter of the flow chamber. In some embodiments, the flow chamber has overall dimensions of a standard multiwell plate and the at least one flow channel is located in a position that corresponds to a column location of the standard multiwell plate. Also provided are methods for producing the presently disclosed flow chambers and employing the same to assay biological features of cultured cells and/or tissues.

    Abstract translation: 提供流动室。 在一些实施例中，流动室包括具有至少一个流动通道的内部板，所述至少一个流动通道在其每个端部上形成有入口/出口开口，其中所述入口/出口开口适于可释放地接收隔膜; 一个或多个端口，其适于可释放地接收塞子并且用于至少与所述至少一个流动通道的液体连通;以及外框架，其限定所述至少一个流动通道的外部部分并限定所述流动室的周边。 在一些实施例中，流动室具有标准多孔板的总体尺寸，并且至少一个流动通道位于对应于标准多孔板的柱位置的位置。 还提供了用于生产当前公开的流动室的方法，并且使用该方法来测定培养的细胞和/或组织的生物学特征。

    SKIN MODEL
    7.
    发明申请
    SKIN MODEL 审中-公开
    皮肤模型

    公开(公告)号：WO2014132063A1

    公开(公告)日：2014-09-04

    申请号：PCT/GB2014/050583

    申请日：2014-02-27

    Applicant: ALCYOMICS LTD ,  UNIVERSITY OF NEWCASTLE UPON TYNE

    Inventor： LOVAT, Penny ,  HILL, David ,  DICKINSON, Anne ,  AHMED, Shaheda

    IPC: C12N5/071 ,  A61B5/00 ,  G01N33/50

    CPC classification number: G01N33/94 ,  A61B5/411 ,  A61B5/445 ,  C12N5/0698 ,  C12N2502/094 ,  C12N2502/1323 ,  C12N2533/54 ,  G01N33/5044 ,  G01N33/5047 ,  G01N33/505 ,  G01N33/5064 ,  G01N33/5082 ,  G01N2800/24 ,  G09B23/28

    Abstract: A three dimensional (3-D) model comprising a scaffold and autologous skin cells, the invention also provides methods of predicting immunogenicity and hypersensitivity or allergic or adverse immune reactions to potential therapeutic compounds, biologies, cosmetics and chemical sensitizers using the 3-D model of skin cells. The methods provide an in vitro assay employing autologous blood derived cells in the 3-D skin equivalent model and is of particular utility in the identification and prediction of skin sensitizers and in particular agents that may cause allergic contact dermatitis. The assay of the present invention provides inter alia methods of screening library compounds for sensitizing activity, identifying optimal therapeutics, especially but not exclusively, monoclonal antibodies and kits therefor.

    Abstract translation: 包括支架和自体皮肤细胞的三维（3-D）模型，本发明还提供使用3-D模型预测免疫原性和对潜在的治疗化合物，生物制品，化妆品和化学敏化剂的过敏或过敏或不良免疫反应的方法 的皮肤细胞。 该方法提供了在3-D皮肤等效模型中使用自体血液来源的细胞的体外测定，并且在鉴定和预测皮肤敏化剂，特别是可能引起过敏性接触性皮炎的试剂中尤其有用。 本发明的测定特别提供了筛选文库化合物以增强活性，鉴定最佳治疗剂的方法，特别但非排他地，单克隆抗体及其试剂盒。

    血管透過性亢進抑制作用の評価方法
    8.
    发明申请
    血管透過性亢進抑制作用の評価方法 审中-公开
    血管超声心动图调节作用的评价方法

    公开(公告)号：WO2014129653A1

    公开(公告)日：2014-08-28

    申请号：PCT/JP2014/054528

    申请日：2014-02-25

    Applicant: 浜松ホトニクス株式会社

    Inventor： 山内　豊彦 ,  平川　聡史 ,  山下　豊 ,  戸倉　新樹

    IPC: C12Q1/02

    CPC classification number: G01N33/5026 ,  G01N33/5064

    Abstract: 　被験物質を血管内皮細胞に接触させる工程と、血管内皮細胞の血管透過性を亢進する薬剤を血管内皮細胞に接触させる工程と、血管内皮細胞の頂点高さを測定する工程とを備え、血管内皮細胞の頂点高さを被験物質の血管内皮細胞の血管透過性亢進の抑制作用の指標とする方法。

    Abstract translation: 使用测试物质中的内皮细胞的峰高作为内皮细胞中血管高渗透性调节作用的指标的方法，其包括：使测试物质与内皮细胞接触的步骤; 使内皮细胞中血管渗透性恶化的药物与内皮细胞接触的步骤; 测定内皮细胞的峰高的步骤。

    NANOPARTICLES DISPERSION FOR DETECTION OF SKIN HEALTH CONDITIONS AND A DIAGNOSTIC KIT THEREOF
    9.
    发明申请
    NANOPARTICLES DISPERSION FOR DETECTION OF SKIN HEALTH CONDITIONS AND A DIAGNOSTIC KIT THEREOF 审中-公开
    用于检测皮肤健康状况的纳米颗粒分散液及其诊断试剂盒

    公开(公告)号：WO2014108758A1

    公开(公告)日：2014-07-17

    申请号：PCT/IB2013/050322

    申请日：2013-01-14

    Applicant: ITC LIMITED

    Inventor： BISWAS, Samares Chandra ,  PANIGRAHI, Sudipa ,  MURUGESAN, Kavitha ,  SEETHARAMACHARYA, Sudheendra, U ,  RAMAMURTHI, Suresh

    IPC: G01N33/50 ,  B82Y15/00 ,  G01N33/52 ,  A61B10/00

    CPC classification number: B82Y15/00 ,  A61B2010/0006 ,  G01N33/5005 ,  G01N33/5064 ,  G01N33/52

    Abstract: Nanoparticles dispersion is provided for diagnosing skin health conditions comprising at least three components selected from glucose, chloroauric acid, potassium hydroxide, sodium borohydride, and water, wherein said nanoparticle dispersion has a particle size of 1 to 100 nm. A test kit is provided for diagnosing skin health conditions such as normal, dry or dandruff comprising nanoparticles dispersion comprising at least three components selected from glucose, chloroauric acid, potassium hydroxide, sodium borohydride, and water. A method of diagnosing the skin health conditions comprising the steps of collecting the skin test sample and treatment with nanoparticle and measuring the color of test samples and diagnosing the skin dryness.

    Abstract translation: 提供纳米颗粒分散体用于诊断包含至少三种选自葡萄糖，氯金酸，氢氧化钾，硼氢化钠和水的组分的皮肤健康状况，其中所述纳米颗粒分散体具有1至100nm的粒度。 提供用于诊断皮肤健康状况如正常，干燥或头皮屑的测试试剂盒，其包含至少三种选自葡萄糖，氯金酸，氢氧化钾，硼氢化钠和水的组分的纳米颗粒分散体。 一种诊断皮肤健康状况的方法，包括收集皮肤测试样品和用纳米颗粒处理并测量测试样品的颜色并诊断皮肤干燥的步骤。

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